Lotus seed resistant starch with high bifidobacterium breve adhesion rate and preparation method and application thereof

A bifidobacterium breve and resistant starch technology, applied to lotus seed resistant starch with high bifidobacterium breve adhesion rate and its preparation and application, can solve the problems of different adhesion ability, achieve improved effect and enhance resistance , the effect of prolonging the viability

Active Publication Date: 2021-06-25
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Different Bifidobacterium breve strains have different adhesion abilities to starch granules. There is no report on the adhesion ability of Bifidobacterium breve to starch granules, nor is there any report on resistant starch that can improve the adhesion rate of Bifidobacterium breve. to report

Method used

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  • Lotus seed resistant starch with high bifidobacterium breve adhesion rate and preparation method and application thereof
  • Lotus seed resistant starch with high bifidobacterium breve adhesion rate and preparation method and application thereof
  • Lotus seed resistant starch with high bifidobacterium breve adhesion rate and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A method for preparing lotus seed resistant starch MP-LRS3-1 with a high adhesion rate of Bifidobacterium breve, comprising the following process steps:

[0045] (1) Extraction of lotus seed starch: add water to fresh lotus and crush it in a juice extractor, wherein the weight ratio of fresh lotus to water is 1:2; after that, pass through a 120 mesh sieve, let the filtrate stand for 6h to settle, and then discard Remove the supernatant; wash the lower layer with industrial alcohol for 3 times until the supernatant has no yellow oil, discard the supernatant; wash the lower layer with distilled water, and then let it stand for 4 hours, discard the supernatant, and place it in a 45°C oven , baked for 4 hours, so that the moisture content was 11.7%, to obtain lotus seed starch;

[0046](2) Preparation of starch milk: the lotus seed starch prepared in step (1) is placed in a beaker, and distilled water is added to prepare starch milk with a concentration of 15%;

[0047] (3...

Embodiment 2

[0052] A method for preparing lotus seed resistant starch MP-LRS3-2 with a high adhesion rate of Bifidobacterium breve, comprising the following process steps:

[0053] (1) Extraction of lotus seed starch: add water to fresh lotus and crush it in a juice extractor, wherein the weight ratio of fresh lotus to water is 1:2; after that, pass through a 120 mesh sieve, let the filtrate stand for 6h to settle, and then discard Remove the supernatant; wash the lower layer with industrial alcohol for 3 times until the supernatant has no yellow oil, discard the supernatant; wash the lower layer with distilled water, and then let it stand for 4 hours, discard the supernatant, and place it in a 45°C oven , baked for 4 hours, so that the moisture content was 11.7%, to obtain lotus seed starch;

[0054] (2) Preparation of starch milk: the lotus seed starch prepared in step (1) is placed in a beaker, and distilled water is added to prepare starch milk with a concentration of 15%;

[0055] (...

Embodiment 3

[0060] A method for preparing lotus seed resistant starch WB-LRS3-1, which includes the following process steps:

[0061] (1) Extraction of lotus seed starch: add water to fresh lotus and crush it in a juice extractor, wherein the weight ratio of fresh lotus to water is 1:2; after that, pass through a 120 mesh sieve, let the filtrate stand for 6h to settle, and then discard Remove the supernatant; wash the lower layer with industrial alcohol for 3 times until the supernatant has no yellow oil, discard the supernatant; wash the lower layer with distilled water, and then let it stand for 4 hours, discard the supernatant, and place it in a 45°C oven , baked for 4 hours, so that the moisture content is 11.7%, that is, lotus seed starch;

[0062] (2) Preparation of starch milk: the lotus seed starch prepared in step (1) is placed in a beaker, and distilled water is added to prepare starch milk with a concentration of 30%;

[0063] (3) Water-bath gelatinization of starch: the starc...

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Abstract

The invention relates to lotus seed resistant starch with a high bifidobacterium breve adhesion rate as well as a preparation method and application thereof. The preparation method of the lotus seed resistant starch comprises the following steps: extraction of lotus seed starch, preparation of starch milk, gelatinization of starch, preparation of crude lotus seed resistant starch and purification. The lotus seed resistant starch has a high bifidobacterium breve adhesion rate, can be applied to adhesion of bifidobacterium breve, and further can be applied to preparation of bifidobacterium breve preparations or products, so that the effectiveness of bifidobacterium breve related products is improved, the resistance of bifidobacterium breve to a low pH value is enhanced, the viability of bifidobacterium breve in food is prolonged, and meanwhile, the effect of the bifidobacterium breve related food entering the human body is improved.

Description

technical field [0001] The invention relates to a lotus seed resistant starch with high adhesion rate of Bifidobacterium breve and its preparation method and application. Background technique [0002] Lotus seed is a special food with rich nutritional value and "medicine and food from the same source", which is widely used in food processing and industrial production. The starch content in lotus seed starch accounts for more than 50% of the dry matter content, which contains more than 42% of amylose, and the main chain of lotus seed starch is long, the branch chain is short, and the degree of branching is small. It is extremely easy to form lotus seeds after gelatinization and aging. resistant starch. Resistant starch, also known as enzyme-resistant starch, is defined as "the sum of starch that cannot be absorbed and degraded in the small intestine", and has physiological functions such as regulating intestinal metabolism, regulating blood sugar and blood lipids, and reduci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B30/04
CPCC08B30/04Y02A50/30
Inventor 邓凯波黄雅萍杨书捷薛旭亮刘霞郑宝东张怡卢旭
Owner FUJIAN AGRI & FORESTRY UNIV
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