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Paraserianthes falcataria clone tissue culture method

A technique of tissue culture and clonal lines, applied in the biological field, can solve the problems of difficulty in collecting explants, hindering large-scale reproduction, and different growth speeds of Jacaranda stands

Active Publication Date: 2016-04-27
GUANGDONG ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivated area of ​​Jacaranda jacaranda in my country has reached more than 30,000 hectares, but the existing cultivated forests are all seedling afforestation, which has the following defects: (1) Jacaranda jacaranda seeds mainly rely on imports and a small number of scattered mother trees that were introduced early in the country. Collecting, seed sources are unclear, quality is uneven, and the yield year fluctuates greatly; (2) Due to the high degree of heterozygosity of forest tree genes, there are generally differentiation problems such as different growth speeds, different numbers and sizes of branches in Jacaranda stands
[0003] At present, the tissue culture technology of Jacaranda jacaranda is still in the experimental research stage, mainly due to bottlenecks such as difficulty in explant collection, high difficulty in sterilization, serious vitrification and yellowing, and low multiplication factor, which hinder its large-scale reproduction

Method used

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  • Paraserianthes falcataria clone tissue culture method
  • Paraserianthes falcataria clone tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The method for the tissue culture of the jacaranda clonal line of embodiment 1

[0025] The method for tissue culture of the jacaranda jacaranda clone of the present embodiment comprises the following steps:

[0026] 1. Acquisition of explants: In the provenance and family test forest of Jacaranda jacaranda introduced by the Institute of Forestry Science, Boluo County, Guangdong Province, BLS16 seeds of 17-year-old excellent mother trees were collected, and sterile explants were obtained through sterile seedling cultivation techniques body.

[0027] 2. Induction culture: Select a healthy sterile seedling with a seedling height of about 3.0cm. After cutting off the root, cut it into two sections, one section contains 1 terminal bud, and the other section contains 2 axillary buds, and insert it into induction culture. After 35 days of culture, the aging callus at the base was excised to continue transfer, and cultured for another 30 days. The composition of the inductio...

Embodiment 2

[0032] The influence of the component concentration of embodiment 2 proliferation medium on proliferation culture

[0033]The method for tissue culture of the jacaranda clonal line of this embodiment is the same as that of Example 1 except for the concentration of some components of the proliferation medium in step 2. The difference between the proliferation medium of this example and Example 1 is shown in Table 1 Shown:

[0034] Concentrations of 6-BA, NAA, and sucrose in the proliferation medium in Table 1

[0035] Test No.

6-BA(mg / L)

NAA(mg / L)

Sucrose (g / L)

Example 2a

0.1

0.01

25

Example 2b

0.15

0.03

30

Example 1

0.2

0.05

30

Example 2c

0.3

0

25

Example 2d

0.4

0.02

25

Example 2e

0.5

0.04

30

[0036] After 30 days of natural scattered light culture and 20 days of artificial light culture, the effects of different 6-BA and NAA concentra...

Embodiment 4

[0043] The impact of embodiment 4 seedling hardening on the survival rate of seedlings

[0044] The method for tissue culture of the jacaranda jacaranda clone of the present embodiment comprises the following steps:

[0045] 1. Obtain explants: the same as step 1 in Example 1;

[0046] 2. Induction culture: same as step 2 of Example 1;

[0047] 3. Proliferation culture: with step 3 of Example 1;

[0048] 4. Rooting culture: when the adventitious buds grow to 3-5 cm, they are cut off and transferred to the rooting medium, and cultivated for 35 days. The resulting plants are weak and easily wilted in the wind, and the root systems are of different lengths. Rooting medium composition is: 1 / 2MS medium + NAA (0.2mg / L) + indole butyric acid (IBA, 0.2mg / L) + sucrose (25g / L) + carrageenan (7g / L), pH value 5.8. The culture temperature is about 28°C.

[0049] 5, plant transplanting: with the step 6 of embodiment 1. The seedling rate is 60%.

[0050] It can be seen that the seedl...

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Abstract

The invention relates to a paraserianthes falcataria clone tissue culture method. According to the method, aseptic seedlings obtained from paraserianthes falcataria excellent mother tree seeds with an aseptic seedling-raising technology are adopted as explants, or paraserianthes falcataria excellent mother tree budding shoots are adopted as explants; the explants are subjected to induction culture, proliferation culture, rooting culture, and seedling hardening, and are transplanted into and cultured in a seedling-raising medium. According to the method, technical links such as the induced proliferation process, culture media and culture conditions, and seedling hardening are optimized. Cytokinin 6-BA is added into an induction medium; during proliferation culture, tissue culture seedlings are subjected to natural scattering light culture for 28-35 days, such that rooting and seedling hardening are combined; plant transplanting is carried out at later than 4:30pm on sunny days or on cloudy days; and the components of various culture media are optimized, such that seedling vitrification phenomenon, yellowing phenomenon and ineffective callus generation are effectively reduced, proliferation rate and seedling emergence rate are improved, a culture period is shortened, and seedling survival rate can be higher than 90%.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for culturing jacaranda clones. Background technique [0002] Paraserianthes falcataria is an evergreen tree species belonging to the Mimosaceae family. It can become a forest in 3 to 4 years after planting, and can be used in 4 to 5 years. The root system has the ability to form nodules and fix nitrogen, which can improve soil and improve soil fertility. , is an eco-friendly and excellent fast-growing industrial timber tree species. The cultivated area of ​​Jacaranda jacaranda in my country has reached more than 30,000 hectares, but the existing cultivated forests are all seedling afforestation, which has the following defects: (1) Jacaranda jacaranda seeds mainly rely on imports and a small number of scattered mother trees that were introduced early in the country. Collection, seed sources are unclear, good and bad quality are uneven, and the yield year fluctuates greatly;...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 晏姝韦如萍胡德活王润辉郑会全
Owner GUANGDONG ACAD OF FORESTRY
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