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Method for building regenerative system of kostelezkya virginica

A mallow and system technology, applied in the field of establishing a regeneration system using mature embryos of seaside mallow, can solve the problems of not getting tissue cultured seedlings

Inactive Publication Date: 2008-05-28
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research group conducted experiments according to the method provided by Deborah et al., but did not obtain tissue cultured plantlets from callus induced by stem segments according to the reported results.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for establishing the regeneration system of seaside mallow by using mature embryos of seaside mallow as explants for in vitro culture mainly includes the acquisition of explants, induction of callus, subculture of callus, induction of adventitious buds, root The induction and transplanting and hardening of aseptic seedlings, the specific steps are:

[0026] 1. Obtaining and surface disinfection of mature embryos of seashore mallow as explants

[0027] (1) Take several mature seashore mallow seeds, soak them in concentrated sulfuric acid for 1 hour, and soften the seed coat;

[0028] (2) Rinse the seashore mallow seeds soaked in concentrated sulfuric acid with double distilled water for more than 5 times, carefully peel off the seed skin with tweezers and a scalpel, and a complete seed embryo appears;

[0029] (3) Excision of cotyledon and radicle, leaving hypocotyl part as explant;

[0030] (4) Surface disinfection of explants: the hypocotyls used as explan...

Embodiment 2

[0041] The method for establishing the regeneration system of seashore mallow by using mature embryos of seashore mallow as explants described in this example, the specific operation steps are the same as in Example 1, but the callus used in step 2 is induced The medium is MS basal medium containing 1.0mg / L 2,4-D, 30g / L sucrose and 8g / L agar, and the callus induction rate is 75%.

Embodiment 3

[0043] The method for establishing the regeneration system of seashore mallow by using mature embryos of seashore mallow as explants described in this example, the specific operation steps are the same as in Example 1, but the callus used in step 2 is induced The medium is MS basal medium containing 1.5mg / L 2,4-D, 30g / L sucrose and 8g / L agar, and the callus induction rate is 86.8%.

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Abstract

The invention relates to a method for buildsing-up a regeneration system for beach mallow, using beach mallow mature embryos as explants, comprising following steps: (1)explants surface sterilization, (2)tissue culture, with MS basal culture medium containing 0.5-2.0mg / L of 2,4-dichlorophe noxyacetic acid,(3)tissue subculture, with MS basal culture medium containing 0.3mg / L of heteroauxin and 0.3mg / L of zeatin, (4)adventitious bud culture, with MS or 1 / 2 MS basal culture medium containing 0.1-0.2mg / L of heteroauxin and 0.3-0.5mg / L of zeatin, (5) rooting culture, with MS basal culture medium free of hormones, (6)obtusifolia seedlings transplanting and hardening. The method is provided with a tissue culture rate, a adventitious bud culture rate, a rooting rate up to 86.8%,64.2% and 95.2% respectively, and the survival rate of regenerating seedlings up to 80%.

Description

technical field [0001] The invention relates to plant regeneration through tissue culture technology, in particular to a method for establishing a regeneration system using mature embryos of seaside mallow. Background technique [0002] Seaside mallow (Kostelezkya virginica L.Presl.) is one of the salt-tolerant plants introduced from abroad in recent years in my country (Yin Zengfang et al., Journal of Plant Resources and Environment, 2006, 15(1): 14-17), native to the United States In the saline marsh area along the eastern coast, the salt content of the growth substrate can be as high as 2.5%, and it is a perennial herb suitable for growing in coastal mudflat areas. Seashore mallow seeds are rich in fat and protein, and the seed oil can be used as power fuel, which is one of the important plant resources for biodiesel (Yin Jinlai et al., Jiangsu Agricultural Science, 2000 (6): 29-31; Ruan Chengjiang, Dalian University for Nationalities Journal of the Chinese Academy of Sci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 阮成江金华郑熙单莹
Owner DALIAN NATIONALITIES UNIVERSITY
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