A fast and efficient method to obtain the callus of multi-floral fireball

A callus and fireball technology, which is applied in the field of plant tissue culture, can solve the problems of limited reproduction number, inability to meet social production, danger of entering wild populations, etc., and achieve the effects of large dedifferentiation potential and strong tissue meristem ability.

Active Publication Date: 2022-05-13
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] There is a small amount of introduced and cultivated species in our country, but it is far from meeting the actual needs of social production, and a large number of multiplication is particularly important.
At present, the propagation methods commonly used in the world for this species are seed propagation and division (ball) propagation, but the number of reproduction of these two propagation methods is extremely limited, and it is inevitable to dig a large number of wild plants, and the wild population may enter danger. status

Method used

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  • A fast and efficient method to obtain the callus of multi-floral fireball
  • A fast and efficient method to obtain the callus of multi-floral fireball
  • A fast and efficient method to obtain the callus of multi-floral fireball

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Embodiment Construction

[0021] Grab the underground part of an adult plant of Scadoxus multiflorus subsp. multiflorus in 3-4 years, and select the branches (lateral rhizomes) that grow from the mother plant but do not emerge from the ground, such as figure 1 shown. Select the stem segment with a length of 3-8cm at the top of the branch, such as figure 1 Wash as indicated by the yellow arrow. Then remove the about 1cm long stem segment apex of the top, and the remaining part is cut into 0.5-0.8cm long segments to obtain explants, which are the lateral rhizomes described in Table 1. At the same time, get the leaf tissue (including leaf tip, leaf margin and leaf vein), cut into 0.5-0.8cm long small cubes, as the explant of leaf; get the middle part of the pseudostem, cut into 0.5-0.8cm long small Cube, as explant of pseudostem; take 1-3cm of root tip, cut into 0.5-0.8cm long section, as explant of root; strip off the outer layer of corm that has been sterilized, leaving only the "core" Part, cut this...

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Abstract

The invention discloses a method for obtaining multi-floral fireballs quickly and efficiently ( Scadoxus multiflorus subsp. multiflorus ) method of callus, specifically as follows: grab the corm of 3-4 years old multi-flowered fireball flower, select the stem section of 3-8cm from the top of the lateral rhizome and wash it, remove the 0.5-1cm long tip of the top stem section, and cut the remaining part Cut into 0.5-0.8cm-long sections as explants, and culture them in callus induction medium MS+2.4-D 0.45-0.48 mg / L+NAA 0.05-0.08 mg / L to obtain callus. Compared with division propagation and sowing propagation, the method has the advantages of not destroying the mother plant, producing many seedlings at one time, easy large-scale production, etc., and is suitable for mass propagation.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, in particular to a method for rapidly and efficiently obtaining callus of Scadoxus multiflorus subsp. multiflorus. Background technique [0002] Scadoxus multiflorus subsp. multiflorus is native to a wide area of ​​sub-Saharan Africa, from Senegal to Somalia, and from Ethiopia to South Africa. [0003] It is a perennial herb, 15-120cm tall, bulbous, with petioles forming pseudostems 5-60cm high. The leaves are lanceolate to obovate, acute to acuminate or obtuse, the base of the leaf gradually narrows to nearly heart-shaped, and the size of the leaves varies greatly; flowers first and then leaves. Scapes 12-75cm tall, pseudobulbs and scapes usually have maroon to dark purple spots. Involucral bracts droop or wither early, lanceolate to linear, up to 6cm long, less than 1.5cm wide, colorless or slightly reddish. Inflorescence hemispherical to spherical, 10-200 flowers. The peduncle is 1-6.5c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 钟志祥王青锋严雪胡光万刘艳玲李洪林
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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