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Healthcare medicinal material Maca tissue cultured seedling vitrification control method

A technology of maca grouping and vitrification, which is applied in the field of plant tissue culture, can solve the problems of sickness, reduction of seedling quality and proliferation rate, and deformity of tissue culture seedlings, and achieve the effect of simple method, reduction of vitrification phenomenon, and improvement of proliferation rate

Inactive Publication Date: 2015-12-30
大理白族自治州农业科学推广研究院药用植物及农业新技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Vitrified seedlings are prone to appear during the cultivation of maca tissue culture seedlings, resulting in deformity and morbidity of tissue culture seedlings, reducing the quality and proliferation rate of seedlings

Method used

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  • Healthcare medicinal material Maca tissue cultured seedling vitrification control method
  • Healthcare medicinal material Maca tissue cultured seedling vitrification control method
  • Healthcare medicinal material Maca tissue cultured seedling vitrification control method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take MS medium, add 20g of sucrose and 8g of agar per liter, and adjust pH=6.2 at the same time, as the basic medium, take 1 / 2MS medium, add 20g of sucrose and 8g of agar per liter, and adjust pH=6.2 at the same time, as 1 / 2MS medium; take the plump maca seeds, rinse them with water, soak them in sterile water for 8 hours, disinfect them with 75% alcohol for 45 seconds, transfer them to 1% mercuric chloride for disinfection for 8 minutes, and rinse them with sterile water for 6 minutes. all over. Inoculate in MS medium, culture temperature is 20°C, light intensity is 1500Lx, light time is 10 hours, culture for 18 days, until 4 true leaves of seedlings grow out, get sterile seedlings;

[0020] Add 0.5 mg of NAA and 2.0 mg of 6-BA per liter to the basic MS medium, cut 4 pieces of sterile seedlings with growth points from true leaves on the ultra-clean workbench and inoculate them into the medium. Insert the lower end of the stem segment into the culture medium, the depth...

Embodiment 2

[0024] Take MS medium, add 30g of sucrose and 7g of agar per liter, and adjust pH=6.0 at the same time, as the basic medium, take 1 / 2MS medium, add 30g of sucrose and 7g of agar per liter, and adjust pH=6.0 at the same time, as 1 / 2MS medium; take plump maca seeds, rinse them with water, soak them in sterile water for 7 hours, sterilize them with 75% alcohol for 30 seconds, sterilize them in 0.1% mercuric chloride for 12 minutes, and then rinse them with sterile water 5 times. Inoculate in MS medium, culture temperature is 20°C, light intensity is 1500Lx, light time is 10 hours, culture for 18 days, until 4 true leaves of seedlings grow out, get sterile seedlings;

[0025] Add 0.2 mg of NAA and 1.0 mg of 6-BA per liter to the basic MS medium, cut 4 pieces of sterile seedlings with growth points from true leaves on the ultra-clean workbench and inoculate them into the medium. Insert the lower end of the stem segment into the culture medium, the depth is 0.4 cm, the daytime cult...

Embodiment 3

[0029] Take MS medium, add 40g of sucrose and 6g of agar per liter, and adjust pH=5.8 at the same time as the basic medium, take 1 / 2MS medium, add 40g of sucrose and 6g of agar per liter, and adjust pH=5.8 at the same time, as 1 / 2MS medium; take plump maca seeds, rinse them with water, soak them in sterile water for 6 hours, sterilize them with 75% alcohol for 30 seconds, sterilize them in 0.5% mercuric chloride for 10 minutes, and then rinse them with sterile water 4 times. Inoculate in MS medium, culture temperature is 20°C, light intensity is 1500Lx, light time is 10 hours, culture for 18 days, until 4 true leaves of seedlings grow out, get sterile seedlings;

[0030] Add 0.3 mg of NAA and 1.5 mg of 6-BA per liter to the basic MS medium, cut 4 pieces of sterile seedlings with growth points from true leaves on the ultra-clean workbench and inoculate them into the medium. Insert the lower end of the stem segment into the culture medium, the depth is 0.5 cm, the daytime cultu...

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Abstract

The invention discloses a healthcare medicinal material Maca tissue cultured seedling vitrification control method. An Maca tissue cultured seedling vitrification phenomenon is effectively controlled by preferably selecting proliferation medium, improving culture conditions, adopting MS and 1 / 2 MS as the culture medium during Maca tissue culture, adding NNA and 6-BA with different concentration and changing dosage of sucrose and agar and the culture conditions. The method comprises the following steps: 1) establishing Maca aseptic seedlings; 2) inducing calluses; 3) proliferating cluster buds; 4) strengthening seedlings and rooting. By preferably selecting the concentration of hormone, the optimum concentration ratio of the culture medium to the hormone suitable for Maca proliferation is obtained, the proliferation rate of Maca tissue cultured seedlings is generally improved, a strong guarantee is provided for the normal and healthy growth of the tissue cultured seedlings and the Maca tissue cultured seedling vitrification ratio tends to 0.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for vitrification control of tissue cultured seedlings of Maca, a health-care medicinal material. Background technique [0002] Maca is mainly produced in the Andean Plateau at an altitude of 3,500-4,000 meters in Peru, South America. It is an annual herb belonging to the genus Brassicaceae. It is a rare alpine medicinal and edible plant. It is not only rich in protein and essential amino acids , multiminerals (K, Ca, Fe, Zn) and multivitamins (VB 1 , VB 2 , VC) and other nutrients, and contains secondary metabolic active ingredients such as maca alkaloids, glucosinolates and sterols. A large number of studies have shown that Maca has the effects of improving fertility, improving sexual function, regulating endocrine, enhancing immunity, anti-oxidation, anti-stress, anti-fatigue and anti-cancer, and has effects on rheumatism, respiratory diseases, depressi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张金莲段忠朱秀梅张顺仁彭智娥王林鹏解明坤段彦君张梅芳苏碧玉杨光明李顺堂于世龙
Owner 大理白族自治州农业科学推广研究院药用植物及农业新技术研究所
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