30results about How to "High rate of adventitious bud induction" patented technology

The invention discloses a method for regeneration plant of tung oil tree leaf, and belongs to the technology field of rapid propagation of tung oil tree. The method comprises the steps of adventitious bud induction directly by tung oil tree leaf, subculture, multiplication culture, strong seedlings culture, rooting culture, acclimatization and transplantation, which not only provides a path for rapid breeding of excellent tung oil tree plants, and lays a solid foundation for improving resistance of the tung oil tree, tung oil tree output and properties of oil quality later on, and for building a tung oil tree genetic system.

The invention discloses a method for obtaining regeneration plants by use of camellia oleifera hypocotyl and belongs to the field of plant cell engineering. The method mainly comprises the step that adventive buds are induced by camellia oleifera hypocotyl. Through multiplicative subculture, strong seedling culture, rooting culture, acclimatization and transplant and the like of the adventive buds, not only is a new way provided for quick propagation of favorable camellia oleifera plants, but also a solid foundation is laid for improving the resistivity of camellia oleifera, accelerating the breeding process of camellia oleifera molecules and improving the oil quality and other properties by the gene engineering method, as well as building a camellia oleifera genetic system.

The invention belongs to the field of plant cell engineering and discloses a method for acquiring regeneration plants by adventitious bud inducement of camellia oleifera radicles. The method mainly includes steps of direct inducement of adventitious buds through camellia oleifera radicles , subculture multiplication, best seedling cultivation, rooting cultivation, acclimatization, transplantation and the like. Not only is an approach for rapid propagation of excellent camellia oleifera varieties provided but also a technical support is provided for shortening camellia oleifera breeding process and improving resistibility of camellia oleifera by means of gene engineering.

The invention discloses an establishing method for an efficient regeneration system for multiple-genotype cowpeas. The method comprises the following steps: pretreatment, induction of adventitious buds, subculturing, adventitious bud elongating, rooting culture, seedling hardening and transplanting. In the pretreatment process, 6-BA with the concentration of 1-4 mg / L or TDZ with the concentration of 0.001-0.1 mg / L is used as a plant growth regulator; the induction process of adventitious buds, a composition of 6-BA and KT or a composition of KT and IBA is used as a plant growth regulator, wherein the concentration of the 6-BA is 0.8-1.2 mg / L, the concentration of the KT is 0.02-2 mg / L, and the concentration of the IBA is 0.15-0.25 mg / L; in the adventitious bud elongating process, 6-BA and IBA are used as a plant growth regulator, wherein the concentration of the 6-BA is 0.5 mg / L, and the concentration of the IBA is 0.1-0.3 mg / L. The efficient regeneration system is not only suitable for regeneration culture of the multiple- genotype cowpeas, but also has the advantages of high induction rate, multiple induced adventitious buds, long adventitious buds, high dry weight of adventitious buds, easy implementation conditions and has a good promotion prospect.

The invention discloses a melon regeneration in vitro method. The melon regeneration in vitro method provided by the invention comprises the following steps: (1) taking a cotyledon of a melon as an explant, inoculating the explant into a shoot induction medium for culturing to obtain an adventitious bud tussock; (2) placing the adventitious bud tussock obtained in the step (1) to a shoot elongation medium A for culturing to obtain an adventitious bud; (3) placing the adventitious bud obtained in the step (2) into a rooting medium for culturing to obtain a melon regeneration seedling. The melon regeneration in vitro method provided by the invention has the advantages of high adventitious bud inductivity, low vitrification degree, short regeneration period and the like, and by applying the method in the melon genetic transformation, a relatively high transformation rate can be obtained.

The invention discloses an oil sunflower inbred line in vitro culture method. The method includes the following steps: (1) performing sterile seedling obtaining, taking oil sunflower inbred line seedsto disinfect after wrapping the seeds with gauze, and inoculating the disinfected seeds to a solid medium to cultivate to obtain sterile seedlings; (2) performing adventitious bud induction, cuttingcotyledonary nodes of the inoculated 5-day-old sterile seedlings, cutting 1-2 small cuts vertical to leaf veins, inoculating the cotyledonary nodes on an adventitious bud induction culture medium to induce adventitious buds; (3) performing the root growing of the adventitious buds, cutting the buds from explants to transfer to a root-growing culture medium to induce roots to grow when the inducedadventitious buds are grown to 1-2 cm; and (4) performing seedling hardening and transplanting when the roots are grown to 3-5 cm. The method takes oil sunflower inbred lines as a test material; technical methods of obtaining regeneration plants through oil sunflower in vitro culture can be optimized; and foundations of realizing genetic transformation of oil sunflowers can be established.

