Tissue culture method of Pueraria thomsonii

A technology of tissue culture and kudzu root, applied in the field of plant tissue culture, can solve problems such as death and kudzu browning

Active Publication Date: 2019-01-25
HUAIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, it is necessary to provide a method for kudzu tissue culture that can prevent kudzu from browning in order to solve the problem that kudzu is prone to browning and death during tissue culture

Method used

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  • Tissue culture method of Pueraria thomsonii
  • Tissue culture method of Pueraria thomsonii
  • Tissue culture method of Pueraria thomsonii

Examples

Experimental program
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Embodiment 1

[0037] The present embodiment provides a kind of kudzu root tissue culture method, it comprises the following steps:

[0038] Explant treatment: take the twigs of Pueraria japonica with axillary buds, cut them into small sections of about 1 cm, and each section has axillary buds. Soak the cut twigs of kudzu root in water with Tween added for 30 minutes, and then rinse with running water for 1 hour. After washing, sterilize with 75% alcohol for 20 seconds, and then put in 0.1% HgCl 2 Soak in the solution for sterilization for 6.5 minutes, then rinse with sterile water for 5 times, and finally dry the water with sterile filter paper for later use.

[0039] Callus induction: inoculate the treated Pueraria twigs on the first medium for cultivation, the temperature of the induction environment is 23°C to 27°C, the relative humidity of the air in the induction environment is 40% to 50%, and the light treatment is alternately carried out for 12 hours And 12h dark treatment, the lig...

Embodiment 2

[0046] The present embodiment provides a kind of kudzu root tissue culture method, it comprises the following steps:

[0047] Explant treatment: take the twigs of Pueraria japonica with axillary buds, cut them into small sections of about 1 cm, and each section has axillary buds. Soak the cut twigs of kudzu root in water with Tween added for 30 minutes, and then rinse with running water for 1 hour. After washing, sterilize with 75% alcohol for 20 seconds, and then put in 0.1% HgCl 2 Soak in the solution for 6.5 minutes, then rinse with sterile water for 5 times, and finally dry the water with sterile filter paper, and set aside.

[0048] Callus induction: Inoculate the sterilized Pueraria twigs on the first medium for cultivation, the temperature of the cultivation room is 23°C-27°C, the relative humidity of the air is 40%-50%, alternately carry out 12h light treatment and 12h The light intensity of the dark treatment and the light treatment is 1500 lux, and the culture time...

Embodiment 3

[0055] The present embodiment provides a kind of kudzu root tissue culture method, it comprises the following steps:

[0056] Explant treatment: take the twigs of Pueraria japonica with axillary buds, cut them into small sections of about 1 cm, and each section has axillary buds. Soak the cut twigs of kudzu root in water with Tween added for 30 minutes, and then rinse with running water for 1 hour. After washing, sterilize with 75% alcohol for 20 seconds, and then put in 0.1% HgCl 2 Soak in the solution for 6 minutes, rinse with sterile water for 5 times, and finally dry the water with sterile filter paper for later use.

[0057] Callus induction: Inoculate the sterilized Pueraria twigs on the first medium for cultivation, the temperature of the cultivation room is 23°C-27°C, the relative humidity of the air is 40%-50%, alternately carry out 12h light treatment and 12h The light intensity of the dark treatment and the light treatment is 1700 lux, and the culture time is 7 da...

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Abstract

The invention relates to a tissue culture method of Pueraria thomsonii, comprising the steps of treating an explant, to be specific, collecting a Pueraria thomsonii twig with axillary buds, disinfecting, and killing bacteria for use; inducing a callus, to be specific, inducing the treated Pueraria thomsonii twig to a first medium for culturing to obtain the callus; inducing cluster buds, to be specific, dicing the callus, inoculating to a second medium, and culturing to obtain cluster buds; performing rooting culturing, to be specific, dividing the cluster buds to obtain single buds, transplanting the single buds to a third medium, and culturing to obtain Pueraria thomsonii plantlets. The tissue culture method of Pueraria thomsonii has the advantages that the Pueraria thomsonii twig with axillary buds having high differentiating capacity and low polyphenol content is used as an explant, different media are used in different culture stages, browning of Pueraria thomsonii during tissue culture is prevented, and survival rate of Pueraria thomsonii in tissue culture is effectively increased.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of kudzu root. Background technique [0002] Pueraria lobata has the functions of relieving the exterior and refreshing the spleen, and is a commonly used medicinal material. Puerariathomsonii Benth, a leguminous plant, is called Puerariathomsonii Benth because of its high starch content in its root tubers. Puerariathomsonii Benth has high medicinal and edible value, and can be used as a natural health food, with a large market demand. The artificial propagation of kudzu mainly adopts cutting propagation. This kind of breeding method needs to consume a large amount of kudzu stems, the reproduction coefficient is low, and the variety improvement is also difficult. In addition, due to the severe accumulation of viruses in successive years of cuttings, the yield of kudzu is reduced and the species quality is reduced. Degradation, and quality...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 贺安娜刘良科
Owner HUAIHUA UNIV
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