Tissue culture method of dracocephalum rupestre hance and application thereof

A tissue culture and culture medium technology, applied in the field of plant tissue culture, can solve the problems of low bud induction rate and rooting rate, many types of hormones, difficulty in differentiation and budding, etc., and achieves an increase in the induction rate and rooting rate of adventitious buds, and fewer types of hormones. , the effect of simple operation

Active Publication Date: 2020-08-21
MINZU UNIVERSITY OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there are very few research reports on the tissue culture method of Cinnamomum chinensis, and most of the reported studies use the stems of Cinnamomum spp. There are many types of hormones, high cost, and complicated cultivation process.

Method used

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  • Tissue culture method of dracocephalum rupestre hance and application thereof
  • Tissue culture method of dracocephalum rupestre hance and application thereof
  • Tissue culture method of dracocephalum rupestre hance and application thereof

Examples

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Embodiment 1

[0047] Embodiment 1, the tissue culture method of chrysanthemum

[0048] 1. Disinfection method of chrysanthemum seeds and acquisition of aseptic seedlings

[0049] 1. Put the collected chrysanthemum seeds in a small beaker, soak them in distilled water, change the distilled water every 8-10 hours, and wash off the fluff on the surface with gauze after 24 hours until the surface is not sticky.

[0050] 2. Transfer the seeds obtained in step 1 to the ultra-clean workbench, soak them in 75% alcohol for 30s, then rinse them with sterile water for 3 times, and then use 10-12% (volume fraction) of H 2 o 2 The solution was disinfected for 15-30 minutes, and finally rinsed with sterile water 4-5 times. According to H 2 o 2 Different concentrations and different disinfection times are divided into the following treatment groups:

[0051] Treatment 1: 10% (volume fraction) of H 2 o 2 The solution was disinfected for 15 minutes.

[0052] Treatment 2: 10% (volume fraction) of H ...

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Abstract

The invention discloses a tissue culture method of dracocephalum rupestre hance and application thereof. The tissue culture method of the dracocephalum rupestre hance comprises the following steps: (1) culturing dracocephalum rupestre hance seeds after disinfection in sterile condition so as to obtain sterile seedlings; 2) culturing terminal buds of the sterile seedlings as explants in an inducingmedium so as to obtain cluster buds; and 3) culturing single buds taken from the cluster buds in a rooting medium so as to obtain regenerated dracocephalum rupestre hance seedlings. The tissue culture method of the dracocephalum rupestre hance adopts the terminal buds of the sterile seedlings as the explants; and the explants are cultured in the inducting medium containing 6-benzylaminoadenine (6-BA) or thidiazuron (TDZ) so as to carry out adventitious bud culture. The tissue culture method of the dracocephalum rupestre hance disclosed by the invention is simple, fast, improved in adventitious bud induction rate, and capable of ensuring quick breeding so as to obtain regenerated dracocephalum rupestre hance plants.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture method and an application of C. cichlidum, in particular to a method for rapidly obtaining regenerated ciconia cinerea plants by utilizing terminal buds of Ciconia aseptic seedlings and its application. Background technique [0002] Dracocephalum rupestre Hance is a plant of the genus Dracocephalum Linn. in the family Lamiaceae, also known as Maojiancao, commonly known as Maojian. Perennial herb, plant height 15-42cm, leaves triangular-ovate, with circular serrations on the edge; flowering period from July to September, in whorls, purple-blue and large flowers, available for viewing. The whole plant has aroma and can be used as a substitute for tea. In my country, Yanqinglan is mainly distributed in grasslands at an altitude of 650-3100m, and is produced in Inner Mongolia, Shanxi, Hebei and other places. [0003] Yanqinglan is also know...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王俊丽王晓东黄航君李依陆旭马伟伟杜嘉琳耿兆鹏卢娇
Owner MINZU UNIVERSITY OF CHINA
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