The invention discloses an efficient euonymus fortunei regeneration culture medium group. The efficient euonymus fortunei regeneration culture medium group is composed of a callus subculture medium, an inducing bud growth culture medium, a regenerated seedling subculture medium and a regenerated seedling rooting culture medium, wherein the callus subculture medium contains 0.5mg / L of NAA (Naphthylacetic acid) and 1mg / L to 1.5mg / L of BA (Butyl Acrylate); the inducing bud growth culture medium contains 0.1mg / L of the NAA and 0.5mg / L of the BA; the regenerated seedling subculture medium contains 0.05mg / L of the NAA and 1mg / L of the BA; the regenerated seedling rooting culture medium contains 0.6mg / L of the NAA. By adopting the callus subculture medium in the culture medium group disclosed by the invention, euonymus fortunei calluses can grow relatively rapid and the calluses with good differentiation and inducing capabilities are cultured; the inducing rate of the inducing bud growth culture medium on euonymus fortunei adventitious buds reaches 16.7 percent; the regenerated seedling subculture medium has a remarkable promotion effect on the growth of euonymus fortunei regenerated seedlings and the regenerated seedling rooting culture medium has a good rooting effect on euonymus fortunei.

The invention discloses a tissue culture method of dracocephalum rupestre hance and application thereof. The tissue culture method of the dracocephalum rupestre hance comprises the following steps: (1) culturing dracocephalum rupestre hance seeds after disinfection in sterile condition so as to obtain sterile seedlings; 2) culturing terminal buds of the sterile seedlings as explants in an inducingmedium so as to obtain cluster buds; and 3) culturing single buds taken from the cluster buds in a rooting medium so as to obtain regenerated dracocephalum rupestre hance seedlings. The tissue culture method of the dracocephalum rupestre hance adopts the terminal buds of the sterile seedlings as the explants; and the explants are cultured in the inducting medium containing 6-benzylaminoadenine (6-BA) or thidiazuron (TDZ) so as to carry out adventitious bud culture. The tissue culture method of the dracocephalum rupestre hance disclosed by the invention is simple, fast, improved in adventitious bud induction rate, and capable of ensuring quick breeding so as to obtain regenerated dracocephalum rupestre hance plants.

The invention discloses a method for inducing and culturing hybrid sweetgum tetraploid. The method comprises the following steps: correspondingly performing bud primordium predifferentiation culture on leaves and petioles of separated sterile plants of hybrid sweetgum; and then performing colchicine inducing culture on the leaves and petioles subjected to the predifferentiation culture. With the adoption of the method, the hybrid sweetgum tetraploid inducing rate is high, and the yield of the hybrid sweetgum tetraploid is high; in addition, the artificial hybrid sweetgum allotetraploid is induced and obtained for the first time; the quantity of two allogenetic chromosomes is copied; the hybridization effect and the ploidy effect are combined, so that heteromorphosis can be realized greatly, and as a result, the probability of further improving sweetgum genetic gain and selective breeding is increased.

The invention aims to provide a Valeriana Fauriei Briq. tissue culture breeding method. The method is as below: 1, using young stems of Valeriana Fauriei as explants and conducting aseptic treatment; 2, placing sterile Valeriana Fauriei stems on a solid culture medium to induce adventitious buds; 3, inoculating the adventitious buds onto the solid medium for rooting and seedling, induced; and 4, transplanting the tissue culture seedlings to the culture medium for hardening seedling cultivation. The invention is not limited or influenced by natural conditions and, has the characteristics of fastness and easiness for large scale. The invention can ease the problem of shortage of wild Valeriana Fauriei resources and of destruction of ecological balance caused by overexploitation.

The invention discloses a method for tissue culture and rapid propagation of rubus coreanus. The method sequentially comprises the following steps: (1) disinfecting and inoculating a stem section of the rubus coreanus: taking a new stem, put forth in that very year, of the rubus coreanus, cutting into a moderately lignified stem section with an axillary bud, and inoculating; (2) inducing multiple shoots; (3) proliferating adventitious buds; (4) culturing roots of a regenerated plant; (5) acclimatizing and transplanting. Culture mediums with different components and proportions are adopted in the steps. With the adoption of the method provided by the invention, a rubus coreanus plant can be rapidly acquired to promote industrial production, the induction rate of the adventitious buds of the multiple shoots of the rubus coreanus is up to 92.5%, the propagation time of the adventitious buds is up to 7.2, and the transplanting survival rate of the plant is up to more than 95%.

