60 results about "Osteoblastic cell" patented technology

The invention discloses a recombinant human-derived II-type collagen, a nucleic acid molecule, a carrier, a cell, a composition and application thereof, and relates to the technical fields of genetic engineering and synthetic biology. The recombinant human-derived II-type collagen comprises a calcium ion binding domain and has an effect of promoting osteoblast proliferation, so that the recombinant human-derived II-type collagen has a good application prospect in preparation of medical instruments and compositions. The recombinant human II-type collagen provided by the invention can be expressed in a common expression system, and has the advantages of high expression quantity and no endotoxin hidden danger. Moreover, the recombinant human II-type collagen does not carry purification labels such as histidine labels and the like, and can be purified through a molecular sieve, so that the target protein can be directly obtained finally, and the purification labels such as the histidine labels and the like do not need to be additionally cut off. The production method of the recombinant human-derived II-type collagen is suitable for industrial large-scale production, and the prepared product does not have an animal-derived infection source, so that the biological safety is higher.

The invention relates to a collagen scaffold with a fixed-axis orientation structure and a preparation method and application thereof, and the preparation method comprises the following steps: (1) dropwise adding a soluble phosphate solution into a collagen-soluble calcium salt mixed solution, and adjusting the pH value with ammonia water; (2) injecting the obtained mixed solution into a mold of which the bottom is provided with a heat-conducting bottom plate; vacuumizing is conducted, heat insulation treatment is conducted on the part, except for the heat conduction bottom plate, of the mold, and then the mold is frozen and formed at the temperature ranging from-20 DEG C to-180 DEG C; and (3) freeze drying. According to the preparation method, heat insulation treatment is conducted on the part, except for a heat conduction bottom plate, of a mold, only the heat conduction bottom plate is left for heat exchange with the external environment, during freezing, low temperature is gradually transmitted upwards from the heat conduction bottom plate to form a temperature gradient, ice crystals of an oriented structure are formed, and then the ice crystals are removed through freeze drying. The interior of the scaffold can be provided with a structure which is similar to natural bones and is arranged in a Harvard system, so that osteoblast proliferation is facilitated, and good conditions are created for growth of cells and blood vessels.

The invention discloses a multifunctional coating with a compact-porous double-layer structure on the surface of magnesium alloy and a preparation method, and belongs to the technical field of biological materials. According to the multifunctional coating, magnesium alloy serves as a substrate, a compact layer and a porous layer are sequentially stacked from the substrate to the outside, and the compact layer is composed of two-dimensional transition metal carbide / nitride, calcium silicate nanowires and a positively charged polymer. The porous layer is composed of copper-containing complex / biopolymer composite fibers, mineralized silk fibers loaded with bioactive metal ions and astragalus polysaccharide on the surface, and natural polymers. According to the multifunctional coating with the compact-porous double-layer structure prepared on the surface of the magnesium alloy, the porous layer on the surface layer has a unique porous structure, growth and proliferation of osteoblasts can be well promoted, meanwhile, the multifunctional coating has the characteristics of pressure resistance, bacteria resistance and oxidation resistance, the compact layer on the bottom layer can improve the corrosion resistance of the magnesium alloy, and the service life of the magnesium alloy is prolonged. The coating is particularly suitable for surface coating of magnesium alloy implant materials and can be used in the field of medical instruments.

The invention provides modified milk powder capable of promoting bone health as well as a preparation method and application of the modified milk powder. The total protein content of the modified milk powder is 17-35 g / 100 g, the fat content of the modified milk powder is 1-32 g / 100 g, and the calcium content of the modified milk powder is 0.8-2.1 g / 100 g. Moreover, the modified milk powder contains bovine coloctrum and lactoferrin, the mass ratio of the bovine coloctrum to the lactoferrin is (5-100): 1 on the basis of the dry matter content, and the content of the lactoferrin in the modified milk powder is 2-400 mg / 100 g. The bovine colostrum and the lactoferrin are combined for preparing the milk powder, have a synergistic effect on promoting osteoblasts and inhibiting osteoclast proliferation, and are beneficial to bone health.

