A method for inducing and cultivating hybrid liquidambar tetraploid

A tetraploid and hybrid technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as induced breeding and reporting of allotetraploid of Liquidambar genus, and achieve improvement of tetraploid Induction rate, high ratio, good effect of plant growth

Active Publication Date: 2021-12-24
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] At present, there is no report on the technique of induction breeding of liquidambar allotetraploid

Method used

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  • A method for inducing and cultivating hybrid liquidambar tetraploid
  • A method for inducing and cultivating hybrid liquidambar tetraploid
  • A method for inducing and cultivating hybrid liquidambar tetraploid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] 1. Test materials

[0087] 1. Obtained from hybrid sweetgum seeds

[0088] The present invention uses the sweetgum tree and the sweetgum tree of North America that are well-grown, mature, healthy, and free from insect damage in Shanghai Chenshan Botanical Garden (Songjiang District, Shanghai, longitude: 121.22, latitude: 31.03).

[0089] In mid-March 2016 (10‐15), unopened flower branches of liquidambar formosana were collected, and then the flower branches were hydroponically cultured. After 3‐5 days, the water content of the stamen flower flower dropped and cracked, releasing gold. Yellow pollen, collect the pollen of the liquidambar tree, and then store it in a test tube containing a desiccant at low temperature;

[0090] At the end of March, 2016 (20-30), the female inflorescence and the male inflorescence can be completely separated, and the bracts on the male inflorescence have not fallen off. 3-5 days before the male flower looses powder, remove the male inflore...

Embodiment 2

[0117] Embodiment 2 prepares liquidambar aseptic seedling

[0118] 1. Sterilization of hybrid sweetgum seeds

[0119] Select plump and healthy seeds that are stored dry at 4°C, and disinfect them under an ultra-clean workbench, that is, first put the seeds into 75% alcohol (v / v) for 30-60s (preferably 45s (seconds) ), wash 1-2 times with sterile water, then put the seeds into 2% sodium hypochlorite solution (w / w) and soak them for 6-10min (preferably 8min), then clean them several times with sterile water (preferably 3 -5 times); then the hybrid sweetgum seeds are placed on sterile dry filter paper to dry the seed surface moisture to obtain sterilized hybrid sweetgum seeds (350);

[0120] 2. Seed germination treatment

[0121]Place the sterilized hybrid sweetgum seeds in the seed germination medium (WPM basic medium+sucrose 30g / L+agar 2g / L+Bilicon 4g / L, pH5.8-5.9), carry out the germination culture of seeds, 4 After -8 days, the seeds began to germinate. After 15 days, the ...

Embodiment 3

[0122] Embodiment 3 prepares aseptic explant

[0123] 1. After the seeds are germinated and cultivated for about 2 months (60-65 days), select 3 vigorously grown hybrid liquidambar aseptic seedlings (i.e. 3 genotype hybrid liquidambar aseptic seedlings named as Z1, Z2, Z3 genotype hybrid sweetgum sweet aseptic seedling), and 3 strains of hybrid sweetgum sweet aseptic seedlings are pruned to leave 2 blades at the top respectively, and the 2 blades at the top are cut in half, long 1.5-2.5cm (preferably 2cm ), and then inoculate them in the rooting medium (WPM medium + IBA 2.0mg / L + NAA 0.1mg / L + sucrose 30g / L + agar 2g / L + beilicon 4g / L, pH 5.8‐5.9) , carry out rooting culture, wherein, the culture condition of rooting culture of sweetgum seedling section is as follows: culture temperature is 25 ± 2 ℃, and light is 2000lx, and photoperiod is 16h light / 8h dark; After -15 days), the plant begins to take root, obtains respectively the rooted sweetgum seedling section (ie Z1, Z2, Z...

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Abstract

The invention discloses a method for inducing and cultivating hybrid liquidambar tetraploids, which comprises the steps of carrying out pre-differentiation culture treatment on the leaves and petioles of the isolated aseptic plants of the hybrid liquid sweetgum respectively, and then treating the pre-differentiated cultured The leaves and petioles were induced by colchicine respectively. The method of the present invention has a high induction rate of the hybrid liquidambar tetraploid, and a high rate of obtaining the hybrid liquidambar tetraploid, and adopts the method of the present invention to induce and obtain the hybrid liquidambar artificial allotetraploid for the first time, and the number of two heterologous chromosomes Being copied, it has both hybridization effect and ploidy effect, and can produce variation to a greater extent, which provides the possibility to further improve the genetic gain of liquidambar and the selection of improved varieties.

Description

technical field [0001] The invention relates to a method for inducing and cultivating plant polyploids, in particular to a method for inducing and cultivating tetraploids of hybrid sweetgum plants, and belongs to the technical field of cell engineering mutation breeding in the forestry industry. Background technique [0002] Sweetgum (Liquidambar spp) has strong adaptability, fast growth, early forest formation, tall tree body, straight trunk, broad crown, and beautiful leaf color. It is a veritable multi-purpose tree species. Liquidambar styraciflua and Chinese sweetgum (L.formosana) are the most widely used. Sweetgum sweetgum is widely distributed in the southern United States and the high-altitude mountainous areas from Mexico, Central America to Honduras. It is one of the most important commercial broad-leaved tree species in the United States. It can be used for wood, paper, plywood, fuel rods, etc.; for the United States Outstanding contribution to forestry production...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/08A01H4/00
CPCA01H1/02A01H1/08A01H4/001A01H4/008
Inventor 张金凤张炎赵健王泽伟范英明康向阳胡冬梅
Owner BEIJING FORESTRY UNIVERSITY
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