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A method for improving the proliferation and differentiation efficiency of Orchid rhizomes

A rhizome and Chinese orchid root technology, which is applied in plant tissue culture to improve the proliferation and differentiation efficiency of Chinese orchid rhizomes, and can solve the problems of inability to realize industrialized production of seedlings and low reproductive efficiency

Active Publication Date: 2021-05-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the low efficiency of existing Chinese orchid industrial reproduction and the defects or deficiencies that cannot realize the industrial production of seedlings, and provide a method for improving the efficiency of Chinese orchid rhizome proliferation and differentiation

Method used

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  • A method for improving the proliferation and differentiation efficiency of Orchid rhizomes
  • A method for improving the proliferation and differentiation efficiency of Orchid rhizomes
  • A method for improving the proliferation and differentiation efficiency of Orchid rhizomes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Addition of copper sulfate in the culture medium of Example 1 promotes the proliferation and differentiation of "Yuxianglan" rhizomes

[0039] In order to find ways to increase the efficiency of rhizome proliferation and differentiation of "Yuxianglan", the effect of copper sulfate on the proliferation and differentiation of "Yuxianglan" rhizomes was studied. The specific method is as follows:

[0040] S1. Culture medium design and preparation include the following steps:

[0041] S11. Culture medium design: the basic proliferation medium is MS+6-BA 1.0mg L -1 +NAA0.5mg·L -1 +AC0.5g·L -1 + sucrose 30.0g·L -1 + Carrageenan 7.0g·L -1 (pH=5.8±0.2), add 0, 2, 4 and 6mg·L to this medium respectively -1 Copper sulfate was used for proliferation test. The basic differentiation medium is MS+6-BA1.5mg·L -1 +NAA0.5mg·L -1 + sucrose 30.0g·L -1+AC 0.05g·L -1 + Carrageenan 7.0g·L -1 (pH=5.8±0.2), add 0, 2, 4 and 6mg·L to this medium respectively -1 Copper sulfate was us...

Embodiment 2

[0055] Addition of copper sulfate in the medium of embodiment 2 promotes proliferation and differentiation of "Dharma Molan" rhizomes

[0056] In order to find ways to improve the rhizome proliferation and differentiation efficiency of "Dharma Molan", the effect of copper sulfate on the proliferation and differentiation of "Dharma Molan" rhizomes was studied. The specific method is as follows:

[0057] S1. Culture medium design and preparation include the following steps:

[0058] S11. Culture medium design: the basic proliferation medium is MS+6-BA 2.0mg·L -1 +NAA 0.5mg·L -1 +AC0.3g·L -1 + sucrose 30.0g·L -1 + Carrageenan 7.0g·L -1 (pH=5.8±0.2), add 0, 2, 4 and 6mg·L to this medium respectively -1 Copper sulfate was used for proliferation test. The basic differentiation medium is MS+6-BA3.0mg·L -1 +NAA0.5mg·L -1 + sucrose 30.0g·L -1 + Carrageenan 7.0g·L -1 (pH=5.8±0.2), add 0, 2, 4mg·L -1 Copper sulfate was used for differentiation test.

[0059] S12. Culture pre...

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Abstract

The invention relates to a method for improving the proliferation and differentiation efficiency of Chinese orchid rhizomes. The method comprises the steps of: inoculating the rhizomes on the culture medium and cultivating them; adding 2 to 6 mg·L ‑1 of copper sulfate. The research of the present invention found that adding appropriate concentration of copper sulfate to the conventional culture medium can significantly increase the proliferation rate and differentiation rate of the rhizome of the orchid, and significantly improve the efficiency of the industrial reproduction of the orchid. It is a new variety of "Yuxianglan" Promote and lay the technical foundation for the industrialization of Guolan. In addition, this method does not need to change the production procedure, hardly increases the cost, can significantly improve the industrial production efficiency of orchid seedlings, and has important practical significance for establishing an efficient industrial production technology system for orchids and promoting the industrialization of orchids.

Description

technical field [0001] The invention relates to the field of plant tissue culture in the field of biotechnology, in particular to a method for improving the proliferation and differentiation efficiency of Chinese orchid rhizomes. Background technique [0002] Chinese Cymbidiums refer to the ground species of orchids of the genus Cymbidium (including Chunlan, Cymbidium, Jianlan, Molan, Hanlan and Rabbit-eared Orchid, etc.) Specific collective hybrids such as Yutulan (Cymbidium Jade Hare) or varieties. Guolan has a long history of cultivation and profound cultural connotation in China, and the market prospect is broad. In 2015, the domestic annual sales of Guolan was 1.224 billion yuan, of which Molan accounted for the highest proportion. With the development of my country's economy, the continuous improvement of people's quality of life and the popularization and promotion of orchid culture, Guolan will become more and more popular among domestic consumers. At the same tim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾瑞珍徐礼谱李娟李晓红郭和蓉谢利张志胜
Owner SOUTH CHINA AGRI UNIV
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