31results about How to "Easy to differentiate" patented technology

The invention belongs to the technical field of forest creatures, and discloses a cultivation method for quickly obtaining lycium ruthenicum regeneration seedlings. The cultivation method comprises the following steps: disinfection of seeds, obtaining of germfree seedlings, induction of a callus, obtaining of adventitious buds and induction of roots. According to the cultivation method, the callus is directly obtained from the germfree seedlings of lycium ruthenicum, and the lycium ruthenicum regeneration seedlings are cultivated through a tissue culture method; compared with a conventional method for inducing the callus through leaves or stems of the seedlings, the cultivation method has the advantages that the tissue culture time is greatly shortened, and cultivation cost of the lycium ruthenicum is greatly reduced; meanwhile, the cultivation efficiency is effectively improved, and the transplanting survival rate of the regeneration seedlings reaches up to 98 percent. The lycium ruthenicum obtained through the cultivation method has the characteristics of being high in genetic stability, low in cost, short in seedling growing period, high in reproduction coefficient, and easy and convenient to operate.

The invention relates to prevention and treatment of bone diseases caused by glucocorticoid medicines. Concretely, the invention relates to application of Wnt signal pathway to prevention and treatment of bone diseases caused by glucocorticoid medicines. Expression of C/EBPalpha factor is down regulated at a later period that BMP2 induces a mouse mesenchymal stem cell line C3H10T1/2 to be differentiated into osteoblast, and overexpression of the factor can inhibit differentiation of osteoblast. The reason causing down-regulated expression of C/EBPalpha is that distal promoters (-1286bp/-1056bp) of C/EBPalpha are subjected to hypermethylation at the differentiation later-period of osteoblast and are silenced. Interruption of the methylation process promotes high expression of C/EBPalpha and leads to osteogenic/adipogenic differentiation unbalance. A Dex-induced osteoporosis model proves that Wnt/beta-catenin signal pathway has important regulation and control effects on expression and methylation of C/EBPalpha in differentiation of osteoblast. A stimulant/activator of the pathway is capable of rescuing osteogenic/adipogenic differentiation unbalance caused by Dex.

The invention discloses a propagation method of vernonia amygdalina. The propagation method is characterized in that a proliferation bud of the vernonia amygdalina is pre-cultured in a rooting culture medium to obtain an aseptic seedling, then the aseptic seedling is cut to be cultured in a matrix, and after the aseptic seedling is rooted and has new leaves, the aseptic seedling is then transplanted into a flowerpot. The vernonia amygdalina is propagated by combining a tissue culture technology and the cutting technology, so that complementary advantages can be realized, and the pre-cultured and non-rooted aseptic seedling is cut into a mixed matrix of peat soil, river sand and vermiculite to be rooted, and the difficulty that the culture medium is washed, so that the seedling is likely to infect harmful bacteria can be overcome, and the survival rate is increased; a great amount of cutting propagation materials can be provided all year round by adopting the tissue culture technology, and the mass production and all-year production requirements of seedlings can be met.

The invention relates to a tissue culture propagation method of Polygonatum multiflorum, comprising the steps of in an aseptic environment, collecting embryo from mature seeds of Polygonatum multiflorum by peeling, and sterilizing the embryo; inoculating the sterilized embryo to a callus induction medium, and performing light-free culture for 7-12 days so that the embryo expands to form a callus;cutting the callus into small pieces, inoculating the pieces to a callus subculture medium, and culturing for 18-25 days under light intensity of 800-1000 lux and daily lighting time of 10-12 h; cutting the callus subjected to subculture in step S3 into small pieces, inoculating the pieces to a sprouting medium, and culturing for 28-35 days under the light intensity of 1200-1800 lux and daily lighting time of 10-12 h, and inducing to obtain cluster buds; inoculating the robust single ones of the cluster buds to a rooting medium, and performing rooting culturing. The method herein helps greatlyreduce pollution rate of a tissue culture process, and improve propagation efficiency and seedling quality.

The invention belongs to the technical field of composite scaffolds and discloses a composite scaffold for improving gaps and pores to promote the cell adhesion rate and a preparation method. A channel pore and a spherical pore of the composite scaffold are 150mum-650mum and 3mum-15mum respectively; the preparation method comprises the following steps: obtaining a CS/HA (chitosan/hydroxyapatite) scaffold with pores and spherical pores by adopting an in situ hybridization technology and a freeze-drying method; connecting CS and RGD (arginine-glycin-aspartate) peptides with hydrogen bonds, and modifying the RGD (arginine-glycin-aspartate) peptides on the surface of a porous channel of the CS/HA scaffold with a static self-assembly method, so as to prepare a three-dimensional porous CS/HA scaffold with efficient adherent cells containing the RGD peptides. The scaffold has better cytocompatibility; through composition with RGD, the functions of attraction, induction and growth control of the three-dimensional porous CS/HA scaffold on cells are obviously enhanced; a physical absorption method and a chemical fixation method are combined in the preparation method, so that the material hasbetter adsorbability and stability.

