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Tissue culture propagation method of Polygonatum multiflorum

A Polygonatum multiflora, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of increased tissue culture cycle and cost, weak meristematic ability, easy pollution, etc., and achieve easy sterilization Effects of treatment, promotion of multiplication, and non-pollution

Active Publication Date: 2019-01-25
HUAIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using leaves, buds and tubers as explants is very easy to pollute, which greatly increases the cycle and cost of tissue culture; because the seeds contain a lot of nutrients such as starch, their meristematic ability is not strong. The process of wound tissue induction is still relatively difficult. The seed tissue culture reported in the past only germinates the seeds on the medium, and it is not a tissue culture method that undergoes dedifferentiation and redifferentiation in the true sense.

Method used

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  • Tissue culture propagation method of Polygonatum multiflorum
  • Tissue culture propagation method of Polygonatum multiflorum
  • Tissue culture propagation method of Polygonatum multiflorum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Prepare seed embryos: Collect mature Polygonatum multiflora fruits in mid-September, pile them up for 1 week, and wash the pulp with clean water after the peel is slightly rotten. Seeds were soaked in water for 24 hours, during which the water was changed 4 times. Rinse the seeds with running water for 5 minutes, then rinse with 0.1% HgCl 2 Soak for 5-8 minutes, rinse with sterile water, dry the water on the surface of the seeds with sterile filter paper, cut the treated seeds with dissecting scissors, and take out the seed embryos.

[0045] 2. Sterilization of seed embryos: use 0.1% HgCl 2 Soak the seed embryos for 8 minutes, and finally rinse them with sterile water for 8 times.

[0046] 3. Seed embryo inoculation and callus induction: The sterilized seed embryos are inoculated on the prepared medium. The formula of the medium is: MS+6-BA2.0mg / L+NAA0.2mg / L, per bottle Inoculate 4 to 5 plants. Put it on a dark culture rack with a temperature of 23°C-25°C and a r...

Embodiment 2

[0052] 1. Prepare the seed embryo: collect the mature fruit of Polygonatum multiflora in early October, pile it up for 1 week, and wash the pulp with clean water after the peel is slightly rotten. Seeds were soaked in water for 24 hours, during which the water was changed 4 times. Rinse the seeds with running water for 5 minutes, then rinse with 0.1% HgCl 2 Soak for 8 minutes, rinse with sterile water, dry the surface moisture of the seeds with sterile filter paper, cut the treated seeds with dissecting scissors, and take out the seed embryos.

[0053] 2. Sterilization of seed embryos: use 0.1% HgCl 2 Soak the seed embryos for 8 minutes, and finally rinse them with sterile water for 8 times.

[0054] 3. Seed embryo inoculation and callus induction: the sterilized seed embryos are inoculated on the prepared medium. The formula of the medium is: MS+6-BA 1.5mg / L+NAA 0.15mg / L, per bottle Inoculate 4 to 5 plants. Place it on a dark culture rack with a temperature of 23°C-25°C a...

Embodiment 3

[0059] 1. Prepare seed embryos: Collect mature Polygonatum multiflora fruits in late September, pile them up for 1 week, and wash the pulp with clean water after the peel is slightly rotten. Seeds were soaked in water for 24 hours, during which the water was changed 4 times. Rinse the seeds with running water for 5 minutes, then rinse with 0.1% HgCl 2 Soak for 5-8 minutes, rinse with sterile water, dry the water on the surface of the seeds with sterile filter paper, cut the treated seeds with dissecting scissors, and take out the seed embryos.

[0060] 2. Sterilization of seed embryos: use 0.1% HgCl 2 Soak the seed embryos for 6-7 minutes, and finally rinse them with sterile water for 8 times.

[0061] 3. Seed embryo inoculation and callus induction: the sterilized seed embryos are inoculated on the prepared medium, the formula of the medium is: MS+6-BA 2.2mg / L+NAA 0.2mg / L, per bottle Inoculate 4 to 5 plants. Put it on a dark culture rack with a temperature of 23°C-25°C an...

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Abstract

The invention relates to a tissue culture propagation method of Polygonatum multiflorum, comprising the steps of in an aseptic environment, collecting embryo from mature seeds of Polygonatum multiflorum by peeling, and sterilizing the embryo; inoculating the sterilized embryo to a callus induction medium, and performing light-free culture for 7-12 days so that the embryo expands to form a callus;cutting the callus into small pieces, inoculating the pieces to a callus subculture medium, and culturing for 18-25 days under light intensity of 800-1000 lux and daily lighting time of 10-12 h; cutting the callus subjected to subculture in step S3 into small pieces, inoculating the pieces to a sprouting medium, and culturing for 28-35 days under the light intensity of 1200-1800 lux and daily lighting time of 10-12 h, and inducing to obtain cluster buds; inoculating the robust single ones of the cluster buds to a rooting medium, and performing rooting culturing. The method herein helps greatlyreduce pollution rate of a tissue culture process, and improve propagation efficiency and seedling quality.

Description

technical field [0001] The invention relates to the field of seedling cultivation of medicinal plants, in particular to a tissue culture propagation method of Polygonatum polyflora. Background technique [0002] Polygonatum cyrtonema (Polygonatum cyrtonema) is a perennial herb of the genus Polygonatum in the family Liliaceae. Its rhizome is thick and thick. It is one of the base plants of Polygonatum medicinal materials recorded in the 2015 edition of "Chinese Pharmacopoeia". Lung, kidney and other effects. Polygonatum multiflora has a good taste and is both medicinal and edible. Recent studies have found that Polygonatum multiflora is also effective in the treatment of malignant tumors. Therefore, the market demand for this medicinal material is increasing day by day, and the planting area is also increasing year by year. [0003] The cultivation and management of Polygonatum multiflora is extensive, and it is often introduced and cultivated in the form of wild varieties. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 贺安娜
Owner HUAIHUA UNIV
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