Method for acquiring regeneration plants by adventitious bud inducement of camellia oleifera radicles
A technology for plant regeneration and bud induction, which is applied in the fields of botanical equipment and methods, plant regeneration, and horticultural methods.
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[0036] Do the following in order:
[0037] 1. Select mature raw camellia oleifera seeds of the same year, remove the seed coat, rinse the seeds with tap water for 3-5 minutes, then soak for 10-15 hours, change the water 2-3 times in the middle, and then use 75% of the water in the ultra-clean workbench Soak in alcohol for 20-30s, rinse with sterile water for 3-5 times, and then use 0.1% HgCl 2 Disinfect for 3-5 minutes, wash 5-6 times with sterile water, cut off most of the endosperm, inoculate the seed embryo with endosperm in WPM basic medium, culture in the dark for 2-3 days, then transfer to light for 20- Sterile vaccines can be obtained in 25 days; the culture temperature is 26±1°C, the light intensity is 2100-2200lx, and the light time is 12-14h / d.
[0038] Table 1 Effect of different disinfection treatment methods on the disinfection effect of Camellia oleifera germ
[0039] 75% alcohol 0.1% mercury liter pollution rate browning rate 0 2 83.23 0...
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