The invention discloses an in-vitro inducing method for a high-expression Nurrl gene of a human mesenchymal stem cell, comprising the following step of inducing the high-expression Nurrl gene of the human mesenchymal stem cell in a DMEM (Dulbecco's Modified Eagle's Medium)/F-12 cultivation system in which RA (Retinoic Acid), CAMP (Cyclic Adenosine Monophosphate) and SHH (Sonic Hedgehog Homolog) are added. By using the in-vitro inducing method instead of a gene modification method, the expression of the Nurrl gene of the mesenchymal stem cell can be remarkably improved (about 23 times that before induction), the Nurrl gene of the mesenchymal stem cell is enabled to differentiate towards the dopaminergic neuron more easily, risks brought by gene modification is avoided, and a more ideal seed cell is provided for the clinical. Compared with an embryonic stem cell and a nerve stem cell, the mesenchymal stem cell is convenient to obtain materials, easy for in-vitro amplification, low in immunogenicity and capable of meeting the requirement of ethics.

A method of associating wireless devices with a wireless medical body area network, MBAN, where the wireless MBAN comprises at least one host is provided. The method comprises activating the host to search for wireless devices in range; displaying a list on the host of available wireless devices in range, wherein displaying the list comprises displaying each wireless device on the list with a unique representation on the list and the same unique representation on the wireless device itself; selecting a wireless device on the list; and associating the selected wireless device on the list with the host.

The invention relates to a method for increasing memory stem cell phenotypes by using culture medium cultured CD19 CART cells containing IL7 and IL-15 cell factors, and belongs to the field of biotechnology. Specifically, the used IL7 and IL-15 culture medium cultivated CD19CART cells are more easily to be differentiated into memory stem cell phenotypes, and IL7 and IL-15 preteatment used for T cells indicates that the memory T cell function and CAR-T cell anti-tumor activity can be improved.

The invention discloses a culture medium and a method for improving genetic transformation efficiency of tobacco Honghuadajinyuan. The culture medium comprises a co-culture medium and a selective culture medium; the co-culture medium and the selective culture medium each comprise an MS basic culture medium, NAA and 6-BA, the final concentration of the NAA is 0.41 mg/L, and the concentration ratioof the NAA to the 6-BA is 1:5; the method comprises the following steps: preparing a tobacco Honghua Dajinyuan leaf disc; infecting with agrobacterium tumefaciens; carrying out selective culture; andcarrying out rooting culture. According to the method, the budding rate (differentiated buds/leaf discs) of tobacco calluses reaches 127%, and the rooting rate (rooted seedlings/differentiated buds) reaches 66%; compared with an original method (the budding rate is 62%, and the rooting rate is 10%), the budding rate is increased by about 2 times, and the rooting rate is increased by about 6 times;and the requirement for large-scale genetic transformation is met.

A method for co-culturing with nerve stem cells to inducing rASCs to be DA Neurons by Lmx1a mediation, belongs to a method for treating Parkinson's disease by inducing stem cells to replacing defective Neuron cells. The method of the invention comprises steps of: building Lmx1a gene clone, recombining plasmid, building, packaging and recombining adenovirus Ad-Lmx1a, and co-culturing rASCs and NSCs which are infected by Ad-Lmx1a to differentiating them to be DA Neurons; and culturing rASCs and NSCs passage cells. Protein marker for expressing dopaminergic neuron cells can be differentiated from inducted neuron-like cells in the invention, and the inducted cells can transmit electric signal or secrete neurotransmitters, to prove that the inducted cells are the Neuron cells.

The invention relates to a Chinese narcissus callus preservation and tissue culture method. The method comprises the steps of material selection, induction culture and callus preservation. When a Chinese narcissus callus preservation and culture medium provided by the invention is put into use, the reproduction coefficient of Chinese narcissus callus can be 5.16 times, and the callus grows well, has a high reproduction speed and is free from browning and differentiation phenomena. According to the Chinese narcissus callus preservation and tissue culture method, the problems of difficult subculture and preservation, low reproduction coefficient, serious browning and differentiation and the like of the Chinese narcissus callus can be solved, the practicability is high and the generalization performance is good. The callus preserved by the method is easy to differentiate when being transferred to a differential medium, and can serve as an excellent material of a receptor for genetic transformation of Chinese narcissus.

The invention discloses a prawn ecological culture and polyculture method based on facility microalgae culture. The prawn ecological culture and polyculture method comprises the following steps of preparing corresponding microalgae and prawn seeds; then putting the microalgae and the prawn seeds into a culture pond for culture; carrying out feeding in the later culture process, and carrying out culture monitoring on the culture pond by means of a cleaning control system; and finally, continuously carrying out breeding. The cleaning control system comprises an algae recording unit, a data recording unit, a content detection unit, a single database, a data prediction unit, a single shrimp acquisition unit, a processor, a display unit, a suggestion generation unit and a management unit. By means of the cleaning control system, the number of the microalgae in the culture pond is monitored in real time, meanwhile, the growth condition of prawns, the number of culture days and the related feeding amount are combined to reasonably predict when the corresponding microalgae can reach the corresponding upper limit value, and according to the number of the microalgae reaching the upper limit, whether local targeted cleaning or integral cleaning is needed or not is judged; and differential treatment is facilitated.

The invention provides a rainwater collecting and recycling system. The system comprises a roof rainwater collecting device used for collecting roof rainwater and storing the roof rainwater in a roof water storage tank, a road surface rainwater collecting device used for collecting rainwater on the road surface and storing the rainwater in an underground water storage tank, a monitoring device used for monitoring the water level of the roof water storage tank, and a control device used for controlling a drain valve corresponding to the roof water storage tank to be opened under the condition that the monitoring device monitors that the water level in the roof water storage tank meets a preset condition. Under the condition that the monitoring device detects that the water level in the roof water storage tank does not meet the preset condition, the drain valve corresponding to the underground water storage tank is controlled to be opened, so that the rainwater is conveyed to the rainwater recycling device to be recycled.