A Method for Effectively Preserving Embryogenic Callus of Calamus Iris

A technique for embryogenic callus and callus, which is applied in the field of effectively preserving embryogenic callus of A. calamus, can solve the problems of difficulty in later preservation and proliferation, poor embryogenicity of callus, loss of plant mother, etc. Differentiation and differentiation, easy operation, good effect of callus embryo

Inactive Publication Date: 2017-06-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using young leaves and flower organs as explants, the induction efficiency is low, and the callus obtained is poor in embryonicity, and it is not easy to preserve and proliferate in the later stage. , carrying a lot of germs, it is difficult to sterilize in the tissue culture process, the pollution rate is high, and it needs to be at the expense of the loss of the plant mother

Method used

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  • A Method for Effectively Preserving Embryogenic Callus of Calamus Iris
  • A Method for Effectively Preserving Embryogenic Callus of Calamus Iris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Step 1: Material collection:

[0033] Take the well-developed capsule (7-10cm in length) 50 days after the flowering stage of Iris japonica, put it in a sealed bag and refrigerate it in a refrigerator at 4°C for 4 days, then soak it in tap water with detergent and a small amount of TWEEN-20 for about 20 minutes , and then rinsed under running water for 40 minutes, and then sterilized on the ultra-clean workbench; after the capsules were immersed in an alcohol solution with a volume concentration of 70%, they were burned on the flame of an alcohol lamp until the alcohol on the surface of the capsules was completely volatilized.

[0034] Step 2: callus induction culture:

[0035] Place the sterilized capsules in step 1 on the high-temperature sterilized filter paper, cut them along the longitudinal edge of the fruit with tweezers and a scalpel, take out the full-grained seeds and put them on the sterile filter paper, peel off the white tender embryos and inoculate On the...

Embodiment 2

[0042] Step 1: Material collection:

[0043] Take the well-developed capsule (7-10cm in length) 50 days after the flowering stage of Iris japonica, put it in a sealed bag and refrigerate it in a refrigerator at 4°C for 4 days, then soak it in tap water with detergent and a small amount of TWEEN-20 for about 20 minutes , and then rinsed under running water for 40 minutes, and then sterilized on the ultra-clean workbench; after the capsules were immersed in an alcohol solution with a volume concentration of 70%, they were burned on the flame of an alcohol lamp until the alcohol on the surface of the capsules was completely volatilized.

[0044] Step 2: callus induction culture:

[0045] Place the sterilized capsules in step 1 on the high-temperature sterilized filter paper, cut them along the longitudinal edge of the fruit with tweezers and a scalpel, take out the full-grained seeds and put them on the sterile filter paper, peel off the white tender embryos and inoculate On the...

Embodiment 3

[0051] Step 1: Material collection:

[0052] Take the well-developed capsule (7-10cm in length) 50 days after the flowering stage of Iris japonica, put it in a sealed bag and refrigerate it in a refrigerator at 4°C for 4 days, then soak it in tap water with detergent and a small amount of TWEEN-20 for about 20 minutes , and then rinsed under running water for 40 minutes, and then sterilized on the ultra-clean workbench; after the capsules were immersed in an alcohol solution with a volume concentration of 70%, they were burned on the flame of an alcohol lamp until the alcohol on the surface of the capsules was completely volatilized.

[0053] Step 2: callus induction culture:

[0054] Place the sterilized capsules in step 1 on the high-temperature sterilized filter paper, cut them along the longitudinal edge of the fruit with tweezers and a scalpel, take out the full-grained seeds and put them on the sterile filter paper, peel off the white tender embryos and inoculate On the...

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Abstract

The invention relates to the technical field of cell engineering tissue culture, and aims to provide a method for effectively preserving embryogenic callus of Acorus calamus. The method for effectively preserving the embryogenic callus of Acorus iris includes the steps of material collection, callus induction culture, screening and preservation of the embryogenic callus. The invention uses the young embryos of calamus calamus as explants, which has extremely low pollution rate, high callus induction rate, good embryogenicity of callus, and high reproduction coefficient, and at the same time solves the problem of easy browning and differentiation in the preservation of calamus calamus callus Serious problems, strong practicability, easy operation and good promotion.

Description

technical field [0001] The invention relates to the technical field of cell engineering tissue culture, in particular to a method for effectively preserving embryogenic callus of Acorus calamus. Background technique [0002] Iris is a perennial wet or emergent perennial plant of the genus Iris in the family Iridaceae. It is the favorite among aquatic flowers, with beautiful flowers, green leaves and high ornamental value. In addition, Iris has strong resistance, can tolerate salt and alkali, and has a certain ability to absorb heavy metal ions and organic pollution in water bodies. With the acceleration of urban and rural waterscape construction, its use in urban waterscapes continues to increase. [0003] Although Iris has high ornamental value and ecological adaptability, it still has the disadvantages of single flower color and short flowering period of single flower. The directional transformation of plant traits through transgenic technology is a faster breeding method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 夏宜平李丹青王冠群李康马怡迪
Owner ZHEJIANG UNIV
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