Composite scaffold for improving gaps and pores to promote cell adhesion rate and preparation method

Abstract

The invention belongs to the technical field of composite scaffolds and discloses a composite scaffold for improving gaps and pores to promote the cell adhesion rate and a preparation method. A channel pore and a spherical pore of the composite scaffold are 150mum-650mum and 3mum-15mum respectively; the preparation method comprises the following steps: obtaining a CS/HA (chitosan/hydroxyapatite) scaffold with pores and spherical pores by adopting an in situ hybridization technology and a freeze-drying method; connecting CS and RGD (arginine-glycin-aspartate) peptides with hydrogen bonds, and modifying the RGD (arginine-glycin-aspartate) peptides on the surface of a porous channel of the CS/HA scaffold with a static self-assembly method, so as to prepare a three-dimensional porous CS/HA scaffold with efficient adherent cells containing the RGD peptides. The scaffold has better cytocompatibility; through composition with RGD, the functions of attraction, induction and growth control of the three-dimensional porous CS/HA scaffold on cells are obviously enhanced; a physical absorption method and a chemical fixation method are combined in the preparation method, so that the material hasbetter adsorbability and stability.

Description

technical field [0001] The invention belongs to the technical field of composite scaffolds, and in particular relates to a composite scaffold and a preparation method for improving the gap and pores to promote cell adhesion rate. Background technique [0002] Bone defect is a common clinical problem in orthopedics. Every year, as many as 2.2 million people in the world need bone transplantation due to trauma, infection, tumor and other reasons. In the United States, about 60% of patients who need bone grafting use autologous bone every year. For transplantation, 34% used allogeneic bone grafts, and only 7% of patients used other bone graft materials. For nonunions and bone defects caused by various bone diseases such as trauma and bone tumors, autologous or allogeneic bone grafts are usually used for treatment. Although these methods are also very effective, they still often encounter insufficient material sources, the risk of infection of donor diseases, and difficulties i...

AI Technical Summary

Problems solved by technology

[0015] To sum up, the problems existing in the prior art are: most of the current research on bone tissue engineering scaffolds requires long-term culture of cells and scaffolds in vitro, which not only prolongs the treatment time, but also increases the cost of treatment and increases the suffering of patients.

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