Intermediate propagation method of vernonia amygdalina

A fast technology of Vernonia almond, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of difficult cleaning of the culture medium, degradation of breeding seedlings, lower survival rate, etc., so as to overcome the degradation of breeding seedlings and reduce the Diseases, the effect of reducing the survival rate

Active Publication Date: 2015-12-30
SHENZHEN TECHAND ECOLOGY & ENVIRONMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the cultivation process of cutting technology, diseases will accumulate year by year, causing the propagation seedlings to degenerate; if tissue culture is adopted, when the rooted seedlings are taken out from the medium, the medium on the roots of the seedlings is not easy to clean, which is labor-intensive, and the medium is rich in nutrients. During the hardening and planting process, it is easy to be infected with germs and reduce the survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Propagate Almond Vernonia by following these steps:

[0032] (1) Induction of explants: Stems were cut from Vernonia amygdala plants, which were growing robustly and free from diseases and insect pests, as explants, soaked in a diluted solution of detergent, and then cleaned the surface of the explants with a brush. Then rinse with running water for about 1 hour, soak in alcohol with a volume ratio of 75% for about 25 seconds on the ultra-clean workbench, rinse with sterile water for 3 times, then disinfect with 1g / L mercury chloride solution for about 9 minutes, rinse with sterile water After 3 times, inoculate in MS starting medium containing 1mg / L6-BA and 0.1mg / LNAA, culture temperature is 25°C, light intensity is 1500LX, light time is 13h per day;

[0033] (2) Subculture of induced buds: when the induced buds grow to 2~3cm, inoculate them into MS proliferation medium containing 1mg / L6-BA and 0.1mg / LNAA, the temperature is 25℃, the light intensity is 1500LX, and the ...

Embodiment 2

[0039] Propagate Almond Vernonia by following these steps:

[0040] (1) Induction of explants: Stems were cut from Vernonia amygdala plants, which were growing robustly and free from diseases and insect pests, as explants, soaked in a diluted solution of detergent, and then cleaned the surface of the explants with a brush. Then rinse with running water for 0.5h, soak in alcohol with a volume ratio of 75% for 20s on the ultra-clean workbench, rinse with sterile water twice, then disinfect with 1g / L mercury chloride solution for 10min, rinse with sterile water twice Afterwards, inoculate in the MS starting medium containing 0.5mg / L6-BA and 0.05mg / LNAA, the culture temperature is 23°C, the light intensity is 2000LX, and the light time is 10h per day;

[0041] (2) Subculture of induced buds: when the induced buds grow to 2~3cm, inoculate them into MS proliferation medium containing 0.5mg / L6-BA and 0.05mg / LNAA, the temperature is 23°C, and the light intensity is 2000LX. The light ...

Embodiment 3

[0047] Propagate Almond Vernonia by following these steps:

[0048] (1) Induction of explants: Stems were cut from Vernonia amygdala plants, which were growing robustly and free from diseases and insect pests, as explants, soaked in a diluted solution of detergent, and then cleaned the surface of the explants with a brush. Then rinse with running water for 0.8h, soak in alcohol with a volume ratio of 75% for 30s on the ultra-clean workbench, rinse with sterile water for 4 times, then disinfect with 1g / L mercury chloride solution for 8min, and rinse with sterile water for 4 times Afterwards, inoculate in the MS starting medium containing 1mg / L6-BA and 0.05mg / LNAA, the culture temperature is 28°C, the light intensity is 1500~2000LX, and the light time is 15h per day;

[0049] (2) Subculture of induced buds: when the induced buds grow to 2~3cm, they are inoculated into MS proliferation medium containing 0.5mg / L6-BA and 0.1mg / LNAA, the temperature is 28℃, and the light intensity i...

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PUM

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Abstract

The invention discloses a propagation method of vernonia amygdalina. The propagation method is characterized in that a proliferation bud of the vernonia amygdalina is pre-cultured in a rooting culture medium to obtain an aseptic seedling, then the aseptic seedling is cut to be cultured in a matrix, and after the aseptic seedling is rooted and has new leaves, the aseptic seedling is then transplanted into a flowerpot. The vernonia amygdalina is propagated by combining a tissue culture technology and the cutting technology, so that complementary advantages can be realized, and the pre-cultured and non-rooted aseptic seedling is cut into a mixed matrix of peat soil, river sand and vermiculite to be rooted, and the difficulty that the culture medium is washed, so that the seedling is likely to infect harmful bacteria can be overcome, and the survival rate is increased; a great amount of cutting propagation materials can be provided all year round by adopting the tissue culture technology, and the mass production and all-year production requirements of seedlings can be met.

Description

technical field [0001] The invention relates to a propagation method of Vernonia amygdala, in particular to a rapid propagation method of Vernonia amygdala in combination with tissue culture and cutting techniques. Background technique [0002] Vernonia amygdalina Del., also known as Vernonia amygdalina Del., also known as Vernonia amygdalina, Apricot Vernonia, Miracle Tree, Bitter Tree, South Africa Tree, South Africa Leaf, Pintail Phoenix, etc., is a plant of the Asteraceae Vernonia, native to Africa, Most common in West Africa. At present, there are about 1,000 species of Vernonia plants in the Asteraceae family, most of which belong to herbs, woody vines or trees, and mainly grow in tropical regions. About 30 species have been found in my country, including Vernonia rugosa, Vernonia willow, Vernonia repellent, and Nocturnal oxen, etc. The main species are distributed in southwest to southeast, Taiwan, Xinjiang and other places. Vernonia amygdala is mostly a perennial t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 沈彦会周桂英许建新刘文竹唐彪赵克奇刘璞张静王成聪
Owner SHENZHEN TECHAND ECOLOGY & ENVIRONMENT CO LTD
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