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A kind of method for rapid multiplication of Cyclocarya paliurus tissue culture seedlings

A fast technology for tissue culture seedlings, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of poor growth of seedlings, accelerate the industrial seedling cultivation of Cyclocarya paliurus, and low multiplication coefficient

Active Publication Date: 2020-04-07
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The purpose of the present invention is to solve the problem of low proliferation coefficient and poor seedling growth potential of Cyclocarya paliurus test-tube seedlings at present, and on this basis, provide a method for rapid multiplication of Cyclocarya paliurus tissue culture seedlings. The germinated sterile seedlings have a high multiplication coefficient, the seedlings grow vigorously, and the seedling effect is good. The key lies in the possibility of accelerating the industrial seedling cultivation of Cyclocarya paliurus

Method used

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  • A kind of method for rapid multiplication of Cyclocarya paliurus tissue culture seedlings
  • A kind of method for rapid multiplication of Cyclocarya paliurus tissue culture seedlings
  • A kind of method for rapid multiplication of Cyclocarya paliurus tissue culture seedlings

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Embodiment 1

[0037] Do the following in order:

[0038] 1. Select the seeds of the current year papaya papaya, air-dry and remove the wings for later use. Soak the seeds in concentrated sulfuric acid for 3 hours, remove the carbonized layer, rinse with tap water for 30 minutes, then soak for 3-5 days, change the water 1-2 times a day, and then soak them in 75% alcohol for 1-2 minutes in the ultra-clean workbench. Rinse with bacterial water 3-5 times, then use 0.1% HgCl 2 Disinfect for 30 minutes, rinse with sterile water 5-6 times, strip off the seed embryos under sterile conditions, inoculate in 1 / 2MS+3.0-5.0mg·L -1 GA 3 In the basic medium, after 2-3 days of dark culture, transfer to light for 20-25 days to obtain sterile seedlings, and the germination rate of seed embryos is above 90%; the culture temperature is 26±1°C, and the light intensity is 2100- 2200lx, light time 12-14h / d.

[0039] 2. Cut off the buds of the germ-germinated aseptic seedlings and inoculate them into A shown i...

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Abstract

The invention discloses a cyclocarya paliurus tissue culture seedling fast breeding method, and belongs to the technical field of plant tissue culture. Aseptic seedlings are mainly obtained through cyclocarya paliurus embryos; then, through the processes of subculture breeding culture, seedling strengthening culture, rooting culture and the like of aseptic seedlings, a great number of high-qualitytissue culture seedlings can be obtained. The method has the advantages that the tissue culture seedling growth coefficient is high; the proliferation bud growth condition is strong; a novel path isprovided for the fast breeding of cyclocarya paliurus test tube seedlings; the foundation is laid for the future cyclocarya paliurus factory seedling culture and cyclocarya paliurus resistance improvement by a gene engineering method and for the cyclocarya paliurus genetic system building.

Description

technical field [0001] The invention belongs to the field of tissue culture, and in particular relates to a tissue culture method for obtaining aseptic seedlings by using Cyclocarya paliurus seed embryos as explants, and then carrying out a large number of rapid multiplication cultures on the aseptic seedlings to obtain complete tissue culture seedlings. Background technique [0002] Cyclocarya is a deciduous tree of the genus Cyclocarya in the family Juglandaceae, also known as money tree, hemp willow, and green plum. Mountainous areas in Fujian, Sichuan and other provinces. Cyclocarya paliurus has great medicinal development value, and its leaves have obvious effects of lowering blood sugar, lowering blood pressure, clearing heat and detoxification, relieving pain, and improving human immunity. Cyclocarya paliurus mainly reproduces by sowing, but due to the low seed filling rate, relatively hard seed coat, and deep dormancy, the emergence rate is extremely low, which is n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李泽谭晓风吴玲利李建安曾艳玲韩航
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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