The invention relates to the technical field of
plant tissue culture, and discloses an in-vitro
induction method of a
trichosanthes kirilowii maxim autotetraploid, which successfully realizes in-vitro induction and high-speed propagation of the
trichosanthes kirilowii maxim autotetraploid by vibrating and soaking a
nodal stem segment of the
trichosanthes kirilowii maxim through
colchicine. After successful doubling is determined, optimizing a tetraploid
plant artificial rapid propagation
system; finally, the multiplication coefficient of the
trichosanthes kirilowii maxim tetraploid can reach 6.43, the rooting rate is 100%, and the
survival rate can reach 92% after regenerated seedlings are domesticated and transplanted. According to the method, the
trichosanthes kirilowii maxim autotetraploid is successfully induced, an artificial efficient regeneration
system of the
trichosanthes kirilowii maxim autotetraploid is established, an excellent material is provided for new variety breeding and genetic research of trichosanthes kirilowii maxim, meanwhile, an experimental basis is provided for
ploidy breeding of other species of
cucurbitaceae, and the method has remarkable application and popularization value.