A method for establishing a high-efficiency regeneration system of cowpea suitable for multiple genotypes
A high-efficiency regeneration, multi-genotype technology, applied in the biological field, achieves the effects of high dry weight of adventitious buds, simple implementation steps, and great promotion prospects
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Embodiment 1
[0036] Select uniform and plump cowpea seeds, soak them in 75% alcohol for 1 minute, then soak them in 0.1% mercuric acid for 5 minutes, rinse them with sterile water for 5 times, and put them in a petri dish Inoculate the seeds on the culture medium with sterilized tweezers, and inoculate 10 seeds per bottle. Place cowpea seeds in MSBs containing 6-BA or TDZ 5 The culture medium was cultured for 5 days, the culture temperature was 25°C, the light intensity was 3000 lx, and the light time was 16 h / day. Then in the MSB containing 6-BA and KT or containing KT and IBA 5The culture medium was cultured for 7 days, the culture temperature was 23-27°C, the light intensity was 3000 lx, and the light time was 16 h / day. Six experimental groups were established. In the first experimental group, 6-BA and KT combination, the concentration of 6-BA is 0.8mg / L, the concentration of KT is 0.02mg / L, in the second experimental group, the combination of 6-BA and KT is used, the concentration of...
Embodiment 2
[0042] When the concentration of 6-BA in the current treatment is 3 mg / L, after the pretreatment, the cotyledon node with one cotyledon is cut from the aseptic seedling after the pretreatment, the node is 1 mm away from the epicotyl, and 5 mm away from the hypocotyl, Then inoculate on adventitious bud induction medium, inoculate 5-6 explants per bottle, and then put into MSB containing 6-BA and KT combination or KT and IBA combination 5 The culture medium was cultured for 7 days, the culture temperature was 23-27°C, the light intensity was 3000 lx, the light time was 16 h / day, the concentration of 6-BA was 0.8-1.2mg / L, and the concentration of KT was 0.02-2mg / L, the concentration of IBA is 0.15-0.25mg / L.
[0043] When the concentration of 6-BA in the current treatment was 3mg / L, the effects of the combination of 6-BA and KT and the combination of KT and IBA on the induction of adventitious buds are shown in Table 2.
[0044] Table 2
[0045]
[0046] It can be seen from T...
Embodiment 3
[0049] When the concentration of TDZ in the current treatment is 0.01mg / L, after the pretreatment, the cotyledon node with one cotyledon is cut from the aseptic seedling after the pretreatment, and the node is 0.5 mm away from the epicotyl, 5 mm away from the hypocotyl, and then Inoculate on Adventitious Bud Induction Medium, inoculate 5-6 explants per bottle, then place in MSB containing 6-BA and KT or KT and IBA 5 The culture medium was cultured for 7 days, the culture temperature was 23-27°C, the light intensity was 3000 lx, the light time was 16 h / day, the concentration of 6-BA was 0.8-1.2mg / L, and the concentration of KT was 0.02-2mg / L, the concentration of IBA is 0.15-0.25mg / L.
[0050] When the concentration of TDZ in the current treatment was 0.01mg / L, the effects of the combination of 6-BA and KT and the combination of KT and IBA on the induction of adventitious buds are shown in Table 3.
[0051] table 3
[0052]
[0053] When the pretreatment was TDZ 0.01 mg / L,...
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