Intermediate propagation method of vernonia amygdalina

Abstract

The invention discloses a propagation method of vernonia amygdalina. The propagation method is characterized in that a proliferation bud of the vernonia amygdalina is pre-cultured in a rooting culture medium to obtain an aseptic seedling, then the aseptic seedling is cut to be cultured in a matrix, and after the aseptic seedling is rooted and has new leaves, the aseptic seedling is then transplanted into a flowerpot. The vernonia amygdalina is propagated by combining a tissue culture technology and the cutting technology, so that complementary advantages can be realized, and the pre-cultured and non-rooted aseptic seedling is cut into a mixed matrix of peat soil, river sand and vermiculite to be rooted, and the difficulty that the culture medium is washed, so that the seedling is likely to infect harmful bacteria can be overcome, and the survival rate is increased; a great amount of cutting propagation materials can be provided all year round by adopting the tissue culture technology, and the mass production and all-year production requirements of seedlings can be met.

Description

technical field [0001] The invention relates to a propagation method of Vernonia amygdala, in particular to a rapid propagation method of Vernonia amygdala in combination with tissue culture and cutting techniques. Background technique [0002] Vernonia amygdalina Del., also known as Vernonia amygdalina Del., also known as Vernonia amygdalina, Apricot Vernonia, Miracle Tree, Bitter Tree, South Africa Tree, South Africa Leaf, Pintail Phoenix, etc., is a plant of the Asteraceae Vernonia, native to Africa, Most common in West Africa. At present, there are about 1,000 species of Vernonia plants in the Asteraceae family, most of which belong to herbs, woody vines or trees, and mainly grow in tropical regions. About 30 species have been found in my country, including Vernonia rugosa, Vernonia willow, Vernonia repellent, and Nocturnal oxen, etc. The main species are distributed in southwest to southeast, Taiwan, Xinjiang and other places. Vernonia amygdala is mostly a perennial t...

AI Technical Summary

Problems solved by technology

During the cultivation process of cutting technology, diseases will accumulate year by year, causing the propagation seedlings to degenerate; if tissue culture is adopted, when the rooted seedlings are taken out from the medium, the medium on the roots of the seedlings is not easy to clean, which is labor-intensive, and the medium is rich in nutrients. During the hardening and planting process, it is easy to be infected with germs and reduce the survival rate