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Tissue culture rapid propagation method for rieger begonia

A technology of lattice crabapple and crabapple, which is applied in the field of tissue culture and rapid propagation of Rieger crabapple, can solve the problems of difficult batch seedlings for popularization and application, lack of provenance, difficulty in cutting, etc., and achieve the effect of reducing the risk of vitrification and browning

Inactive Publication Date: 2021-01-29
SHANXI ACAD OF AGRI SCI GARDENING RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to many factors such as lack of provenance, difficult rooting of cuttings, and genetic variation, it is difficult to obtain batches of seedlings for popularization and application in a short period of time through conventional propagation methods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A Rieger crabapple tissue culture rapid propagation method comprises the following steps:

[0018] 1) Treatment and disinfection of explants: Select the tops of new shoots of robust Rieger crabapple plants as explants, first soak and clean them with detergent powder water for 10 minutes, then rinse them under running water for 3 hours and place them in an ultra-clean workbench, first use 75% After disinfecting with ethanol for 30 seconds, wash with sterile water for 5 times, then disinfect with 0.2% mercuric chloride solution for 15 minutes, rinse with sterile water for 6 times, and then dry the water droplets on the surface with sterile filter paper before use;

[0019] 2) Induction culture: Cut the Rieger crabapple explants treated in step 1 into small pieces of 0.5-1 cm and inoculate them on the induction medium, and culture them at 25°C, under the condition of light for 12 hours, and light intensity of 1400lx until induction Clustered buds are formed; the induction ...

Embodiment 2

[0024] A Rieger crabapple tissue culture rapid propagation method comprises the following steps:

[0025] 1) Treatment and disinfection of explants: Select the tops of new shoots of robust Rieger Begonia plants as explants, first soak and clean them with detergent powder water for 5 minutes, then rinse them under running water for 1 hour and place them in an ultra-clean workbench, first use 75% After disinfecting with ethanol for 5 seconds, wash with sterile water for 3 times, then disinfect with 0.2% mercuric solution for 5 minutes, rinse with sterile water for 4 times, and then dry the water droplets on the surface with sterile filter paper before use;

[0026] 2) Induction culture: Cut the Rieger crabapple explants treated in step 1 into small pieces of 0.5-1 cm and inoculate them on the induction medium, and culture them at 18°C, under the condition of light for 14 hours, and light intensity of 1400lx until induction Clustered buds are formed; the induction medium is: WPM+...

Embodiment 3

[0031] A Rieger crabapple tissue culture rapid propagation method comprises the following steps:

[0032] 1) Treatment and disinfection of explants: Select the tops of new shoots of robust Rieger crabapple plants as explants, first soak and clean them with detergent powder water for 8 minutes, then rinse them under running water for 1.5 hours and place them in an ultra-clean workbench. % ethanol for 25 seconds, washed with sterile water for 4 times, then disinfected with 0.2% mercuric solution for 12 minutes, rinsed with sterile water for 5 times, and then wiped dry the water droplets on the surface with sterile filter paper before use;

[0033] 2) Induction culture: Cut the Rieger crabapple explants treated in step 1 into small pieces of 0.5-1 cm and inoculate them on the induction medium, culture them at 20°C, light for 13 hours, and a light intensity of 1400 lx until the induction is formed Clustered buds; induction medium: WPM+2mg / L 6-BA, 0.5mg / L NAA, 20g / L sucrose, 5g / L a...

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PUM

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a tissue culture rapid propagation method for rieger begonia. According to the method, the top end ofa single bud of the rieger begonia serves as an explant, and a rieger begonia tissue culture rapid propagation technical system is established through the steps of explant disinfection, bud primary induction culture, subculture, rooting culture, seedling hardening, transplanting and the like, so that the purpose of rapid propagation of the rieger begonia is achieved. According to the method, low-temperature induction and subculture are adopted, risks of vitrification and browning of culture seedlings can be reduced, and the method is widely applied to the technical field of tissue culture rapid propagation of malus spectabilis.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method of Rieger crabapple tissue culture. Background technique [0002] Rieger Begonia is a herbaceous plant belonging to Cucurbitaceae, Begonia genus, and Begoniaceae. Rieger Begonia is a fibrous root system, with plump plant shape, alternate single leaves, asymmetrical heart shape, mostly emerald green leaves, rarely reddish brown. [0003] The flower shapes are various, most of which are double petals, and the flowers are rich in red, orange, yellow, white, etc. The flowers are huge and colorful, with unique appearance, color and fragrance; and the flowering period is long, which can last from December to April in the next spring . The blooming period is from April to June and from September to December. It likes warm, humid and semi-shade environment. Distributed in tropical and subtropical regions. Suitable for ornamental sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/28A01G24/15
CPCA01G31/00A01H4/001A01H4/008A01G24/15A01G24/28
Inventor 张超贾民隆武江梁峥段九菊宋卓琴
Owner SHANXI ACAD OF AGRI SCI GARDENING RES INST
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