Inducing method of Buchloe dactyloides callus
A technology of callus induction and callus, which is applied in the field of callus induction of bison grass, can solve the problems of callus browning regeneration rate, and achieve the effect of improving callus induction rate, reducing difficulty and eliminating interference
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Embodiment 1
[0027] Example 1 Optimization of 2,4-D concentration in callus induction medium
[0028] 1. Pretreatment of seeds: taking out seeds from glumes;
[0029] 2. Disinfection of explants: first put the washed seeds into a sterile container, sterilize with 70% alcohol for 60 seconds in a sterile workbench, then sterilize with 7-10% sodium hypochlorite solution for 30 minutes, and use sterile Rinse with water 4-5 times before use.
[0030] 3. Germination of seeds: first configure MS medium without hormones, sterilize it and pour it into a large petri dish to cool for later use, and place the sterilized seeds in a large petri dish with hormone-free MS medium. Afterwards, the petri dish was sealed and placed in a light incubator for dark cultivation for 2-3 days.
[0031] 4. Induction of mature embryo callus: select seeds that have germinated and grown radicles from the petri dish, and transfer them to the callus induction medium for callus induction (see figure 1 , figure 2 ). T...
Embodiment 2
[0034] Example 2 Optimization of Germination of Seeds
[0035] 1. Pretreatment of seeds: taking out seeds from glumes;
[0036] 2. Disinfection of explants: first put the washed seeds into a sterile container, sterilize with 70% alcohol for 60 seconds in a sterile workbench, then sterilize with 7-10% sodium hypochlorite solution for 30 minutes, and use sterile Rinse with water 4-5 times before use.
[0037]3. Germination of seeds: first spread double-layer filter paper in the petri dish, after autoclaving for 20 minutes, place it in a sterile operating table, moisten it with sterile water, place the sterilized seeds on the sterile filter paper, and Add some sterile water to the Petri dish to keep the seeds hydrated for germination. Afterwards, the petri dish was sealed and placed in a light incubator for dark cultivation.
[0038] 4. Induction of mature embryo callus: select seeds that have germinated and grown radicles from the petri dish, and transfer them to the callus i...
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