The invention discloses a novel collagen functional variant amino acid and functional application thereof, and relates to the technical field of collagen variants, the amino acid of the collagen functional variant is a COL fragment which is extracted from full-length human COL1 and has the length of 30 amino acids, and a collagen variant C109 synthesized by polypeptide has the structural formula shown in the description. It is verified that the variant has a self-assembly structural property and a good biological activity function, and the amino acid sequence of the collagen functional variant is GLPGPSGEPGKQGPSGASGERGPPGPMGPP (SEQ ID No. 1); the polypeptide formed by the collagen functional variant provided by the invention has the same self-assembly capability as full-length collagen, has better biological activity, is non-toxic, can promote osteoblast differentiation, and supports tissue regeneration at the same time.

The invention relates to an antibacterial and antioxidant Ti-MXene bone implant material triggered by near-infrared light and a preparation method of the Ti-MXene bone implant material. The material is composed of a pure Ti substrate and a plurality of Ti3C2TX MXene coatings which are electrostatically self-adsorbed on the pure Ti substrate, and then is crosslinked and coated by gelatin. The multi-layer Ti3C2TX MXene is combined with the surface of a metal matrix, and has excellent biocompatibility and antibacterial and antioxidant effects. According to the coating, multiple layers of Ti < 3 > C < 2 > TX MXene are stripped from an MAX phase (Ti < 3 > Al < 2 >) through hydrochloric acid etching, then a Ti sheet is soaked in alkali liquor with dispersed MXene, and titanate and MXene are subjected to electrostatic self-bonding on the surface; and the combination is further enhanced through gelatin coating. The material can realize photo-thermal antibacterial ability under the irradiation of 808nm NIR with lower power, and has good antioxidant ability and osteoblast compatibility.

The present invention is directed to obtaining a cell population comprising mesenchymal stem cells that are excellent in proliferative capability and osteoblastic differentiation capability and are useful for the regeneration of periodontal tissues. The present invention is also directed to providing a cell sheet and a method for producing a cell sheet, comprising, as a material, a cell population comprising mesenchymal stem cells that are excellent in proliferative capability and osteoblastic differentiation capability and are useful for the regeneration of periodontal tissues. A cell population having alkaline phosphatase activity before calcification induction of 1 U or more is selected and obtained from a cell population comprising mesenchymal stem cells derived from a dental or periodontal tissue. A cell sheet is obtained by the following steps (a) to (c): (a) obtaining a cell population comprising mesenchymal stem cells and having alkaline phosphatase activity; (b) culturing the cell population in a calcification induction medium, and proliferating the cell population on a plastic substrate; and (c) recovering and washing a sheet-shaped cell population formed on the plastic substrate.

A high-strength porous metal implant material comprises a three-dimensional interconnected porous structure with a porosity of 55-90%, and two or more reinforcing elements distributed at the edge of the porous structure. The reinforcing elements are integrally formed and seamlessly bonded to each other and to the porous material. The reinforcing element structure of the porous metal implant material improves the material's mechanical strength. Its porous structure promotes bone tissue ingrowth into the porous metal's pores, facilitates osteoblast adhesion, differentiation, and growth, enhances the integration of the implant with bone, and achieves long-term biological fixation of the implant to human tissue. The thickness of the reinforcing elements, their positional distribution, or their volume percentage within the overall material can be adjusted according to the stress or mechanical performance requirements of the bone repair or bone defect site, thereby enabling the production of bone filling and implant materials that meet the different mechanical strength requirements of various locations.

The invention relates to a biphase calcium phosphate / hydroxyapatite bone substitute material and a preparation method thereof. The bone substitute material comprises a degradable calcium phosphate core layer, a porous hydroxyapatite precursor layer and a hydroxyapatite gradient layer, wherein the porous hydroxyapatite precursor layer is formed on the surface of the degradable calcium phosphate core layer in situ through a sol-gel method, and the hydroxyapatite gradient layer is formed through electro-deposition treatment and covers the porous hydroxyapatite precursor layer. According to the technical scheme, a layered mineralization structure with a porous hydroxyapatite precursor layer as a template is constructed, and a hydroxyapatite layer with a continuous gradient in the thickness direction is formed through segmented electro-deposition treatment, so that the obtained composite material realizes continuous transition from outside to inside in the aspects of porosity, grain size, crystallinity and Ca / P ratio. The structure of the biphase calcium phosphate / hydroxyapatite bone substitute material can remarkably improve the interface bonding strength, the ion exchange capacity and the biological activity, so that the osteoblast adhesion, proliferation and osteogenic differentiation capacities are improved, and the overall osteogenic performance is obviously superior to that of an existing material.

