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Building method of eucapyptus urophylla leaf-induced plantlet regeneration system

A technology for establishing methods and leaves, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve problems such as low regeneration rate, and achieve high rooting rate, high induction rate, and good seedling effect.

Inactive Publication Date: 2016-08-03
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is still found that there is a problem of low regeneration rate

Method used

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  • Building method of eucapyptus urophylla leaf-induced plantlet regeneration system
  • Building method of eucapyptus urophylla leaf-induced plantlet regeneration system
  • Building method of eucapyptus urophylla leaf-induced plantlet regeneration system

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Embodiment Construction

[0012] The present invention is further explained below in conjunction with the examples, but the examples do not limit the present invention in any form.

[0013] The test material is the leaves of sterile rooted seedlings cultured for 20-30 days on the rooting medium. The rooting medium adopts improved MS medium, that is, 1 / 2MS medium, 7g / L agar and 30g / L sucrose. Proliferation medium adopts improved B5 medium, that is, add 0.20 mg.L to B5 medium -1 The 6-BA, leaf regeneration induction basic medium is based on the improved MS medium; add 7.0g.L -1 of agar and 30.0g.L -1 of sucrose. The specific culture conditions are as follows: the light time is 16h / 8h, the light intensity is 1500-2000lx, and the culture temperature is about 25±2°C.

[0014] Add 0.0025, 0.005, 0.0075, 0.01, 0.025, 0.05mg.L to the regeneration induction basic medium -1 TDZ (Thidiazuran), each treatment had 40-60 leaf explants, repeated 3 times, and analyzed the effect of adding different concentrations...

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Abstract

The invention provides a building method of a eucapyptus urophylla leaf-induced plantlet regeneration system. The method comprises the following steps: selecting vigorously growing buds which are 1.5-2.0cm in a proliferation culture medium and putting the buds into a rooting medium for rooting cultivation for 20-30 days; putting the taken leaves of sterile rooting seedlings into a regenerated induced medium for 30-40 days, transferring the induced adventitious buds into an adventitious bud culture medium for buds elongation cultivation once after 15-20 days and transferring 1.5-2.0cm adventitious buds into the rooting medium for rooting induction. A stable and efficient eucapyptus urophylla leaf regeneration system is built by optimizing the conditions of hormone, major elements, explant state and the like; a technical foundation is established for researches on cell breeding, genetic engineering and the like; the method is high in induction percentage of adventitious shoots, good in seeding strengthening effect and high in rooting rate.

Description

technical field [0001] The invention relates to the field of eucalyptus cultivation, in particular to a method for establishing a plant regeneration system induced by eucalyptus eucalyptus leaves. Background technique [0002] Eucalyptus (Eucalyptus), as the largest afforestation tree species in South China, has 4.4 million hectares of eucalyptus plantations (Xie Yaojian, 2011). Natural forests and ecological forests have been established, making great contributions to the construction of ecological civilization and timber safety in my country. With the continuous expansion of the cultivation area, there are problems that cannot be solved by conventional breeding such as insect pests such as inchworms, bacterial wilt and other diseases, freezing damage caused by the northward shift of cultivation, high costs of weeding and fertilization, and specific material requirements. Transferring functional genes with specific traits into forest trees through transgenic technology to o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 范春节曾炳山裘珍飞
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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