Method for tissue culture and rapid propagation of polygonatum filipes

A tissue culture, Polygonatum chinensis technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, to achieve the effects of facilitating industrial production, shortening the occurrence time, and low pollution rate

Active Publication Date: 2017-06-27
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture and rapid propagation of Polygonatum long stems has not been reported yet

Method used

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  • Method for tissue culture and rapid propagation of polygonatum filipes
  • Method for tissue culture and rapid propagation of polygonatum filipes
  • Method for tissue culture and rapid propagation of polygonatum filipes

Examples

Experimental program
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Embodiment 1

[0029] A method for tissue culture and rapid propagation of Rhizoma Polygonatum, comprising the following steps in turn:

[0030] (1) Disinfection of Polygonatum long-stemmed flower buds

[0031] In May of that year, take the flower buds of Polygonatum long-stemmed that have not yet bloomed, put them in the refrigerator at 4°C for 12-24 hours, add an appropriate amount of detergent solution to soak for 2 minutes, wash them with running water for 0.5-1.0 hours, and put them in the ultra-clean bench Turn the stem section into 70% alcohol solution and soak for 1min, rinse it with sterile water for 3 times, and dip it in 5 drops of Tween-80, the mass fraction is 0.1% HgCl 2 The solution is soaked and disinfected for 9-10 minutes, and then fully soaked with sterile water for 3 times to obtain the sterilized explant material, and the success rate of disinfection can reach more than 90%.

[0032] (2) Induction of clustered buds

[0033] Put the explant material obtained in the prev...

Embodiment 2

[0041] The difference between this example and Example 1 lies in step (1): Disinfection of Polygonatum long-stemmed flower buds: In May of that year, take the flower buds of Polygonatum long-stemmed that have not yet bloomed, put them in the refrigerator at 4°C for 0-48h, take them out and add them Soak in an appropriate amount of detergent solution for 2 minutes, rinse with running water for 0.5-1.0 hours, then transfer the stem segment to 70% alcohol solution and soak for 1 minute in an ultra-clean bench, rinse with sterile water for 3 times, immerse in 5 drops of Drip Tween-80, HgCl with a mass fraction of 0.1% 2 The solution is soaked and disinfected for 9-10 minutes, and then fully soaked with sterile water for 3 times to obtain the sterilized explant material. It can be seen from the statistical results 30 days after inoculation that the pollution rate obtained by using flower buds as explants for disinfection is far lower than that of using rhizomes as explants, and the...

Embodiment 3

[0045] The difference between this embodiment and Example 1 is that step (2): the induction of clustered buds: put the explant material obtained in the previous step on sterile filter paper, cut off the head and tail, remove the stamens and pistils, and cut the petals into 2cm 2 Size, in the MS basic medium with TDZ 0-2.0mg / L, NAA 0-1.0mg / L, AC 0-2.0g / L, the amount of sucrose in the medium is 30g / L, agar The amount is 8.0g / L, the pH is 5.8-6.0, and the medium has been sterilized at 121°C for 20min, the same below. The petal-connected culture medium was first cultured in the dark for 0-7 days, and the culture temperature was 28±2°C; then it was transferred to light culture, and the culture conditions were as follows: the culture temperature was (28±2)°C, and the light time was 14h light / 10h dark, light intensity 30-40μmol / (m 2 s); after 30d, statistics found that; the different treatments of TDZ concentration showed that low concentration TDZ (0.1mg / L) had lower adventitious...

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Abstract

The invention belongs to the technical field of plant culture and particularly relates to a method for tissue culture and rapid propagation of polygonatum filipes. The method for tissue culture and rapid propagation of polygonatum filipes comprises the following steps that (1), polygonatum filipes flower buds are disinfected and inoculated; (2), cluster buds are induced; (3), adventitious buds are proliferated; (4), rooting culture of regenerated plants is performed; (5) acclimatization and transplanting are performed. By adopting the method and compared with reported literatures, the preprocessing time can be shortened, the explant pollution rate is low, the adventitious bud generating time is shortened, accordingly regenerated polygonatum filipes plants are obtained more quickly, and industrial production is promoted. By adopting the method, the adventitious bud inducing rate of the cluster buds of the polygonatum filipes is as high as 90.0%, the number of the adventitious buds is 8.4, the adventitious bud proliferation rate can be up to 7.7, and the survival rate of transplanted plants can be up to 95% or above.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for tissue culture and rapid propagation of Polygonatum long-stalked. Background technique [0002] Polygonatum filipes Merr. is a perennial herb of Liliaceae Polygonatum, distributed in Jiangsu, Anhui, Zhejiang, Jiangxi, Hunan, Fujian, Guangdong (northern), and grows in the shade of forests, shrubs or grass slopes. In wet and fertile soil, it is grown in large quantities because of its rhizomes and is used as medicine in the local area, and it is also edible. At present, seeds and rhizomes are used for the propagation of Polygonatum long-stemmed Rhizoma in production, mainly based on rhizomes, which have the disadvantages of low reproduction coefficient and long propagation cycle, so establishing a tissue culture and rapid propagation system for Polygonatum long-stemmed Another effective way for its industrialized production. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 孙骏威赵进周荣鑫
Owner CHINA JILIANG UNIV
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