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Juvenile form treating method of mature citrus explant material

A treatment method and explant technology, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of pollution, seriousness, and slow progress in stem segment genetic transformation research, and achieve low pollution rate and regeneration efficiency high effect

Inactive Publication Date: 2013-08-28
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, with the emergence of mature characteristics of citrus adult materials, it is difficult to differentiate explant cells in in vitro culture, and the pollution is serious, which leads to slow progress in the genetic transformation of citrus adult internode stem segments, which seriously limits the practical application of citrus transgenics.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1 Bingtang orange adult state material example

[0011] The present embodiment test material is rock sugar orange, specifically comprises the following steps:

[0012] (1) First, use the full buds on the mother tree grafted for more than 5 years as the scion, and use the potted sour orange with an annual diameter of more than 0.6 cm as the rootstock, use the "bud grafting method" for grafting, and the grafting interface is the surface above the soil (at the root neck) At 12 cm upwards, after the grafted seedlings have grown for one year, take the full buds as the scion, and the potted sour orange with an annual diameter of more than 0.6 cm as the rootstock, and use the "bud grafting method" for grafting again, and the grafting interface is the surface above the soil (root neck 12 cm upward, after grafting, bend the rootstock 10 cm upward from the grafting interface along the side of the grafting surface for bending anvil treatment. Cut off the part of the ro...

Embodiment 2

[0016] Example 2 Transformation example of Egyptian sugar orange adult state internode stem segment

[0017] The test material is Egyptian sugar orange, and the juvenile treatment method is the same as in Example 1. By this method, 200 internode stem segments are used. In this example, the adventitious bud regeneration rate is as high as 72.4%, and the pollution rate drops to 0.

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PUM

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Abstract

The invention discloses a juvenile form treating method of a mature citrus explant material. The juvenile form treating method comprises the following steps of: (1) repeatedly cross-grafting mature citrus scions on infantile stocks; (2) obtaining the explant material; and (3) regenerating adventitious buds. The juvenile form treating method through which the mature citrus scions are repeatedly cross-grafted on the infantile stocks is applicable to citrus varieties; the contamination rate of the treated mature citrus material is low, or even is 0; and the regeneration rate of the adventitious buds is high, and the induction rate of the adventitious buds is as high as 67.7%, so that the juvenile form treating method is particularly applicable to juvenile form of the mature citrus explant material of citrus varieties such as candy orange and sugar orange.

Description

technical field [0001] The invention relates to a juvenile treatment method of an adult explant material, in particular to a juvenile treatment method of an adult citrus explant material. Background technique [0002] Citrus is the largest fruit in the world. The world's citrus planting area reaches 8.72 million hectares, and the annual output of citrus exceeds 123.7 million tons. my country's citrus area has exceeded 2.21 million hectares, with an output of nearly 28.9 million tons, ranking first in the world. In citrus breeding research, using transgenic technology to improve citrus varieties, compared with conventional breeding methods, has the advantages of wide source of target genes, transformation system is not limited by genotype, and can directional change target traits. But at present, in the genetic transformation recipient system of citrus, the explants are mostly juvenile materials, which prolongs the transgenic breeding cycle. In order to overcome the problem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G1/06A01H4/00
Inventor 邓子牛杨莉胡威谢玉明葛红娟
Owner HUNAN AGRICULTURAL UNIV
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