The present application relates to the technical field of
plant induction regeneration, and particularly relates to a method for improving artificial propagation efficiency of
Trichosanthes cucumerina var. villosa, which uses
Trichosanthes cucumerina var. villosa stem segments as explants and optimizes the proliferation medium, fixes the excellent traits of the female parent to the greatest extent through direct
organogenesis, avoids the variation possibly caused by indirect
organogenesis, and has a proliferation cycle of 40 days and a propagation coefficient of more than 14.0; the method meets the needs of large-scale production of high-quality
Trichosanthes cucumerina var. villosa seedlings, solves the
bud tip
necrosis phenomenon commonly existing in
in vitro rapid propagation of
cucurbitaceae plants, improves the quality of seedlings, and improves the
economic benefits; the present application has important significance and value for artificial rapid propagation of Trichosanthes cucumerina var. villosa, and lays a technical foundation for protecting the wild resources and developing artificial planting.