The invention discloses a method for inducing and multiplying adventitious buds of Cyclocarya paliurus, belonging to the technical field of plant tissue culture. Select the young shoots of Cyclocarya paliurus as explants, before the explants are induced and value-added culture, the explants are first sterilized and disinfected; the sterilized treatment adopts alcohol treatment for 25-30s, sodium hypochlorite treatment for 3-4min; Adventitious bud induction medium formula: WPM+6‑BA+sucrose+agar powder, and adjust pH to 5.75‑5.85; Cyclocarya adventitious bud proliferation medium formula: WPM+IBA+sucrose+agar powder. The present invention obtains a large amount of adventitious bud materials with strong growth, stretched leaves and high growth in a relatively short period of time by optimizing materials, sterilizing treatment and cultivating formula, laying the foundation for establishing an efficient in vitro rapid propagation system of Cyclocarya paliurus, and also It is of great significance to the protection, development and utilization of Cyclocarya paliurus germplasm resources.

The invention belongs to the technical field of plant culture and particularly relates to a method for tissue culture and rapid propagation of polygonatum filipes. The method for tissue culture and rapid propagation of polygonatum filipes comprises the following steps that (1), polygonatum filipes flower buds are disinfected and inoculated; (2), cluster buds are induced; (3), adventitious buds are proliferated; (4), rooting culture of regenerated plants is performed; (5) acclimatization and transplanting are performed. By adopting the method and compared with reported literatures, the preprocessing time can be shortened, the explant pollution rate is low, the adventitious bud generating time is shortened, accordingly regenerated polygonatum filipes plants are obtained more quickly, and industrial production is promoted. By adopting the method, the adventitious bud inducing rate of the cluster buds of the polygonatum filipes is as high as 90.0%, the number of the adventitious buds is 8.4, the adventitious bud proliferation rate can be up to 7.7, and the survival rate of transplanted plants can be up to 95% or above.

The invention discloses a method for obtaining regeneration plants by use of camellia oleifera hypocotyl and belongs to the field of plant cell engineering. The method mainly comprises the step that adventive buds are induced by camellia oleifera hypocotyl. Through multiplicative subculture, strong seedling culture, rooting culture, acclimatization and transplant and the like of the adventive buds, not only is a new way provided for quick propagation of favorable camellia oleifera plants, but also a solid foundation is laid for improving the resistivity of camellia oleifera, accelerating the breeding process of camellia oleifera molecules and improving the oil quality and other properties by the gene engineering method, as well as building a camellia oleifera genetic system.

The invention belongs to the field of plant cell engineering and discloses a method for acquiring regeneration plants by adventitious bud inducement of camellia oleifera radicles. The method mainly includes steps of direct inducement of adventitious buds through camellia oleifera radicles , subculture multiplication, best seedling cultivation, rooting cultivation, acclimatization, transplantation and the like. Not only is an approach for rapid propagation of excellent camellia oleifera varieties provided but also a technical support is provided for shortening camellia oleifera breeding process and improving resistibility of camellia oleifera by means of gene engineering.

The invention relates to a method for buildsing-up a regeneration system for beach mallow, using beach mallow mature embryos as explants, comprising following steps: (1)explants surface sterilization, (2)tissue culture, with MS basal culture medium containing 0.5-2.0mg / L of 2,4-dichlorophe noxyacetic acid,(3)tissue subculture, with MS basal culture medium containing 0.3mg / L of heteroauxin and 0.3mg / L of zeatin, (4)adventitious bud culture, with MS or 1 / 2 MS basal culture medium containing 0.1-0.2mg / L of heteroauxin and 0.3-0.5mg / L of zeatin, (5) rooting culture, with MS basal culture medium free of hormones, (6)obtusifolia seedlings transplanting and hardening. The method is provided with a tissue culture rate, a adventitious bud culture rate, a rooting rate up to 86.8%,64.2% and 95.2% respectively, and the survival rate of regenerating seedlings up to 80%.