The invention discloses an electrostatic spinning nanofiber membrane for promoting jawbone defect osteogenesis and a preparation method, relates to the technical field of medical tissue engineering materials, and aims to solve the problem that a tissue engineering scaffold applied to periodontal tissue regeneration is mostly soaked in a solution, and the physical loading mode is not beneficial to long-term stable release of growth factors. In the prior art, metal ions are often used for activating osteoblasts, but the activation effect is limited, so that the metal ions are difficult to promote the regeneration of periodontal tissues because the metal ions are difficult to fully mobilize the initiative of the cells. The invention provides an electrostatic spinning nanofiber membrane for promoting jaw defect osteogenesis and a preparation method thereof. The preparation method comprises the following steps: preparing freeze-dried CGF powder; preparing a PVA / SA / nHA solution, and adding the freeze-dried CGF powder to obtain a spinning solution; and collecting the spinning solution on an aluminum foil roller to obtain the PVA / SA / CGF / nHA electrostatic spinning nanofiber membrane. Concentrated growth factors can be continuously and stably released, and osteogenic differentiation of peripheral jawbones is promoted.

The invention relates to the field of titanium and titanium alloy processing, in particular to a laser-assisted prepared novel anti-stripping TNTs dental implant, and a preparation method of a novel anti-stripping L-TNTs array film comprises the following steps: (1) spin-coating photoresist on a non-pattern area on the surface of a titanium-based material; (2) etching a pattern area on the surface of the titanium-based material; (3) carrying out anodic oxidation on the etched region; and (4) removing the photoresist to obtain the novel anti-stripping TNTs array film prepared by laser assistance. The surface structure of the novel anti-stripping L-TNTs array film is that TNTs are embedded in a groove formed by etching. According to the novel anti-stripping L-TNTs array film disclosed by the invention, the anti-stripping and wear-resisting properties of the L-TNTs are enhanced through accurate control of the size of the micro-pattern structure of the novel anti-stripping L-TNTs array film; the L-TNTs can also be prepared on the bent surface of the titanium implant, and the capability of promoting osteoblast growth and differentiation of the nanotube structure is still reserved; the L-TNTs improve the performance of the whole implant and promote the commercial application of the TNTs.

The present invention discloses a type X collagen peptide for improving bone growth and its preparation method and application, relating to the field of biotechnology. The preparation method comprises the following steps: S1, eggshell membrane powder is extracted with water to obtain a crude extract; S2, enzyme is added to the crude extract for enzymolysis to obtain an enzymolysis solution; S3, natto Bacillus powder is inoculated in the enzymolysis solution, the inoculation amount is 0.05-1%, the temperature is 38-45 ° C, and the fermentation is 14-36h; the temperature is lowered to 25-30 ° C, lactic acid Kluyveromyces is inoculated, the inoculation amount is 1-6%, and the fermentation is 3-7 days to obtain eggshell membrane enzymatic fermentation liquid; S4, the eggshell membrane enzymatic fermentation liquid is centrifuged, a clarifier is added to obtain a clarified liquid; S5, a decolorizing agent is added to the clarified liquid, and it is dried to obtain a type X collagen peptide. The collagen peptide of the present invention has a high content of type X collagen, which can promote osteoblast growth and has broad application prospects in the preparation of growth-promoting products.

