26 results about "Osteoblastic cell" patented technology

The invention provides modified milk powder capable of promoting bone health as well as a preparation method and application of the modified milk powder. The total protein content of the modified milk powder is 17-35 g / 100 g, the fat content of the modified milk powder is 1-32 g / 100 g, and the calcium content of the modified milk powder is 0.8-2.1 g / 100 g. Moreover, the modified milk powder contains bovine coloctrum and lactoferrin, the mass ratio of the bovine coloctrum to the lactoferrin is (5-100): 1 on the basis of the dry matter content, and the content of the lactoferrin in the modified milk powder is 2-400 mg / 100 g. The bovine colostrum and the lactoferrin are combined for preparing the milk powder, have a synergistic effect on promoting osteoblasts and inhibiting osteoclast proliferation, and are beneficial to bone health.

A high-strength porous metal implant material comprises a three-dimensional interconnected porous structure with a porosity of 55-90%, and two or more reinforcing elements distributed at the edge of the porous structure. The reinforcing elements are integrally formed and seamlessly bonded to each other and to the porous material. The reinforcing element structure of the porous metal implant material improves the material's mechanical strength. Its porous structure promotes bone tissue ingrowth into the porous metal's pores, facilitates osteoblast adhesion, differentiation, and growth, enhances the integration of the implant with bone, and achieves long-term biological fixation of the implant to human tissue. The thickness of the reinforcing elements, their positional distribution, or their volume percentage within the overall material can be adjusted according to the stress or mechanical performance requirements of the bone repair or bone defect site, thereby enabling the production of bone filling and implant materials that meet the different mechanical strength requirements of various locations.

The invention relates to a biphase calcium phosphate / hydroxyapatite bone substitute material and a preparation method thereof. The bone substitute material comprises a degradable calcium phosphate core layer, a porous hydroxyapatite precursor layer and a hydroxyapatite gradient layer, wherein the porous hydroxyapatite precursor layer is formed on the surface of the degradable calcium phosphate core layer in situ through a sol-gel method, and the hydroxyapatite gradient layer is formed through electro-deposition treatment and covers the porous hydroxyapatite precursor layer. According to the technical scheme, a layered mineralization structure with a porous hydroxyapatite precursor layer as a template is constructed, and a hydroxyapatite layer with a continuous gradient in the thickness direction is formed through segmented electro-deposition treatment, so that the obtained composite material realizes continuous transition from outside to inside in the aspects of porosity, grain size, crystallinity and Ca / P ratio. The structure of the biphase calcium phosphate / hydroxyapatite bone substitute material can remarkably improve the interface bonding strength, the ion exchange capacity and the biological activity, so that the osteoblast adhesion, proliferation and osteogenic differentiation capacities are improved, and the overall osteogenic performance is obviously superior to that of an existing material.

The invention discloses an electrostatic spinning nanofiber membrane for promoting jawbone defect osteogenesis and a preparation method, relates to the technical field of medical tissue engineering materials, and aims to solve the problem that a tissue engineering scaffold applied to periodontal tissue regeneration is mostly soaked in a solution, and the physical loading mode is not beneficial to long-term stable release of growth factors. In the prior art, metal ions are often used for activating osteoblasts, but the activation effect is limited, so that the metal ions are difficult to promote the regeneration of periodontal tissues because the metal ions are difficult to fully mobilize the initiative of the cells. The invention provides an electrostatic spinning nanofiber membrane for promoting jaw defect osteogenesis and a preparation method thereof. The preparation method comprises the following steps: preparing freeze-dried CGF powder; preparing a PVA / SA / nHA solution, and adding the freeze-dried CGF powder to obtain a spinning solution; and collecting the spinning solution on an aluminum foil roller to obtain the PVA / SA / CGF / nHA electrostatic spinning nanofiber membrane. Concentrated growth factors can be continuously and stably released, and osteogenic differentiation of peripheral jawbones is promoted.

The invention provides a mesoporous silica / titanium-based composite material and a preparation method thereof, and belongs to the technical field of medical titanium materials. According to the method, femtosecond laser scanning is conducted on the titanium-based material in the mesoporous silicon dioxide suspension, mesoporous silicon dioxide is deposited on the surface of the titanium-based material in situ in the process that the titanium-based material is ablated through femtosecond laser, and the binding force of the mesoporous silicon dioxide and the titanium-based material is improved; a mesoporous silica micro-nano structure is constructed on the surface of the titanium-based material by using femtosecond laser, so that cell adsorption is facilitated, osteoblasts are promoted to grow on the surface of the material, and the biocompatibility of the material is improved; by controlling the concentration of the mesoporous silica suspension, the deposition amount of mesoporous silica can be increased, and the drug loading capacity is improved; by limiting the parameters of the femtosecond laser, a regular micro-nano structure beneficial to cell adsorption can be constructed.

The application relates to the technical field of dental implants, in particular to a trans-zygomatic implant and a processing technology thereof, and the processing technology comprises variable-distance multi-stage sand blasting and acid etching surface treatment on an anchoring part with threads of the trans-zygomatic implant and electrochemical polishing surface treatment on an extension part without threads. The trans-zygomatic implant is treated by variable-distance multi-stage sand blasting combined with acid etching, so that a uniform micron-level rough base and a multi-stage micropore structure can be formed on the titanium surface, osteoblast adhesion and differentiation are facilitated, the early bone combination speed is accelerated, the combination strength is improved, the early implant loosening risk is reduced, the extension part of the trans-zygomatic implant is treated by electrochemical polishing, a smooth surface can be obtained, plaque adhesion and bacterial colonization are greatly reduced, the implant peri-implantitis risk is reduced, and soft tissue adhesion is promoted; the dual optimization of bone anchoring and tissue adhesion is realized, and the long-term function and health of the trans-zygomatic implant under complex stress are guaranteed.

