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Medium for tissue culture of populus wulianensis

A tissue culture and culture medium technology, applied in the field of plant tissue culture, can solve problems such as easy vitrification, and achieve the effects of reducing the vitrification rate, improving the survival rate, and simple proportioning

Active Publication Date: 2017-08-15
SHANDONG FOREST GERMPLASM RESOURCES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the conventional tissue culture process of Populus plants, especially in the tissue culture of poplar plants, vitrification is very easy to occur

Method used

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  • Medium for tissue culture of populus wulianensis
  • Medium for tissue culture of populus wulianensis
  • Medium for tissue culture of populus wulianensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A culture medium for Populus wulian tissue culture comprises a primary culture medium, a devitrification medium, a proliferation medium and a rooting medium. After culturing in devitrification medium, the degree of vitrification of Populus wulian tissue culture can be greatly reduced. The primary culture medium is MS medium with 25g / L sucrose and 5.5g / L agar powder added on the basis of MS medium.

[0020] Divide the stem sections of robust test-tube bushy seedlings into 8 groups, with 48 stem sections in each group, and inoculate them on average in the MS basal medium of 0.5mg / L 6-BA+0.05mg / L NAA and add 25g / L sucrose, 5.5g / L Agar powder in MS medium. The test results are shown in Table 1.

[0021] Table 1 The results of the primary culture medium test

[0022]

[0023] The first-generation culture medium used in this example is based on the MS culture medium, with the addition of 25 g / L sucrose and 5.5 g / L agar powder.

[0024] The devitrification medium is 1 / 2...

Embodiment 2

[0057] A culture medium for Populus wulian tissue culture comprises a primary culture medium, a devitrification medium, a proliferation medium and a rooting medium. The primary culture medium is MS medium with 25g / L sucrose and 5.5g / L agar powder added on the basis of MS medium.

[0058] Divide the stem sections of robust test-tube bushy seedlings into 8 groups, with 48 stem sections in each group, and inoculate them on average in the MS basal medium of 0.5mg / L 6-BA+0.05mg / L NAA and add 25g / L sucrose, 5.5g / L Agar powder in MS medium. The test results are shown in Table 10.

[0059] Table 10 The first generation culture medium test result

[0060]

[0061] The first-generation culture medium used in this example is based on the MS culture medium, with the addition of 25 g / L sucrose and 5.5 g / L agar powder.

[0062] The devitrification medium is 1 / 2 (N) MS medium, and 0.05mg / L NAA, 0.4mg / L 6-BA, 30g / L sucrose, 6.5g / L agar powder are added to reduce the N source is benefic...

Embodiment 3

[0094] A culture medium for Populus wulian tissue culture comprises a primary culture medium, a devitrification medium, a proliferation medium and a rooting medium. The primary culture medium is MS medium with 25g / L sucrose and 5.5g / L agar powder added on the basis of MS medium.

[0095] Divide the stem sections of robust test-tube bushy seedlings into 8 groups, with 48 stem sections in each group, and inoculate them on average in the MS basal medium of 0.5mg / L 6-BA+0.05mg / L NAA and add 25g / L sucrose, 5.5g / L Agar powder in MS medium. The test results are shown in Table 19.

[0096] Table 19 Primary medium test results

[0097]

[0098] The first-generation culture medium used in this example is based on the MS culture medium, with the addition of 25 g / L sucrose and 5.5 g / L agar powder.

[0099] The devitrification medium is 1 / 2 (N) MS medium, and 0.05mg / L NAA, 0.4mg / L 6-BA, 30g / L sucrose, 6.5g / L agar powder are added to reduce the N source is beneficial to reduce the in...

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Abstract

The invention relates to the field of plant tissue culture, in particular to a medium for tissue culture of populus wulianensis. The medium for tissue culture of the populus wulianensis comprises a primary medium, a de-vitrification medium, a proliferation medium and a rooting medium; the primary medium is an MS medium with addition of 25g / L of sucrose and 5.5g / L of agar powder; the de-vitrification medium is a 1 / 2 (N) MS medium with addition of 0.05mg / L of NAA, 0.4mg / L 6-BA, 30g / L of the sucrose and 6.5g / L of the agar powder; the proliferation medium is the MS medium with addition of 0.03mg / L of the NAA, 0.4-0.5mg / L of the 6-BA, 30g / L of the sucrose and 6.5g / L of the agar powder; and the rooting medium is the 1 / 2 MS medium with addition of 0.05mg / L of the NAA, 15g / L of the sucrose and 6.5g / L of the agar powder. The medium adopted by the invention is simple to operate, remarkably lowers the vitrification occurrence rate in the populus wulianensis tissue culture process and increases the tissue culture propagation rate of the populus wulianensis.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, in particular to a medium for tissue culture of Populus wulian. Background technique [0002] Wulian poplar (Populus wulianensis) belongs to Salicaceae (Salicaceae), Populus (Populus) poplar school, difficult to root tree species, also known as Kunyu poplar, is a rare tree species unique to Shandong, mainly distributed in Shandong Rizhao (Wulian Mountain) and In Yantai (Kunyu Mountain, Zhaohu Mountain) and other places, it grows in miscellaneous forests in ravines at an altitude of 300-500m, scattered or forming forests. [0003] Asexual reproduction technology plays an important role in the sustainable development of modern forestry. In the popularization and application of hybrid varieties, it is of great significance to maintain their genetic stability by means of asexual reproduction. However, the disadvantages of asexual reproduction techniques such as grafting and cuttings are low repro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 王艳咸洋韩义解孝满李文清鲁仪增赵永军赵立军张学勇
Owner SHANDONG FOREST GERMPLASM RESOURCES CENT
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