Method for pear blade inducing adventitious buds to regenerate plant

A leaf and bud induction technology, applied in the field of plant cell engineering, can solve the problems of limited transformed varieties and narrow research scope

Inactive Publication Date: 2012-09-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the scope of pear leaf culture and transgenic research is narrow (mainly focused on Western pear), and the transformed varieties are limited. Therefore, on the basis of Western pear research, research on Oriental pear should be strengthened.

Method used

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  • Method for pear blade inducing adventitious buds to regenerate plant
  • Method for pear blade inducing adventitious buds to regenerate plant
  • Method for pear blade inducing adventitious buds to regenerate plant

Examples

Experimental program
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Effect test

Embodiment 1

[0020] (1) Select the healthy and young leaves of the test-tube plantlets, and cut 2 to 4 wounds perpendicular to the midrib of the leaves with a sterile blade on the ultra-clean workbench. On the culture medium, cultivate in the dark for 25 days and then transfer to the light. After 5 days, the edge curls to the back of the leaf and becomes cylindrical, and the leaves gradually turn yellow-green. After 25 days, a small amount of white and loose callus is formed on the veins and the incisions around them. tissue; after being placed under light for cultivation, the callus gradually turns light green, and adventitious buds are produced after inoculation for 30-35 days ( figure 1 ), the statistical adventitious bud differentiation rate was 66.67%;

[0021] The induction medium is: NN69+TDZ 2.0mg / L+IBA 0.3mg / L+sucrose 40g / L+agar 6g / L, pH value is 5.8; culture temperature is 25±2℃.

[0022] (2) After the leaves grow adventitious buds, transfer the adventitious buds to the prolifer...

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Abstract

The invention belongs to the field of plant cell engineering, and relates to a method for pear blade inducing adventitious buds to regenerate plant. The young blade of a test-tube plantlet is used as an explant and is placed on an ultra-clean work bench, 2-4 wounds are crosscut perpendicular to the blade midrib with a sterile blade, then the blade is inoculated on an adventitious bud induction medium, after being dark cultured for about 3 weeks, the blade is placed under light for culturing, and then is transferred to an enrichment medium after growing adventitious buds so as to promote the growth of buds; and after the adventitious buds grow to about 2cm, the blade is transferred to a rooting medium for culturing to obtain complete plants. The method is simple in operation, low in cost and good in result, and with the adoption of the method, a stable and efficient blade renewable system can be quickly established, the technical problems of pear blade adventitious bud regeneration induction can be effectively solved, so that the precondition can be provided for improving fruit tree variety through the two ways of genetic transformation of disease resistant gene and genome doubled polyploid cultivating.

Description

technical field [0001] The invention belongs to the field of plant cell engineering and relates to a method for inducing adventitious buds to regenerate plants from pear leaves. Background technique [0002] Pear is a plant of the genus Pyrus of the subfamily Maloldeae of the Rosaceae (Rosaeeae) family. It is an arboreal deciduous fruit tree with more than 20 species. Pears distributed in countries all over the world can be classified into two types: Oriental pears and Western pears. Oriental pear originated in my country, also known as Chinese pear; Western pear originates in Europe and Asia, and is the main cultivated variety in European and American countries. Pear fruit is not only beautiful in shape, soft or crisp in texture, rich in juice, sweet and refreshing, but also has high nutritional value. In addition to being eaten fresh, pear fruit can also be used to make preserved pears, pear paste, dried pears, wine making, and vinegar making etc., but also has high medi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张绍铃薛亚男吴俊陶书田齐开杰
Owner NANJING AGRICULTURAL UNIVERSITY
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