The invention provides a mesoporous silica / titanium-based composite material and a preparation method thereof, and belongs to the technical field of medical titanium materials. According to the method, femtosecond laser scanning is conducted on the titanium-based material in the mesoporous silicon dioxide suspension, mesoporous silicon dioxide is deposited on the surface of the titanium-based material in situ in the process that the titanium-based material is ablated through femtosecond laser, and the binding force of the mesoporous silicon dioxide and the titanium-based material is improved; a mesoporous silica micro-nano structure is constructed on the surface of the titanium-based material by using femtosecond laser, so that cell adsorption is facilitated, osteoblasts are promoted to grow on the surface of the material, and the biocompatibility of the material is improved; by controlling the concentration of the mesoporous silica suspension, the deposition amount of mesoporous silica can be increased, and the drug loading capacity is improved; by limiting the parameters of the femtosecond laser, a regular micro-nano structure beneficial to cell adsorption can be constructed.

The application relates to the technical field of dental implants, in particular to a trans-zygomatic implant and a processing technology thereof, and the processing technology comprises variable-distance multi-stage sand blasting and acid etching surface treatment on an anchoring part with threads of the trans-zygomatic implant and electrochemical polishing surface treatment on an extension part without threads. The trans-zygomatic implant is treated by variable-distance multi-stage sand blasting combined with acid etching, so that a uniform micron-level rough base and a multi-stage micropore structure can be formed on the titanium surface, osteoblast adhesion and differentiation are facilitated, the early bone combination speed is accelerated, the combination strength is improved, the early implant loosening risk is reduced, the extension part of the trans-zygomatic implant is treated by electrochemical polishing, a smooth surface can be obtained, plaque adhesion and bacterial colonization are greatly reduced, the implant peri-implantitis risk is reduced, and soft tissue adhesion is promoted; the dual optimization of bone anchoring and tissue adhesion is realized, and the long-term function and health of the trans-zygomatic implant under complex stress are guaranteed.

The invention discloses a preparation method of a GelMA / ZIF-8 / MEL hydrogel coating on the surface of a porous titanium alloy, and belongs to the field of surface modification of a biomedical material porous titanium alloy implant. A ZIF-8 loaded MEL system is constructed, the system is loaded to the surface of a titanium alloy prosthesis through GelMA, and the biological function that the porous titanium alloy implant regulates and controls the oxidative stress environment of the prosthesis and a bone interface is achieved. According to the method, the GelMA / ZIF-8 / MEL hydrogel coating is applied to surface modification of the porous titanium alloy implant for the first time, and an implant interface with biological activity is constructed; mEL is loaded on ZIF-8, and GelMA is applied to the surface of the porous titanium alloy implant, so that the half-life period of a drug is prolonged, the local drug concentration is reduced, the side effect is relieved, a synergistic osteogenic differentiation effect is generated on osteoblasts, and the biological activity of the porous titanium alloy implant is improved; the preparation of the GelMA / ZIF-8 / MEL hydrogel coating is not limited by the shape of the matrix, and the bioactive coating can be prepared on the matrix with a complex shape.

The application discloses a prosthesis capable of fixing a proximal femur in hip joint revision surgery and relates to the technical field of medical devices.The prosthesis comprises a femoral stem body and a dynamic auxiliary structure.The dynamic auxiliary structure comprises a side groove, a counterweight and a limiting seat.The side groove is provided through the wide side of the femoral stem body.The two side ports of the side groove are provided with elastic plates, and the side groove is provided with a guide rod.The counterweight is movably sleeved on the guide rod.A traction spring is arranged between the top of the counterweight and the end of the side groove.The two sides of the counterweight are embedded with protrusions.The limiting seat is arranged on the inner wall of the side groove and located on the two sides of the counterweight.A guide column is slidably arranged through the limiting seat, and the end of the guide column close to the counterweight is provided with a transmission seat corresponding to the protrusions.The prosthesis can reduce the micromotion of the prosthesis and the femoral medullary cavity, promote bone ingrowth, reduce the incidence of long-term loosening, activate osteoblast activity, inhibit osteoclast proliferation, maintain the bone mass around the prosthesis, and prolong the service life of the prosthesis.

