A kind of colonization probiotic preparation and its application and medicine

A technology of probiotic preparations and probiotics, which is applied in the field of colonization probiotic preparations and their applications and medicines, can solve the problems of difficulty in fully exerting the functions of probiotics and difficulty in adhering the adhesion rate, etc., to achieve increased adhesion rate, high adhesion rate, and improved Effects of Probability and Speed

Active Publication Date: 2018-07-31
邵萍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the existing probiotic preparations that act on the respiratory mucosa can be taken away from the respiratory tract by the mucus blanket driven by cilia in just over 10 minutes under the transport of respiratory mucus. Therefore, the existing probiotic preparations that directly act on the respiratory mucosa have difficulties Adhesion, that is, the problem of low adhesion rate, which makes it difficult for the corresponding probiotics to fully exert their functions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] The colonization probiotic preparation provided in this embodiment is used to directly act on the mucosa of the respiratory tract and contains a surfactant and a probiotic. Among them, the probiotic is Lactobacillus salivarius, the surfactant is lichenysin, and the concentration of Lactobacillus salivarius is 1x10 8 CFU / ml, lichenin content is 5mg / L, surface tension is 55mN / m, NaCl content is 9g / kg, pH is 5.5. The colonization probiotic preparation is an aqueous suspension.

[0077] 1.1 The preparation method of the colonization probiotic preparation provided in this example:

[0078] 1.1.1 Select Lactobacillus salivarius (Lactobacillus salivarius, ATCC 11741) for cultivation. Cultivation conditions: 28°C, inoculation amount of 5%, MRS medium, anaerobic culture, initial pH of MRS medium 5.5, culture for 15 hours.

[0079] 1.1.2 After the culture is over, centrifuge at 4500 rpm for 10 minutes to collect Lactobacillus salivarius bacteria.

[0080] 1.1.3 Select Bacillus lichenif...

Embodiment 2

[0114] The colonization probiotic preparation provided in this embodiment is used to directly act on the mucosa of the respiratory tract and contains a surfactant and a probiotic. Among them, the probiotic is Lactobacillus casei, the surfactant is surfactin, the surface tension of the colonized probiotic preparation is 55mN / m, the pH is 6, the NaCl content is 9g / L, and the surfactant is surfactin. The content is 14.5mg / L, and the probiotic content is 1×10 8 CFU / ml.

[0115] 2.1 The preparation method of the colonization probiotic preparation provided in this example is as follows:

[0116] 2.1.1 Select Lactobacillus casei (Lactobacillus casei, ATCC 393). Inoculate the overnight cultured fresh bacterial solution, inoculate to MRS medium, the inoculum amount is 1%, the culture temperature is 28℃, and the anaerobic culture is allowed to stand in the incubator for about 15 hours. After the cultivation, the cells were centrifuged at 4500 rpm for 10 minutes to collect Lactobacillus cas...

Embodiment 3

[0128] The colonization probiotic preparation provided in this embodiment is used to directly act on the mucosa of the respiratory tract and contains a surfactant and a probiotic. Among them, the probiotic is inactivated Lactobacillus casei, the surfactant is surfactant, the surface tension of the colonization probiotic preparation is 40mN / m, the pH is 5.5, the NaCl content is 9g / L, and the surfactant content is 16mg / L, the content of inactivated probiotics is 1×10 8 Pcs / ml.

[0129] 3.1 The preparation method of the colonization probiotic preparation provided in this example:

[0130] The preparation method of the colonization probiotic preparation provided in this example is basically the same as the step 2.1 in Example 2, except that after the Lactobacillus casei cells are collected in this example, they are heat-inactivated at 80°C for 20 minutes, and then the inactivated Lactobacillus casei cells and sterile culture medium supernatant are made into colonization probiotic pre...

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Abstract

A colonizing probiotic preparation directly acting on the respiratory mucosa, comprising a surfactant and probiotics. The surfactant in the preparation not only increases the probability of and the rate at which the probiotics come into contact with respiratory mucosal cells, but also increases the adhesion rate of the probiotics on respiratory mucosal epithelial cells. The preparation is applicable in preparing a medicament for treating rhinitis, asthma, atopic dermatitis, cranial nerve disease, nasopharyngeal cancer, lung cancer, cold, or respiratory infection.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular, to a planting probiotic preparation and its application and medicine. Background technique [0002] The respiratory tracts of terrestrial vertebrates such as humans, livestock, and poultry have long-term circulation of air containing microorganisms. The mucous membranes distributed on the inner surface of the respiratory tract constantly filter microorganisms in the air. Therefore, a wide variety of microorganisms inevitably exist in the respiratory tract of terrestrial animals. These microorganisms influence each other and also interact with the mucous membrane of the respiratory tract to form a sustainable micro-ecology. [0003] Due to the connectivity of the respiratory tract, the microbial populations distributed on the respiratory tract of the same individual are basically the same. However, due to the effect of the Mucociliary Transport System (MCT) of the respiratory tract,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/744A61K35/747A61K9/00A61K47/28A61K47/42A61K47/26A61P35/00A61P25/00A61P11/00A61P11/02A61P11/06A61P17/00A61P37/08
CPCA61K9/0082A61K35/744A61K35/747A61K47/26A61K47/28A61K47/42
Inventor 杜斌
Owner 邵萍
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