The invention discloses a cyclocarya paliurus adventitious bud inducing and subculture multiplication culture method, and belongs to the technical field of plant tissue culture. Young cyclocarya paliurus twigs serve as explants, and before being subjected to inducing and multiplication culture, the explants are sterilized; during sterilization, alcohol is adopted to treat the explants for 20-30 s,and sodium hypochlorite is adopted to treat the explants for 3-4 min; a culture medium for cyclocarya paliurus adventitious bud inducing is prepared from WPM, 6-BA, saccharose and agar powder, and pHis adjusted to be 5.75-5.85; a culture medium for cyclocarya paliurus adventitious bud multiplication culture is prepared from WPM, IBA, saccharose and agar powder. According to the cyclocarya paliurus adventitious bud inducing and subculture multiplication culture method, through material optimization, sterilization and the culture formulas, a large quantity of adventitious bud materials which are healthy and strong, have spread leaves and grow rapidly, are obtained within a short time, the foundation is laid for building an efficient cyclocarya paliurus in-vitro rapid propagation system, and the cyclocarya paliurus adventitious bud inducing and subculture multiplication culture method also has great significance of protecting, developing and utilizing cyclocarya paliurus germplasm resources.