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Snakegourd fruit tissue culture seedling growing method

A technology of tissue culture and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of easy variation in seed propagation, difficulty in distinguishing male and female seedlings, and uneven emergence, so as to achieve easy division and multiplication , Seedling raising efficiency improvement, the effect of good variety characteristics

Inactive Publication Date: 2018-01-12
无为县姚沟镇太白瓜蒌种植专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seed propagation is used. Since Trichosanthes is a dioecious plant, it is difficult for seedlings to distinguish between male and female. It can only be distinguished at the flowering stage, which affects the production of Trichosanthes, and seed propagation is prone to variation, and the reproductive effect is poor.
At present, root division is generally used in production, but there are problems such as low reproduction coefficient and uneven emergence.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of Trichosanthes quince tissue culture seedling raising method that the present invention proposes comprises the following steps:

[0022] S1, selection of explants: take the branch that the female Trichosanthes quinquefolium was pumped in that year, take the middle part of the branch and cut it into stem segments, each stem segment contains an axillary bud, clean the stem segment with washing powder aqueous solution, and then rinse it under running water, Disinfection and sterilization;

[0023] S2, culture of tissue-cultured seedlings: the stem section of Trichosanthes trichosanthes is placed in the culture room, and cultured in the callus-inducing medium, the proliferation medium and the rooting medium successively to obtain the tissue-cultured plantlets;

[0024] 1) Callus induction culture: inoculate the stem section of Trichosanthes mellifera into the callus induction medium for culture to obtain callus, and the callus induction medium is MS+1.0mg / L 6-BA+0....

Embodiment 2

[0030] A kind of Trichosanthes quince tissue culture seedling raising method that the present invention proposes comprises the following steps:

[0031] S1. Selection of explants: Take the branches of the female Trichosanthes in the same year, cut the middle part of the branches into 1cm stem segments, each stem segment contains an axillary bud, wash the stem segments with washing powder aqueous solution, and then rinse them under running water Clean, then soak the Trichosanthes stem section in 70% ethanol for 50 seconds, wash it twice with sterile water, then soak it in 10% sodium hypochlorite solution for 5 minutes, rinse it three times with sterile water, and blot the surface moisture with sterile paper;

[0032] S2, culture of tissue-cultured seedlings: the stem section of Trichosanthes trichosanthes is placed in the culture room, and cultured in the callus-inducing medium, the proliferation medium and the rooting medium successively to obtain the tissue-cultured plantlets;...

Embodiment 3

[0039] A kind of Trichosanthes quince tissue culture seedling raising method that the present invention proposes comprises the following steps:

[0040]S1. Selection of explants: Take the branches of the female Trichosanthes in the same year, take the middle part of the branches and cut them into 1.5cm stem segments. Rinse well, then soak the Trichosanthes stem section in 70% ethanol for 55 seconds, wash it with sterile water for 3 times, then soak it with 10% sodium hypochlorite solution for 8 minutes, rinse it with sterile water for 3 times, and blot the surface moisture with sterile paper;

[0041] S2, culture of tissue-cultured seedlings: the stem section of Trichosanthes trichosanthes is placed in the culture room, and cultured in the callus-inducing medium, the proliferation medium and the rooting medium successively to obtain the tissue-cultured plantlets;

[0042] 1) Callus induction culture: inoculate the stem section of Trichosanthes mellifera into the callus inducti...

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Abstract

The invention discloses a snakegourd fruit tissue culture seedling growing method, and relates to the technical field of snakegourd fruit seedling growing. The method comprises the steps of explant selection, tissue seedling culture, nursery selection and transplantation and growing of tissue culture seedlings. According to the method, seedling growing is conducted through tissue culture, seedlinggrowing efficiency is improved greatly, seedling growing cost is reduced, and the varietal characteristics of the snakegourd fruit can be well maintained. Compared with a traditional root-divided breeding method, the reproduction coefficient can be increased by 4-5 times, the seedling growing period can be shortened by 10%, a snakegourd fruit rapid propagation technical system can be established,and seedling yield can be increased.

Description

technical field [0001] The invention relates to the technical field of trichosanthes seedling cultivation, in particular to a method for cultivating trichosanthes seedlings through tissue culture. Background technique [0002] Melon basket, also known as Trichosanthes, is a perennial herbaceous vine of Cucurbitaceae. It grows in sunny hillsides, foothills, rock crevices, and field grass. It is mainly produced in Anhui, Shandong, Henan, Sichuan, Jiangsu, Shaanxi and other places in China. Melon basket is a kind of commonly used Chinese herbal medicine. Its fruit is used as medicine and it is called Trichosanthes chinensis, its seeds are used as medicine and it is called Trichosanthes japonicus, and its roots are used as medicine and it is called Trichosanthes chinensis. [0003] Trichosanthes are usually propagated by seeds and root divisions. Seed propagation is adopted, because Trichosanthes is a dioecious plant, it is difficult for seedlings to distinguish males and femal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈效春
Owner 无为县姚沟镇太白瓜蒌种植专业合作社
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