Primary fir wood culture bud induction method

A technology of primary culture and bud induction, applied in the field of fir asexual reproduction, can solve problems such as vitrification and browning of explants, and achieve the effects of preventing browning, promoting bud induction, and good bud growth.
CN103283598AActive Publication Date: 2013-09-11GUANGXI FORESTRY RES INST

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGXI FORESTRY RES INST
Publication Date
2013-09-11

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Abstract

The invention discloses a primary fir wood culture bud induction method. The method comprises the following steps: inoculating an explant as a coppice shoot of a fir wood base part and subjected to sterile explant treatment to a bud induction culture medium subjected to high-quality primary culture, culturing under the conditions of proper temperature, humidity and illumination intensity, and inducing bud germination. The conventional fir wood induction technology is improved, the problems that the explant is subjected to browning and vitrifaction in the fir wood bud induction process and the like are solved, the fir wood bud induction rate, the budding index and the germination and growth conditions are improved, and large-number high-quality buds are obtained, so that the multiplication culture requirement is met, the establishment of a rapid fir wood tissue culture reproductive system is promoted, and the method has high economic benefits and social benefits.
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Description

Technical field

[0001] The invention relates to a vegetative propagation technology of fir, in particular to a method for inducing the primary culture bud of fir. Background technique

[0002] Chinese fir( Cunninghamia lanceolata ) Is a Chinese fir family, an important fast-growing timber species unique to my country. Cunninghamia lanceolata has wide adaptability, good material quality and wide range of uses. It is one of the main afforestation tree species in the south of China. With the development of my country’s economy and the country’s emphasis on forestry, the afforestation area of ​​fir will continue to expand, and the requirements for the quantity and quality of seedlings will continue to increase. Therefore, it is still difficult to meet the requirements of seedling cultivation of fir sowing, cutting, layering and grafting. Market demand. Tissue culture technology is a kind of rapid asexual reproduction technology. Selecting superior families or clones of Chinese fir a...

Claims

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