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Method for regeneration plant of tung oil tree leaf

A technique for regenerating plants and tung tree, which is applied in botany equipment and methods, plant regeneration, gardening methods, etc.

Active Publication Date: 2014-08-20
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The plant regeneration of woody plants through leaf cultivation has always been a difficult problem for forestry researchers in tissue culture. The method of tung tree regeneration by directly inducing adventitious buds through leaves has not been reported so far.

Method used

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  • Method for regeneration plant of tung oil tree leaf
  • Method for regeneration plant of tung oil tree leaf
  • Method for regeneration plant of tung oil tree leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Do the following in order:

[0044] 1. Select the mature seeds of the tung tree that year, take the seed coat, wash the kernels with tap water for 3-5 minutes, then soak for 20-30 hours, change the water 2-3 times in the middle, and then use 0.1% HgCl 2 Disinfect for 3-5 minutes, wash 4-6 times with sterile water, inoculate in 1 / 2MS basic medium, culture in dark for 2-3 days, and then transfer to light for 15-20 days to grow seedlings. The seedling rate can reach 91%. Sucrose 30g / L, agar 7g / L, pH 5.4-5.8; culture temperature 26±1°C, light intensity 1800-2000lx, light 14-16h / d;

[0045] Table 1 Effect of different disinfection treatment methods on the disinfection effect of tung seed embryos

[0046] 75% alcohol 0.1% mercury chloride pollution rate browning rate 0 2 27.69 0 0 3 3.22 0 0 4 3.13 0 0 5 2.98 1.23 10 3 3.01 4.98 10 5 0 46 20 5 0 56 30 5 0 65

[0047] 2. Select the leaves of tung tre...

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Abstract

The invention discloses a method for regeneration plant of tung oil tree leaf, and belongs to the technology field of rapid propagation of tung oil tree. The method comprises the steps of adventitious bud induction directly by tung oil tree leaf, subculture, multiplication culture, strong seedlings culture, rooting culture, acclimatization and transplantation, which not only provides a path for rapid breeding of excellent tung oil tree plants, and lays a solid foundation for improving resistance of the tung oil tree, tung oil tree output and properties of oil quality later on, and for building a tung oil tree genetic system.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of tung tree, in particular to a method for regenerating plants from leaves of tung tree. Background technique [0002] Vernicia fordii is a deciduous tree belonging to the genus Vernicia in the family Euphorbiaceae. It is a unique economic tree species in my country. The oil content of tung oil seeds is 50%-70%, about 94% of which are unsaturated fatty acids. The tung oil extracted from tung oil is a high-quality dry oil that can replace traditional diesel oil. With the decrease of crude oil and the aggravation of environmental protection pressure Today, tung oil has become an excellent bio-energy tree species. Tung oil is an excellent coating that resists cold and heat, moisture, rust, and corrosion. It is also the basic raw material for the synthesis of new composite materials and has a wide range of uses. In recent years, with the lack of energy and the improvement of tung oil proce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 谭晓风李泽张琳袁军龙洪旭曾艳玲周俊琴
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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