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Method for regenerating sapium japonicum plant by inducing sapium japonicum stem rapidly

A technology of Chinese tallow tree and regenerated plants, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., to achieve the effect of promoting the planting industry

Inactive Publication Date: 2014-11-12
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the current low yield of Chinese tallow tree seeds, long germination cycle, and immature cutting and grafting propagation techniques, which are difficult to meet the needs of large-scale planting seedlings problem, providing a method for inducing regenerated plants directly through shoot segments

Method used

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  • Method for regenerating sapium japonicum plant by inducing sapium japonicum stem rapidly

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Experimental program
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Effect test

Embodiment 1

[0023] The present embodiment is to take branch not through hydroponics rejuvenation and explant adopts conventional disinfection method as contrast, comprises the following steps:

[0024] (1) Explant culture: In the middle and early February, when the sap begins to flow but the buds have not germinated, select a 2-4-year-old high-quality mother plant of Chinese tallow tree in the nursery, cut off healthy branches, and rinse them with running water After that, hydroponic rejuvenation was carried out in a culture room with a daytime culture temperature of (27±2)°C, a nighttime culture temperature of (23±2)°C, a light intensity of 2 000 Lx, and a light intensity of 12 h / d. The formula of hydroponic nutrient solution is: calcium nitrate tetrahydrate 150 mg / L, potassium nitrate 80 mg / L, ammonium nitrate 15 mg / L, potassium dihydrogen phosphate 20 mg / L, magnesium sulfate 80 mg / L. The nutrient solution was changed every 3 days, and the newly grown shoots were used as explants after ...

Embodiment 2

[0035] In this embodiment, direct light culture is used as a control without dark culture during the induction culture of stem segments, including the following steps:

[0036] (1) Explant culture: In the middle and early February, when the sap begins to flow but the buds have not germinated, select a 2-4-year-old high-quality mother plant of Chinese tallow tree in the nursery, cut off healthy branches, and rinse them with running water After that, hydroponic rejuvenation was carried out in a culture room with a daytime culture temperature of (27±2)°C, a nighttime culture temperature of (23±2)°C, a light intensity of 2 000 Lx, and a light intensity of 12 h / d. The formula of hydroponic nutrient solution is: calcium nitrate tetrahydrate 150 mg / L, potassium nitrate 80 mg / L, ammonium nitrate 15 mg / L, potassium dihydrogen phosphate 20 mg / L, magnesium sulfate 80 mg / L. The nutrient solution was changed every 3 days, and the newly grown shoots were used as explants after about 15 days...

Embodiment 3

[0046] In this embodiment, when the adventitious buds are induced, they are sealed with a closed parafilm, and 4.5% white sugar and 0.7% agar are added to the culture medium as a control, including the following steps: .

[0047] (1) Explant culture: In the middle and early February, when the sap begins to flow but the buds have not germinated, select a 2-4-year-old high-quality mother plant of Chinese tallow tree in the nursery, cut off healthy branches, and rinse them with running water After that, hydroponic rejuvenation was carried out in a culture room with a daytime culture temperature of (27±2)°C, a nighttime culture temperature of (23±2)°C, a light intensity of 2 000 Lx, and a light intensity of 12 h / d. The formula of hydroponic nutrient solution is: calcium nitrate tetrahydrate 150 mg / L, potassium nitrate 80 mg / L, ammonium nitrate 15 mg / L, potassium dihydrogen phosphate 20 mg / L, magnesium sulfate 80 mg / L. The nutrient solution was changed every 3 days, and the newly g...

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Abstract

The invention discloses a method for regenerating a sapium japonicum plant by inducing a sapium japonicum stem rapidly. The method for regenerating the sapium japonicum plant by inducing the sapium japonicum stem rapidly comprises the steps of explant culture, explant treatment, adventitious bud induction, adventitious bud subculture multiplication, adventitious bud rooting, bed arranging and matrix preparing, and domestication and transplantation. According to the method for rapidly regenerating the sapium japonicum plant by inducing the sapium japonicum stem, water planting and seedling regenerating are conducted to a sapium japonicum branch through a self-prepared nutrient solution, a new twig can be obtained to serve as an explant, the contamination rate of the explant can be controlled to be lower than 5% according to the magnetic stirring type sterilization method, and the contamination rate of the explant is lowered by 15% compared with a conventional treatment method. According to the method for regenerating the sapium japonicum plant by inducing the sapium japonicum stem rapidly, the culture cycle is short, the seedlings are germinated rapidly and are uniform, the culture cost is low, the culture process is simple, the contamination rate of sapium japonicum explants is lower than 5%, the adventitious bud induction rate reaches above 85%, the multiplication coefficient reaches 8.0, the vitrification rate is lowered by 5.5%, the adventitious bud rooting rate reaches 95%, and the survival rate of transplanting reaches above 95%, the requirement for large-scale culture of sapium japonicum can be effectively met, and planting of the sapium japonicum is promoted.

Description

[0001] technical field [0002] The invention relates to a method for quickly inducing and regenerating plants from the stem section of Chinese tallow tree, and belongs to the technical field of tissue culture for rapid asexual propagation of seedlings. Background technique [0003] Whitewood Sapium[ Sapium japonicum (Sieb. et Zucc.) Pax et Hoffm.] is a closely related plant of the genus Sapium genus Sapium in Euphorbiaceae. Its seeds are larger than Sapium sativa and do not have white waxy arils. It is a wild oily plant. The plant height is 1-8 m, the flowering period is from May to June, the seeds are oblate, mature in November, and the diameter is 6-9 mm. After planting for 2-3 years, it can produce 5-10 kg of seeds, and enters the peak production period in 5 years, with an average of more than 50 kg of seeds and a maximum yield of 300 kg. It is a rare and important energy tree species with good development value, but limited to Insufficient grasp of the cultivatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 高柱王小玲刘腾云余发新幸学俊
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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