The invention discloses a method for acquiring and propagating nervilia fordii aseptic rhizomes. The method is characterized in that disinfected explants are arranged in induction culture media, adventitious bud induction is carried out on the disinfected explants, the explants germinate to obtain the aseptic rhizomes, 1 / 2 MS is used as a basic culture medium for the induction culture media, agarwith the concentration of 5g L<-1>, sucrose with the concentration of 30 g L<-1>, 6-benzyladenin with the concentration of 1.5 mg L<-1> and naphthylacetic acid with the concentration of 0.3 mg L<-1> are added into the basic culture medium, the pH (potential of hydrogen) values of the induction culture media are 6.8, and the culture conditions include the temperatures of 25-27 DEG C, the illumination intensity of 2100 lux, the illumination time of 10-12 h / d and the culture time of 15-20 d. The method for acquiring and propagating the nervilia fordii aseptic rhizomes has the advantages of high adventitious bud induction rate and applicability to factory production.

The invention discloses a method for inducing adventitious buds by utilizing pinus koraiensis germination-accelerated seed germination buds, and relates to the field of forest seedling propagation. The invention aims to solve the problems that current organogenesis of pinus koraiensis is difficult and a differentiation rate of adventitious buds is low. The method comprises the following steps: (1)taking materials of explants and performing disinfection treatment; (2) performing induction differentiation cultivation on the adventitious buds; (3) performing elongation cultivation on the adventitious buds; and (4) performing rooting induction cultivation on regenerated plants. The method specially comprises the following steps: using germination-accelerated pinus koraiensis seeds as the explants, performing inoculation on a differentiation culture medium by the explants, and performing cultivation for 30 days; and after the adventitious buds are obtained, transferring the adventitious buds to an elongation culture medium, continuing cultivation for 60 days (performing subculture once in the process), when cluster seedlings grow to a length of 2-3 cm, performing cutting, performing separation, transferring the separated seedlings to a rooting medium, and performing domestication. According to the method disclosed by the invention, an induction rate of the adventitious buds reaches92.2%, average quantity of buds per explant is 10-12, and after elongation growth cultivation is performed for 60 days, a height of each bud is 3.5 cm, and the rooting rate reaches 52%.

The invention aims to provide a Valeriana Fauriei Briq. tissue culture breeding method. The method is as below: 1, using young stems of Valeriana Fauriei as explants and conducting aseptic treatment; 2, placing sterile Valeriana Fauriei stems on a solid culture medium to induce adventitious buds; 3, inoculating the adventitious buds onto the solid medium for rooting and seedling, induced; and 4, transplanting the tissue culture seedlings to the culture medium for hardening seedling cultivation. The invention is not limited or influenced by natural conditions and, has the characteristics of fastness and easiness for large scale. The invention can ease the problem of shortage of wild Valeriana Fauriei resources and of destruction of ecological balance caused by overexploitation.

The invention discloses a cyclocarya paliurus adventitious bud inducing and subculture multiplication culture method, and belongs to the technical field of plant tissue culture. Young cyclocarya paliurus twigs serve as explants, and before being subjected to inducing and multiplication culture, the explants are sterilized; during sterilization, alcohol is adopted to treat the explants for 20-30 s,and sodium hypochlorite is adopted to treat the explants for 3-4 min; a culture medium for cyclocarya paliurus adventitious bud inducing is prepared from WPM, 6-BA, saccharose and agar powder, and pHis adjusted to be 5.75-5.85; a culture medium for cyclocarya paliurus adventitious bud multiplication culture is prepared from WPM, IBA, saccharose and agar powder. According to the cyclocarya paliurus adventitious bud inducing and subculture multiplication culture method, through material optimization, sterilization and the culture formulas, a large quantity of adventitious bud materials which are healthy and strong, have spread leaves and grow rapidly, are obtained within a short time, the foundation is laid for building an efficient cyclocarya paliurus in-vitro rapid propagation system, and the cyclocarya paliurus adventitious bud inducing and subculture multiplication culture method also has great significance of protecting, developing and utilizing cyclocarya paliurus germplasm resources.

