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Establishing method for efficient regeneration system for multiple-genotype cowpeas

A high-efficiency regeneration, multi-genotype technology, applied in the biological field, achieves the effects of high dry weight of adventitious buds, great promotion prospects, and simple implementation steps

Active Publication Date: 2015-03-04
SICHUAN AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to increase the number of main buds and improve the dry weight of adventitious buds is still rarely reported

Method used

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  • Establishing method for efficient regeneration system for multiple-genotype cowpeas
  • Establishing method for efficient regeneration system for multiple-genotype cowpeas
  • Establishing method for efficient regeneration system for multiple-genotype cowpeas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Select uniform and plump cowpea seeds, soak them in 75% alcohol for 1 minute, then soak them in 0.1% mercuric acid for 5 minutes, rinse them with sterile water for 5 times, and put them in a petri dish Inoculate the seeds on the culture medium with sterilized tweezers, and inoculate 10 seeds per bottle. Place cowpea seeds in MSBs containing 6-BA or TDZ 5 The culture medium was cultured for 5 days, the culture temperature was 25°C, the light intensity was 3000 lx, and the light time was 16 h / day. Then in the MSB containing 6-BA and KT or containing KT and IBA 5The culture medium was cultured for 7 days, the culture temperature was 23-27°C, the light intensity was 3000 lx, and the light time was 16 h / day. Six experimental groups were established. In the first experimental group, 6-BA and KT combination, the concentration of 6-BA is 0.8mg / L, the concentration of KT is 0.02mg / L, in the second experimental group, the combination of 6-BA and KT is used, the concentratio...

Embodiment 2

[0042] When the concentration of 6-BA in the current treatment is 3 mg / L, after the pretreatment, the cotyledon node with one cotyledon is cut from the aseptic seedling after the pretreatment, the node is 1 mm away from the epicotyl, and 5 mm away from the hypocotyl, Then inoculate on adventitious bud induction medium, inoculate 5-6 explants per bottle, and then put into MSB containing 6-BA and KT combination or KT and IBA combination 5 The culture medium was cultured for 7 days, the culture temperature was 23-27°C, the light intensity was 3000 lx, the light time was 16 h / day, the concentration of 6-BA was 0.8-1.2mg / L, and the concentration of KT was 0.02-2mg / L, the concentration of IBA is 0.15-0.25mg / L.

[0043] When the concentration of 6-BA in the current treatment was 3mg / L, the effects of the combination of 6-BA and KT and the combination of KT and IBA on the induction of adventitious buds are shown in Table 2.

[0044] Table 2

[0045]

[0046] It can be seen from T...

Embodiment 3

[0049] When the concentration of TDZ in the current treatment is 0.01mg / L, after the pretreatment, the cotyledon node with one cotyledon is cut from the aseptic seedling after the pretreatment, and the node is 0.5 mm away from the epicotyl, 5 mm away from the hypocotyl, and then Inoculate on Adventitious Bud Induction Medium, inoculate 5-6 explants per bottle, then place in MSB containing 6-BA and KT or KT and IBA 5 The culture medium was cultured for 7 days, the culture temperature was 23-27°C, the light intensity was 3000 lx, the light time was 16 h / day, the concentration of 6-BA was 0.8-1.2mg / L, and the concentration of KT was 0.02-2mg / L, the concentration of IBA is 0.15-0.25mg / L.

[0050] When the concentration of TDZ in the current treatment was 0.01mg / L, the effects of the combination of 6-BA and KT and the combination of KT and IBA on the induction of adventitious buds are shown in Table 3.

[0051] table 3

[0052]

[0053] When the pretreatment was TDZ 0.01 mg / L,...

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Abstract

The invention discloses an establishing method for an efficient regeneration system for multiple-genotype cowpeas. The method comprises the following steps: pretreatment, induction of adventitious buds, subculturing, adventitious bud elongating, rooting culture, seedling hardening and transplanting. In the pretreatment process, 6-BA with the concentration of 1-4 mg / L or TDZ with the concentration of 0.001-0.1 mg / L is used as a plant growth regulator; the induction process of adventitious buds, a composition of 6-BA and KT or a composition of KT and IBA is used as a plant growth regulator, wherein the concentration of the 6-BA is 0.8-1.2 mg / L, the concentration of the KT is 0.02-2 mg / L, and the concentration of the IBA is 0.15-0.25 mg / L; in the adventitious bud elongating process, 6-BA and IBA are used as a plant growth regulator, wherein the concentration of the 6-BA is 0.5 mg / L, and the concentration of the IBA is 0.1-0.3 mg / L. The efficient regeneration system is not only suitable for regeneration culture of the multiple- genotype cowpeas, but also has the advantages of high induction rate, multiple induced adventitious buds, long adventitious buds, high dry weight of adventitious buds, easy implementation conditions and has a good promotion prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for establishing a multi-genotype cowpea high-efficiency regeneration system. Background technique [0002] Cowpea is an important vegetable crop. It has a long history of cultivation in my country. It is cultivated except in alpine regions such as Tibet, and it is cultivated more in southern provinces and cities. Cowpea has high nutritional value. Its seeds are rich in protein (accounting for 25% of the total dry matter), vitamins, folic acid, potassium, magnesium, iron and other mineral elements, and also contain lysine and tryptophan necessary for the human body. [0003] Traditional cowpea cultivars have low yields, and high susceptibility to insect and fungal diseases is a major factor contributing to their low yields. There are 31 kinds of insect pests and more than 20 kinds of diseases during the field growth and storage of cowpea, and the insect pests alone can caus...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/00A01G1/00
Inventor 唐懿李焕秀孙国超汪志辉夏惠赖云松王迅吕秀兰涂利华黄志
Owner SICHUAN AGRI UNIV
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