The present invention provides a cartilage repair membrane and a preparation method thereof. The cartilage repair membrane includes an umbilical cord layer, a first gel layer, and a second gel layer that are stacked in sequence. The umbilical cord layer is a sheet-like structure composed of umbilical cord tissue, and the umbilical cord tissue is formed from an umbilical cord from which vascular cells have been removed by mechanical means. Both the first gel layer and the second gel layer are formed by mixing and cross-linking chitosan, sodium alginate, and hydroxyapatite, and the concentration of hydroxyapatite in the first gel layer and the second gel layer is different. The cartilage repair membrane of the present invention has good biological activity, mechanical properties, porosity, and osteogenic properties, provides the best repair effect for the treatment of cartilage defects, and has significant effects in aspects such as antibacterial, hemostatic, promoting the adhesion, proliferation of osteoblasts, and the formation of mineralized bone matrix, can meet the requirements of clinical and tissue engineering to the greatest extent, and can improve the repair degree of articular cartilage injury.

The invention belongs to the field of electrochemistry, and relates to a micro-arc oxidation method for a tantalum metal surface in a sodium chloride electrolyte. According to the micro-arc oxidation method for the tantalum metal surface, tantalum metal serves as an anode, micro-arc oxidation treatment is carried out, and a Ta2O5 coating is obtained on the surface; electrolyte used for micro-arc oxidation treatment is sodium chloride. According to the method, the Ta2O5 oxidation film with excellent corrosion resistance is obtained through micro-arc oxidation, the method is simple in preparation condition, the electrolyte is completely green and environmentally friendly, the film forming speed is high, the prepared Ta2O5 passivation film with the porous film layer is obtained, Ta2O5 in the film layer has high corrosion resistance, the porous coating can promote proliferation and adhesion of osteoblasts, and the service life of the film layer is prolonged. The porous Ta2O5 oxide film prepared by the preparation method disclosed by the invention is controllable in structure and morphology, and can be flexibly regulated and controlled according to requirements.

The invention relates to the technical field of dental and orthopedic implants, in particular to a zirconia osseointegration implant material and a preparation method thereof. Aiming at the problems of strong biological inertness and slow early-stage osseointegration of the zirconium oxide ceramic surface, the preparation method comprises the following steps: cleaning a zirconium oxide matrix and carrying out alkaline activation; forming a polydopamine adhesion bottom layer; branched polyethyleneimine is fixed to enhance the amino density; constructing a network layer through alternate coordination of phytic acid and calcium / strontium ions; strontium doped hydroxyapatite nano particles are immobilized; finally, biomimetic mineralization is carried out in simulated body fluid. According to the method, a micro-nano composite active interface is formed, the biological activity, ion release controllability and osseointegration capability of the material are remarkably improved, and osteoblast response is promoted.

The invention belongs to the field of biological medicine, and particularly relates to application of a reagent for knocking down or inhibiting ANT1 to preparation of a medicine for preventing and / or treating heterotopic ossification. The application of the reagent for knocking down or inhibiting ANT1 in preparation of the medicine for preventing and / or treating heterotopic ossification is found for the first time. After ANT1 is inhibited, heterotopic ossification formed after soft tissue injury can be remarkably reduced; infiltration of osteoblasts, chondrocytes and inflammatory cells in injured tendons is reduced, formation of marrow cavities and blood vessels is reduced, and formation of bone tissues is reduced; the expression of osteogenesis related markers OCN, RUNX2 and ALP in the injured tendon is obviously inhibited; and ectopic ossification can be effectively prevented and / or treated.

A scaffold comprising an aligned fiber. Further, a scaffold comprising one or more electrospun fibers wherein a fast Fourier transform (FFT) analysis result of the fibers have adjacent major peaks with about 180° apart from each other. Also, methods for promoting differentiation of stem cells into osteoblasts, chondrocytes, ligament or tendon, the method comprising culturing the cells on the scaffold or aligned fiber in conditions suitable for the cell differentiation.

In some aspects, the disclosure relates to compositions and methods for modulating (e.g., increasing and / or decreasing) bone mass in a subject. In some aspects, the disclosure provides isolated nucleic acids, and vectors such as rAAV vectors, configured to express transgenes that promote (e.g., increase) or inhibit (e.g., decrease) activity, differentiation, or function of certain types of bone cells, for example osteoblasts, osteoclasts, osteocytes, etc. In some embodiments, the isolated nucleic acids and vectors described by the disclosure are useful for treating disorders and conditions associated with increased bone mass (e.g., osteopetrosis) or decreased bone mass (e.g., osteoporosis).

