Culture method of cunninghamia lanceolata callus

A technology of callus and culture method, applied in the field of callus culture, to achieve the effects of firm texture, fast proliferation rate and high induction efficiency

Active Publication Date: 2015-01-21
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese fir tissue culture began in the 1950s. In the prior art, there are many studies on the rapid propagation of fir tissue culture by using the axillary bud induction method, and the use of explants to induce callus, and then induce adventitious buds through callus. There are relatively few studies on obtaining intact Chinese fir plants

Method used

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  • Culture method of cunninghamia lanceolata callus
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  • Culture method of cunninghamia lanceolata callus

Examples

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Effect test

Embodiment 1

[0024] A kind of Chinese fir callus culture method, choose the Chinese fir seed with full grain, no disease and insect damage as explant and put in the triangular flask, add the detergent solution that concentration is 1%~2%, seal the bottle mouth with gauze, put After shaking and rinsing on a shaker for 10 minutes, rinse under running water for 1 hour, then wash with sterile water twice, transfer the seeds to an ultra-clean workbench, sterilize with 0.15% mercury liter solution for 9 minutes, and wash with sterile water for 8 times Afterwards, the seeds were inoculated on 1 / 2 MS medium for culture to obtain sterile seedlings. After the seeds germinated, cut the leaves of 1-day-old Chinese fir seedlings and inoculate them with 1 / 2MS+1.0 mg / L 2,4-D+0.5mg / L KT+100 mg / L vitamin C+0.75 The induction culture was carried out in the induction medium composed of % agar + 3.0% sucrose. On the 2nd day, the leaves began to curl, and on the 3rd day, the wounds of the leaves or the edge o...

Embodiment 2

[0026] A kind of Chinese fir callus culture method, choose the Chinese fir seed with full grain, no disease and insect damage as explant and put in the triangular flask, add the detergent solution that concentration is 1%~2%, seal the bottle mouth with gauze, put After shaking and rinsing on a shaker for 15 minutes, rinse under running water for 1.5 hours, then wash with sterile water for 3 times, transfer the seeds to a clean bench, sterilize with 0.15% mercury liter solution for 10 minutes, and wash with sterile water for 8 minutes. After the second time, the seeds were inoculated on 3 / 4MS medium for cultivation to obtain sterile seedlings. After the seeds germinated, cut the leaves of Chinese fir seedlings with a seedling age of 5 days, and inoculated them with 1 / 2MS+2.0 mg / L 2,4-D+0.5mg / L KT+130 mg / L vitamin C+0.75 The induction culture was carried out in the induction medium composed of % agar + 3.0% sucrose. On the 3rd day, the leaves began to curl, and on the 5th day, ...

Embodiment 3

[0028] A kind of Chinese fir callus culture method, choose the Chinese fir seed with full grain, no disease and insect damage as explant and place in the triangular flask, add the detergent solution that concentration is 2%~3%, seal the bottle mouth with gauze, put After shaking and rinsing on a shaker for 12 minutes, rinse it under running water for 2 hours, then wash it twice with sterile water, transfer the seeds to an ultra-clean workbench and sterilize it with 0.15% mercury liter solution for 10 minutes, and wash it nine times with sterile water , the seeds were inoculated on MS medium for culture to obtain sterile seedlings. After the seeds germinated, cut the leaves of Chinese fir seedlings with a seedling age of 8 days, and inoculated them with 1 / 2MS+2.0 mg / L 2,4-D+1.0mg / L KT+150 mg / L vitamin C+0.75 The induction culture was carried out in the induction medium composed of % agar + 3.0% sucrose. On the 3rd day, the leaves began to curl, and on the 10th day, the wounds ...

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Abstract

The invention discloses a culture method of cunninghamia lanceolata callus. The culture method comprises the steps of: screening cunninghamia lanceolata seeds with filled particles and without pests as explants, carrying out sterile culture to obtain sterile seedlings, clipping leaves, stems or root systems of the sterile seedlings, inoculating in an induction culture medium for inducing callusogenesis, and finally transferring the callus formed by induction into a proliferation medium for proliferation. The culture method is simple and easy to operate, is capable of effectively shortening the induction and proliferation time of the callus and increasing the induction rate of the cunninghamia lanceolata callus, provides a large quantity of excellent callus materials with good colors and compact structures for the development of the cunninghamia lanceolata industry, and has a good economic benefit.

Description

technical field [0001] The invention relates to a callus culture method, in particular to a fir callus culture method. Background technique [0002] Plant callus culture is an important stage in the process of plant tissue culture. Callus can not only induce the formation of complete plants, but also be used for the preservation of germplasm resources, and it is also the source of cells and protoplasts for suspension culture. Chinese fir( Cunninghamia lanceolata ) is a unique coniferous timber tree species in my country. It has good material, fast growth and high yield. It is the main afforestation tree species in southern my country. Chinese fir tissue culture is mainly carried out through axillary bud induction or callus induction, and callus induction is an important link in the process of Chinese fir tissue culture. High induction rate and high-quality callus culture are the key to callus differentiation in the later stage. important basis. Chinese fir tissue culture ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄开勇陈琴郝海坤蓝肖戴俊唐庆兰张照远
Owner GUANGXI FORESTRY RES INST
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