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Tissue culture method of kudzu root

A technology of tissue culture and kudzu root, applied in the field of plant tissue culture, can solve problems such as death and kudzu browning

Active Publication Date: 2020-10-23
HUAIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, it is necessary to provide a method for kudzu tissue culture that can prevent kudzu from browning in order to solve the problem that kudzu is prone to browning and death during tissue culture

Method used

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  • Tissue culture method of kudzu root
  • Tissue culture method of kudzu root
  • Tissue culture method of kudzu root

Examples

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Embodiment 1

[0037] This embodiment provides a method for tissue culture of Pueraria lobata, which includes the following steps:

[0038] Explant treatment: Take the pink Pueraria lobata shoots with axillary buds and cut them into small sections of about 1 cm, each with axillary buds. The cut kudzu shoots were soaked in water with Tween for 30 minutes, and then rinsed with running water for 1 hour. After washing, disinfect with 75% alcohol for 20s, and then put in 0.1% HgCl 2 Soak and sterilize the solution for 6.5 minutes, then rinse with sterile water for 5 times, and finally absorb the water with sterile filter paper, and set aside.

[0039] Callus induction: Inoculate the treated shoots of Pueraria lobata on the first medium for culture, the induction temperature is 23℃~27℃, the relative humidity of the induction environment is 40%~50%, and the light treatment is alternately carried out for 12h With 12h dark treatment, the light intensity of the light treatment is 1500lux, the culture time...

Embodiment 2

[0046] This embodiment provides a method for tissue culture of Pueraria lobata, which includes the following steps:

[0047] Explant treatment: Take the pink Pueraria lobata shoots with axillary buds and cut them into small sections of about 1 cm, each with axillary buds. The cut kudzu shoots were soaked in water with Tween for 30 minutes, and then rinsed with running water for 1 hour. After washing, disinfect with 75% alcohol for 20s, then put in 0.1% HgCl 2 Soak in the solution for 6.5 minutes, then rinse with sterile water for 5 times, and finally absorb the water with sterile filter paper and set aside.

[0048] Callus induction: Inoculate the sterilized shoots of Pueraria lobata on the first medium for culture. The temperature of the culture room is 23℃~27℃, the relative humidity of the air is 40%~50%, and the 12h light treatment and 12h are alternately carried out. Dark treatment, the light intensity of the light treatment is 1500 lux, the culture time is 7 days, and the cal...

Embodiment 3

[0055] This embodiment provides a method for tissue culture of Pueraria lobata, which includes the following steps:

[0056] Explant treatment: Take the pink Pueraria lobata shoots with axillary buds and cut them into small sections about 1 cm, each with axillary buds. The cut kudzu shoots were soaked in water with Tween for 30 minutes, and then rinsed with running water for 1 hour. After washing, disinfect with 75% alcohol for 20s, and then put in 0.1% HgCl 2 Soak in the solution for 6 minutes, then rinse with sterile water for 5 times, and finally absorb the water with sterile filter paper and set aside.

[0057] Callus induction: Inoculate the sterilized shoots of Pueraria lobata on the first medium for culture, the temperature of the culture room is 23℃~27℃, the relative humidity of the air is 40%~50%, alternating 12h light treatment and 12h Dark treatment, the light intensity of the light treatment is 1700 lux, the cultivation time is 7 days, and the callus is obtained.

[005...

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Abstract

The invention relates to a tissue culture method of Pueraria thomsonii, comprising the steps of treating an explant, to be specific, collecting a Pueraria thomsonii twig with axillary buds, disinfecting, and killing bacteria for use; inducing a callus, to be specific, inducing the treated Pueraria thomsonii twig to a first medium for culturing to obtain the callus; inducing cluster buds, to be specific, dicing the callus, inoculating to a second medium, and culturing to obtain cluster buds; performing rooting culturing, to be specific, dividing the cluster buds to obtain single buds, transplanting the single buds to a third medium, and culturing to obtain Pueraria thomsonii plantlets. The tissue culture method of Pueraria thomsonii has the advantages that the Pueraria thomsonii twig with axillary buds having high differentiating capacity and low polyphenol content is used as an explant, different media are used in different culture stages, browning of Pueraria thomsonii during tissue culture is prevented, and survival rate of Pueraria thomsonii in tissue culture is effectively increased.

Description

Technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of Pueraria lobata. Background technique [0002] Pueraria lobata root has the functions of relieving the surface and refreshing the spleen, and is a commonly used medicinal material. Puerariathomsonii Benth, a leguminous plant, is called Pueraria thomsonii Benth because of its high starch content in its roots. Pueraria lobata has high medicinal and edible value. It can be used as a natural health food and has a large market demand. The artificial propagation of Pueraria lobata mainly adopts cutting propagation. This kind of breeding method needs to consume a large amount of the stems of Pueraria lobata, the reproduction coefficient is low, and the variety improvement is also difficult. Moreover, due to successive cuttings, the virus accumulates severely, which leads to the decline in yield and seed characteristics Deterioration and quality de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 贺安娜刘良科
Owner HUAIHUA UNIV
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