The present application relates to a kind of preparation method of titanium dental implant surface coating with antibacterial and bone formation performance, belong to dental implant material technical field.The method is through titanium substrate pretreatment, inner layer hydroxyapatite, chitosan composite coating preparation, outer layer zinc-loaded mesoporous silane coating preparation three-step composite process, constructs double-layer function synergic coating on titanium substrate surface.Inner layer utilizes the osteogenic activity of hydroxyapatite and the natural antibacterial property of chitosan to form basic function layer, outer layer is loaded with zinc ion through the slow-release structure of mesoporous silane, realizes the long-acting controllable release of antibacterial component, while zinc ion and mesoporous silane synergistically improve the proliferation and differentiation ability of osteoblasts.The present application solves the technical problems of single function, poor antibacterial durability, insufficient bone formation effect, weak coating and substrate bonding force and complex preparation process of existing titanium dental implant coating.The prepared coating is firmly combined with titanium substrate, has high biological safety, simple and controllable process, is suitable for industrial production, and has important application value in the field of dental implant.

The invention discloses a surface treatment method of a silicon nitride dental implant, which comprises the following steps: mixing ceramic powder with a sintering aid, and sintering at high temperature to obtain a compact silicon nitride ceramic base material; the base material is polished, and a dental implant precursor with the surface roughness Ra lower than 20 nm is obtained; removing a surface glass phase through selective etching of an acid-base compound, and exposing silicon nitride crystal grains; and finally, cleaning and drying to obtain the silicon nitride dental implant. The surface roughness Ra of the obtained silicon nitride dental implant is 40-100 nm, the contact angle is lower than 20 degrees, and the zeta potential absolute value is larger than 30 mV. The dental implant treated by the method disclosed by the invention can be used for remarkably promoting osteoblast adhesion, proliferation and differentiation while keeping high mechanical properties (three-point bending strength is more than or equal to 800 MPa, Vickers hardness is more than or equal to 1500 HV and fracture toughness is more than or equal to 7.0 MPa.m < 1 / 2 >), and is also suitable for implantation application in the orthopedics department.

Bone necrosis repair stent with osteoblast implantation function, comprising a metal stent (1), wherein extension blocks (3) are rigidly connected to the outside of the metal stent (1), and the metal stents (1) are pivotally connected to telescopic rods (4) via the extension blocks (3), support blocks (6) are arranged on the metal stent (1), wherein damping pivot axes (12) are rigidly connected to the top of the support blocks (6), and the top of the support blocks (6) are rotatably connected to sleeves (7) via the damping pivot axes (12), wherein fluid outlet openings (11) are formed on the surface of the sleeves (7), and the inside of the sleeves (7) is in contact with inner tubes (5), wherein through holes (16) are provided on the outside of the inner tubes (5), and piston rods (9) are movably inserted through one end of the inner tubes (5), wherein at one end of the piston rods (9) a sealing plug (17) is firmly attached,wherein the edge of the sealing plug (17) is in contact with the inner wall of the inner tubes (5), push plates are firmly connected at the other end of the piston rods (9) and limiting components are arranged on the outside of the sleeves (7).

The present invention relates to an implantable device configured to deliver, to an injured bone site, components for revascularisation and bone repair, the device comprising: a first osteoconductive scaffold component adapted to hold and deliver to the injured bone site, growth factors for inducing cellular events that initiate healing; and comprising a second osteoconductive scaffold component adapted to hold and deliver to the injured bone site, viable autologous osteogenic and / or angiogenic cells, and wherein the device also comprises a third scaffold component adapted to promote bone cell proliferation and vascularity, whereby the scaffold components provide a stable mechanical environment for promoting bone cell proliferation and vascularity. The present invention also relates to a method of manufacture of the implantable device.

