Industrialized seedling culture method for detoxified health sugarcane seedlings

Abstract

The invention discloses an industrialized seedling culture method for detoxified health sugarcane seedlings. According to the industrialized seedling culture method, a sealing cover with ventilating and bacteria filtering functions is utilized for replacing conventional closed covers to seal a culture container in each indoor seedling culture stage of the detoxified health sugarcane seedlings; and by virtue of the ventilating sealing cover, the culture container has certain permeability, so that culture conditions from the establishing of sterile explants to rooting and acclimatization stages are regulated and controlled, the photoautotrophical capacity of cultures is remarkably improved, and the quality and the seedling culture efficiency of tissue cultured seedlings are improved. Practices show that by virtue of the industrialized seedling culture method, the vitrification rate of explants can be decreased in an induction stage of the explants; in multiplication and expanding propagation stages, the proportion of vitreous buds can be reduced, and the quality of cultured seedlings is improved; in a rooting stage, the induced rooting time can be shortened, and the rooting rate is increased; and in acclimatization and transplant stages, the seedling domestication period is shortened, and the survival rate of transplanted seedlings is increased. Therefore, the industrialized production efficiency of the detoxified health sugarcane seedlings is finally integrally improved, and the production cost of cultured seedlings is lowered.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of healthy sugarcane seedlings, and in particular relates to an industrial seedling raising method for healthy sugarcane seedlings. Background technique [0002] Sugarcane virus-free healthy seedlings generally refer to sugarcane seedlings that do not carry the pathogens of sugarcane perennial dwarf disease and sugarcane mosaic disease (hereinafter referred to as pathogens) obtained by means of biotechnology. The conventional technical process is as follows: pathogen removal →Establishment of sterile explants→Induction of clustered buds→Pathogen detection→Proliferation and rapid propagation of healthy seedlings→Rooting culture→Pseudo-planting on sand beds in the first-level nursery→Pseudo-planting in nutrient cups in the second-level nursery→Field expansion of first-class seedlings in field Propagation→Pathogen detection→Qualified seedling field expansion of second-level seedling field→P...

AI Technical Summary

Problems solved by technology

Usually, when the healthy seedlings of sugarcane are detoxified, the seedlings are cultivated in sealed containers and rely on the sucrose in the medium to provide the carbon source and energy needed for growth. It is: in the stage of external establishment, cluster bud induction and proliferation and rapid propagation, culture vitrification and glass seedling phenomenon are easy to occur; in the rooting stage, the rooting rate is low, the rooting is slow and the root quality is not high; in the transplanting stage, the hardening and domestication stage Long, low survival rate, etc., these all lead to low overall seedling rate, high production cost and other problems

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