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Method for obtaining regeneration plants by use of camellia oleifera hypocotyl

A technology for regenerating plants and hypocotyls, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as failure to apply, no explants found for Camellia oleifera transgene, and inability to transgene plants

Active Publication Date: 2015-09-30
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many studies on the tissue culture of Camellia oleifera. Zhang Zhijun et al. used the cotyledons of Camellia oleifera clones to obtain regenerated plants through somatic embryogenesis. Li Ze et al. It is of great significance in cultivating rapid propagation, and cannot be applied to the transformation of Camellia oleifera genetic system to obtain transgenic plants
The most commonly used method for the transformation of plant genetic systems is the Agrobacterium-mediated method to infect plant leaves, hypocotyls, petioles, radicles and other organs, while the induction rate of adventitious buds induced by tea leaves is only 17.86%, and it cannot be applied to camellia oleifera at present. In terms of genetic system transformation, the Camellia oleifera transgene has not yet found a suitable explant

Method used

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  • Method for obtaining regeneration plants by use of camellia oleifera hypocotyl
  • Method for obtaining regeneration plants by use of camellia oleifera hypocotyl
  • Method for obtaining regeneration plants by use of camellia oleifera hypocotyl

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Experimental program
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Embodiment 1

[0037] Do the following in sequence:

[0038] 1. Select mature seeds of Camellia oleifera that year, remove the seed coats, rinse the seeds with tap water for 3-5 minutes, then soak them for 10-15 hours, change the water 2-3 times in the middle, and then use 75% of them in an ultra-clean workbench. Soak in alcohol for 20-30s, rinse with sterile water for 3-5 times, then use 0.1% HgCl 2 Sterilize for 3-5 minutes, rinse with sterile water 5-6 times, cut off most of the endosperm, inoculate the embryos with endosperm in WPM basic medium, cultivate in the dark for 2-3 days, then transfer to light for 20- Sterile seedlings can be obtained in 25 days; the culture temperature is 26±1℃, the light intensity is 2100-2200lx, and the light time is 12-14h / d.

[0039] Table 1 The effect of different disinfection treatment methods on the disinfection effect of Camellia oleifera seed embryos

[0040]

[0041] 2. Select the hypocotyl of the aseptic seedling of Camellia oleifera, cut the shaft sectio...

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Abstract

The invention discloses a method for obtaining regeneration plants by use of camellia oleifera hypocotyl and belongs to the field of plant cell engineering. The method mainly comprises the step that adventive buds are induced by camellia oleifera hypocotyl. Through multiplicative subculture, strong seedling culture, rooting culture, acclimatization and transplant and the like of the adventive buds, not only is a new way provided for quick propagation of favorable camellia oleifera plants, but also a solid foundation is laid for improving the resistivity of camellia oleifera, accelerating the breeding process of camellia oleifera molecules and improving the oil quality and other properties by the gene engineering method, as well as building a camellia oleifera genetic system.

Description

Technical field [0001] The invention belongs to the field of camellia oleifera cell engineering, and specifically relates to a tissue culture method for inducing adventitious buds by using oleifera hypocotyls as explants, and then obtaining complete test tube seedlings. Background technique [0002] Camellia Oleifera (Camellia Oleifera) is an evergreen shrub or small tree in the Camellia genus (Theaceae). It is a unique woody edible oil tree species in my country. It is called the four largest woody oil plants in the world together with olive, oil palm and coconut. The content of unsaturated fatty acids in tea oil is more than 90%. It has good color and fragrant flavor, unique flavor, rich nutrition, durable storage, and has the functions of lowering blood pressure, blood lipids and softening blood vessels. It plays an important role in the prevention of cardiovascular diseases. It is a high-quality edible oil One is known as the "Oriental Olive Oil". At the same time, the by-pr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张琳李泽谭晓风林青邹锋曾艳玲龙洪旭赵广
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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