This application relates to the field of medical implants, specifically to an osteogenic intervertebral fusion device and its preparation method, comprising the following steps: Step 1: 3D printing tantalum metal powder to obtain a base layer; Step 2: 3D printing tantalum metal powder and osteogenic bioceramic onto the base layer to form a transition layer, obtaining a matrix; Step 3: Plasma-spraying osteogenic bioceramic onto the matrix to form a bioceramic layer, obtaining a preliminary product; Step 4: Immersing the preliminary product in a mixed solution of strontium phosphate and β-glycerophosphate magnesium phosphate, drying and curing to obtain the osteogenic intervertebral fusion device. In this application, the base layer can autonomously adjust its porosity, and the transition layer allows for a smooth stress transfer from rigid metal to flexible ceramic, reducing interfacial stress concentration. The bioceramic layer and the transition layer form an interlocking structure, enhancing the interfacial shear strength. Strontium phosphate and β-glycerophosphate magnesium phosphate are enriched on the material surface, which can both rapidly induce osteoblast migration and continuously promote mineralization.

The invention discloses a preparation method of a porous composite material based on hydroxyapatite, magnesium oxide and tannic acid, which comprises the following steps: by taking polyethylene glycol diacrylate as a photo-crosslinking monomer and diphenyl (2, 4, 6-trimethylbenzoyl) phosphine oxide as a photoinitiator, carrying out a hydrothermal reaction to prepare the porous composite material based on hydroxyapatite, magnesium oxide and tannic acid. Nano hydroxyapatite, nano magnesium oxide and tannic acid are uniformly dispersed in a monomer solution to form photocuring slurry, and the photocuring slurry is formed under ultraviolet light through a photocuring 3D printing technology. Wherein the nano hydroxyapatite provides osteoconductivity, the nano magnesium oxide releases magnesium ions to promote osteoblast differentiation, and the tannic acid endows the material with antibacterial and anti-inflammatory properties; according to the composite material bone scaffold, a Gyandroid structure in a three-period extremely small curved surface is adopted as a scaffold structure, and the structure is combined with the functional synergistic effect of all components, so that the prepared composite material bone scaffold has high porosity, excellent compressive strength, remarkable biological activity and active antibacterial and anti-inflammatory capacity at the same time, and repair and regeneration of bone tissues can be effectively promoted.

To provide a novel method of producing an osteoblast cluster, capable of yielding an osteoblast cluster having a high bone regeneration ability through the use of iPS cells as the source material.SOLUTION: Provided is a method of producing an osteoblast cluster from iPS cells, the method including the steps of: (1) inducing formation of an embryoid by subjecting undifferentiated iPS cells to non-adherent culture; (2) inducing differentiation of the iPS cells into mesodermal cells by subjecting the embryoid of the iPS cells obtained in the step (1) to non-adherent culture; and (3) inducing differentiation into osteoblasts by subjecting the mesodermal cells of the iPS cells obtained in the step (2) to non-adherent culture, wherein the steps (1) and (2) are each performed using a culture vessel comprising a bottom surface and a circular side wall standing upright from the bottom surface, the bottom surface having a plurality of depressed portions disposed independently of each other.SELECTED DRAWING: None

The invention relates to the technical field of porous materials, and discloses a method for improving the biocompatibility of a porous implant. The method comprises the following steps: carrying out first ultrasonic treatment on a porous implant made of a stainless steel material; the porous implant subjected to ultrasonic treatment is placed in an anodic oxidation electrolyte containing ammonium fluoride, and anodic oxidation treatment is conducted at the constant temperature and the constant pressure; and carrying out second ultrasonic treatment on the porous implant subjected to anodic oxidation. The porous implant is modified by using a reverse high-voltage anodic oxidation method, and residual surface powder is removed. After anodic oxidation treatment, low-frequency ultrasound is applied to further destroy the structures, so that removal of residual powder in a deep area of the porous implant is promoted, and a new and stable micro-nano surface pattern is generated. The newly generated micro-nano surface pattern is beneficial to the adhesion of osteoblasts, the mineralization process of the cells can be obviously promoted, the interaction between the cells and the implant is promoted, and the biocompatibility of the porous implant is improved.