Culture medium and rapid propagation method for tissue culture of Vitex agnus-castus L

Abstract

The invention discloses a culture medium and a rapid propagation method for tissue culture of Vitex agnus-castus L, wherein the culture medium comprises a bud induction culture medium, a proliferationculture medium and a rooting culture medium. According to the present invention, by using the specific culture medium formula of the three components, the proliferation coefficient and the rooting rate of the tissue culture of Vitex agnus-castus L are significantly improved so as to improve the proliferation efficiency of Vitex agnus-castus L; the current growth branch of Vitex agnus-castus L isselected as the explant material, has characteristics of strong growth and strong branching ability, and does not easily produce browning during the tissue culture; and the reproductive efficiency ofVitex agnus-castus L is improved, and the culture medium and the method can meet the requirements of large-scale production.

Description

technical field [0001] The invention relates to a medium for tissue culture of Vitex paniculata and a rapid propagation method, and belongs to the technical field of plant tissue culture and propagation. Background technique [0002] Vitex agnus-castus L. is a plant of the genus Vitex Verbenaceae, an aromatic shrub with a flowering period of June to September. It is native to the Mediterranean region and is also cultivated in Jiangsu, Shanghai and other places in my country. Vitex agnus has beautiful flowers and is a rare seasonal flower in summer. Its large blue-purple inflorescences are rare in plant landscaping, and it is also widely used in landscaping. Vitex spica contains a variety of flavonoids, and its fruit has long been used to treat women's diseases. As a medicinal material, it has been included in the German Commission E botanical monograph. [0003] Vitex agnus is currently mainly propagated by sowing or cuttings, but the reproduction rate is low and the growth...

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Problems solved by technology

[0003] Vitex agnus is currently mainly propagated by sowing or cuttings, but the reproduction rate is low and the growth is slow, which cannot meet the needs of scientific research and large-scale production quickly and efficiently. Therefore, it is necessary to study a rapid propagation method of Vitex agnus

Tissue culture technology is one of the most effective methods for plant rapid propagation at present. It has the advantages of high reproduction coefficient, short seedling time and convenience for commercialization and industrial production. However, due to the browning, fungal pollution, glass However, there are few researches on the tissue culture rapid propagation technology of Vitex paniculata at present.

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