The invention provides a method for improving the seedling rate of tissue culture seedlings of hylocereus undulatus britt. The method comprises the following steps: carrying out adventitious bud induction, synchronous multiplication and rooting seedling culture, wherein hylocereus undulatus britt barbed seats and lignification shoots with small barbs and fluff on the barbed seats are used as explants; when lateral branches of adventitious buds grow, base roots of adventitious bud base parts grow and aerial roots of the lateral branches grow, carrying out hardening seedling; after the hardening seedling, taking out seedlings from a bottle; cutting off the lateral branches with the aerial roots and main stems with the base roots and planting in a culture medium; after the lateral branches and the main stems are survived, growing new roots; and putting the lateral branches and the main stems into pots. According to the method, the production period of the tissue culture seedlings of the hylocereus undulatus britt is short, the adventitious bud induction rate is high, the multiplication and the rooting are synchronous, the multiplication coefficient is more than 5.45 and the rooting rate is 100%; the growth vigor of multiplied seedlings is strong and the transplanting survival rate is more than 95%; one inoculated adventitious bud can grow 3-4 lateral branches with the aerial roots, namely one inoculated adventitious bud can grow 4-5 grown-up seedlings with the roots; the seedling rate of the tissue culture seedlings of the hylocereus undulatus britt is increased by 3-4 times.

The invention discloses a method for rapidly breeding paris polyphylla, mainly aiming at solving the problem of the existing paris polyphylla tuber breeding way in the prior art that, only one bud can be bred by using one tuber, a smaller root is formed, and growing is slow, so that the demand of the existing market on polyphylla breeding cannot be met. The perennial and robust paris polyphylla rhizomes which are not damaged and do not have plant diseases and insect pests are selected as a provenance for breeding; the method comprises the steps of digging out the fresh paris polyphylla rhizomes, washing and then drying in the air, and cutting off adventitious roots on the rhizomes; after that, washing stem internodes with sterile water, then cutting off buds on the stem internodes, and cutting the stem internodes of the rhizomes into blocky structures; inoculating the blocky structures into a breeding culture medium for culturing; when a first true leaf and five adventitious roots grow on one blocky structure, transplanting the blocky structure into soft soil for growing. After the scheme is adopted, the aims of rapidly breeding the paris polyphylla and enabling the paris polyphylla to take more roots are achieved.

The invention belongs to the technical field of plant cultivation, and in particular relates to a method for tissue culture and rapid propagation of Polygonatum long-stalked. A method for tissue culture and rapid propagation of Polygonatum long-stemmed Polygonatum, the method comprising the following steps: (1) disinfection and inoculation of flower buds of Polygonatum long-stemmed; (2) induction of clustered buds; (3) proliferation of adventitious buds; (4) Rooting culture of regenerated plants; (5) Hardening and transplanting. By adopting the method of the present invention, compared with the reported literature, the pretreatment time can be shortened, the explant contamination rate is low, and the adventitious bud generation time can be shortened, thereby obtaining long-stalked Polygonatum regenerated plants faster, which is beneficial to industrial production. By adopting the method of the present invention, the induction rate of adventitious buds of long-stemmed Polygonatum polygonatum clustered buds is as high as 90.0%, the number of adventitious buds is 8.4, the multiplying multiple of adventitious buds can reach 7.7, and the survival rate of transplanted plants can reach more than 95%.

The invention discloses a method for inducing and cultivating hybrid liquidambar tetraploids, which comprises the steps of carrying out pre-differentiation culture treatment on the leaves and petioles of the isolated aseptic plants of the hybrid liquid sweetgum respectively, and then treating the pre-differentiated cultured The leaves and petioles were induced by colchicine respectively. The method of the present invention has a high induction rate of the hybrid liquidambar tetraploid, and a high rate of obtaining the hybrid liquidambar tetraploid, and adopts the method of the present invention to induce and obtain the hybrid liquidambar artificial allotetraploid for the first time, and the number of two heterologous chromosomes Being copied, it has both hybridization effect and ploidy effect, and can produce variation to a greater extent, which provides the possibility to further improve the genetic gain of liquidambar and the selection of improved varieties.

The invention discloses a method for tissue culture and rapid propagation of A. japonica, which comprises the following steps in turn: (1) Disinfection and inoculation of the stem section of A. japonica; (2) induction of clustered buds; (3) proliferation of adventitious buds; (4) rooting culture of regenerated plants; (5) transplanting of hardened seedlings. In the above steps, culture media with different components and ratios are used. By adopting the method of the invention, it is possible to quickly obtain the bubble plant in the field, which is beneficial to industrialized production. By adopting the method of the present invention, the induction rate of adventitious buds of clustered buds of the transplanted bubble can reach as high as 92.5%, the multiplying multiple of adventitious buds can reach 7.2, and the transplanting survival rate of plants can reach more than 95%.