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Primary fir wood culture bud induction method

A technology of primary culture and bud induction, applied in the field of fir asexual reproduction, can solve problems such as vitrification and browning of explants, and achieve the effects of preventing browning, promoting bud induction, and good bud growth.

Active Publication Date: 2013-09-11
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of fir primary culture bud induction method, improve the existing bud induction technology, overcome the problems such as explant browning and vitrification in the bud induction process, improve bud induction rate, bud index and bud growth status, Obtain a large number of buds with good quality, so as to meet the needs of multiplication and culture, and promote the establishment of a rapid propagation system of Chinese fir tissue culture

Method used

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  • Primary fir wood culture bud induction method
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  • Primary fir wood culture bud induction method

Examples

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Embodiment 1

[0026] A method for inducing buds of Chinese fir primary culture. The bud induction medium used is modified 1 / 2 MS medium, supplemented with kinetin 6-furfurylaminopurine (KT), vitamin C, agar and sucrose; using 1 / 2 MS medium Basic medium, NH in macronutrients 4 NO 3 The content is 550 mg / L; the content of kinetin 6-furfurylaminopurine (KT) is 1.0 mg / L; the content of vitamin C is 10 mg / L; the content of agar is 0.8% and the content of sucrose is 3%, made into bud induction medium spare. Choose 4-6 cm long semi-lignified thick sprouts from the base of the fir as explants, cut off the sprout needles of fir, place them in a triangular flask, and add 1~2% detergent. Use gauze to seal the mouth of the bottle, place it on a shaker with a rotation speed of 200 r / min for 10 min, rinse under running water for 1 h, and then wash with sterile water twice to turn the explants into ultra-clean Sterilize with 0.15% mercury liter solution on the workbench for 9 min; among them, drop 1 drop ...

Embodiment 2

[0028] A method for inducing buds of Chinese fir primary culture. The bud induction medium used is modified 1 / 2MS medium, supplemented with kinetin 6-furfurylaminopurine (KT), vitamin C, agar and sucrose; the basic medium is 1 / 2MS medium Medium, NH in macronutrients 4 NO 3 The content is 550 mg / L; the content of kinetin 6-furfurylaminopurine (KT) is 1.5 mg / L; the content of vitamin C is 15 mg / L; the content of agar is 0.8% and the content of sucrose is 3%, made into bud induction medium spare. Choose 4~6cm long semi-lignified thick sprouts from the base of fir as explants, cut the sprout needles of fir, put them in a triangular flask, and add 2~3% detergent The solution, use gauze to seal the mouth of the bottle, place it on a shaker with a rotation speed of 200r / min for 15 minutes, rinse under running water for 1.5 hours, and then rinse with sterile water twice, then transfer the explants to the ultra-clean workbench Sterilize with 0.15% mercury liter solution for 9 min; amon...

Embodiment 3

[0030] A method for inducing buds of Chinese fir primary culture. The bud induction medium used is modified 2 / 3MS medium, supplemented with kinetin 6-furfurylaminopurine (KT), vitamin C, agar and sucrose; based on 2 / 3MS medium Medium, NH in macronutrients 4 NO 3 The content is 825 mg / L; the content of kinetin 6-furfurylaminopurine (KT) is 2.0 mg / L; the content of vitamin C is 15 mg / L; the content of agar is 0.8% and the content of sucrose is 3%, made into a bud induction medium spare. Choose the 6~8 cm long semi-lignified thick sprouting shoots from the base of the fir as explants, cut off the sprouting needles of the fir, place them in a triangular flask, and add 2~3% detergent. Use gauze to seal the mouth of the bottle, place it on a shaker with a rotation speed of 200r / min for 15 minutes, rinse under running water for 2 hours, then rinse with sterile water twice, and transfer the explants to a clean bench Sterilize with 0.15% mercury liter solution for 9 min; among them, dr...

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Abstract

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.

Description

Technical field [0001] The invention relates to a vegetative propagation technology of fir, in particular to a method for inducing the primary culture bud of fir. Background technique [0002] Chinese fir( Cunninghamia lanceolata ) Is a Chinese fir family, an important fast-growing timber species unique to my country. Cunninghamia lanceolata has wide adaptability, good material quality and wide range of uses. It is one of the main afforestation tree species in the south of China. With the development of my country’s economy and the country’s emphasis on forestry, the afforestation area of ​​fir will continue to expand, and the requirements for the quantity and quality of seedlings will continue to increase. Therefore, it is still difficult to meet the requirements of seedling cultivation of fir sowing, cutting, layering and grafting. Market demand. Tissue culture technology is a kind of rapid asexual reproduction technology. Selecting superior families or clones of Chinese fir a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黄开勇陈琴郝海坤戴俊张照远蓝肖唐庆兰
Owner GUANGXI FORESTRY RES INST
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