The application discloses a prosthesis capable of fixing a proximal femur in hip joint revision surgery and relates to the technical field of medical devices.The prosthesis comprises a femoral stem body and a dynamic auxiliary structure.The dynamic auxiliary structure comprises a side groove, a counterweight and a limiting seat.The side groove is provided through the wide side of the femoral stem body.The two side ports of the side groove are provided with elastic plates, and the side groove is provided with a guide rod.The counterweight is movably sleeved on the guide rod.A traction spring is arranged between the top of the counterweight and the end of the side groove.The two sides of the counterweight are embedded with protrusions.The limiting seat is arranged on the inner wall of the side groove and located on the two sides of the counterweight.A guide column is slidably arranged through the limiting seat, and the end of the guide column close to the counterweight is provided with a transmission seat corresponding to the protrusions.The prosthesis can reduce the micromotion of the prosthesis and the femoral medullary cavity, promote bone ingrowth, reduce the incidence of long-term loosening, activate osteoblast activity, inhibit osteoclast proliferation, maintain the bone mass around the prosthesis, and prolong the service life of the prosthesis.

The invention relates to the technical field of dental and orthopedic implants, in particular to a zirconia osseointegration implant material and a preparation method thereof. Aiming at the problems of strong biological inertness and slow early-stage osseointegration of the zirconium oxide ceramic surface, the preparation method comprises the following steps: cleaning a zirconium oxide matrix and carrying out alkaline activation; forming a polydopamine adhesion bottom layer; branched polyethyleneimine is fixed to enhance the amino density; constructing a network layer through alternate coordination of phytic acid and calcium / strontium ions; strontium doped hydroxyapatite nano particles are immobilized; finally, biomimetic mineralization is carried out in simulated body fluid. According to the method, a micro-nano composite active interface is formed, the biological activity, ion release controllability and osseointegration capability of the material are remarkably improved, and osteoblast response is promoted.

A scaffold comprising an aligned fiber. Further, a scaffold comprising one or more electrospun fibers wherein a fast Fourier transform (FFT) analysis result of the fibers have adjacent major peaks with about 180° apart from each other. Also, methods for promoting differentiation of stem cells into osteoblasts, chondrocytes, ligament or tendon, the method comprising culturing the cells on the scaffold or aligned fiber in conditions suitable for the cell differentiation.

The present application relates to a kind of preparation method of titanium dental implant surface coating with antibacterial and bone formation performance, belong to dental implant material technical field.The method is through titanium substrate pretreatment, inner layer hydroxyapatite, chitosan composite coating preparation, outer layer zinc-loaded mesoporous silane coating preparation three-step composite process, constructs double-layer function synergic coating on titanium substrate surface.Inner layer utilizes the osteogenic activity of hydroxyapatite and the natural antibacterial property of chitosan to form basic function layer, outer layer is loaded with zinc ion through the slow-release structure of mesoporous silane, realizes the long-acting controllable release of antibacterial component, while zinc ion and mesoporous silane synergistically improve the proliferation and differentiation ability of osteoblasts.The present application solves the technical problems of single function, poor antibacterial durability, insufficient bone formation effect, weak coating and substrate bonding force and complex preparation process of existing titanium dental implant coating.The prepared coating is firmly combined with titanium substrate, has high biological safety, simple and controllable process, is suitable for industrial production, and has important application value in the field of dental implant.

The invention discloses a surface treatment method of a silicon nitride dental implant, which comprises the following steps: mixing ceramic powder with a sintering aid, and sintering at high temperature to obtain a compact silicon nitride ceramic base material; the base material is polished, and a dental implant precursor with the surface roughness Ra lower than 20 nm is obtained; removing a surface glass phase through selective etching of an acid-base compound, and exposing silicon nitride crystal grains; and finally, cleaning and drying to obtain the silicon nitride dental implant. The surface roughness Ra of the obtained silicon nitride dental implant is 40-100 nm, the contact angle is lower than 20 degrees, and the zeta potential absolute value is larger than 30 mV. The dental implant treated by the method disclosed by the invention can be used for remarkably promoting osteoblast adhesion, proliferation and differentiation while keeping high mechanical properties (three-point bending strength is more than or equal to 800 MPa, Vickers hardness is more than or equal to 1500 HV and fracture toughness is more than or equal to 7.0 MPa.m < 1 / 2 >), and is also suitable for implantation application in the orthopedics department.

Bone necrosis repair stent with osteoblast implantation function, comprising a metal stent (1), wherein extension blocks (3) are rigidly connected to the outside of the metal stent (1), and the metal stents (1) are pivotally connected to telescopic rods (4) via the extension blocks (3), support blocks (6) are arranged on the metal stent (1), wherein damping pivot axes (12) are rigidly connected to the top of the support blocks (6), and the top of the support blocks (6) are rotatably connected to sleeves (7) via the damping pivot axes (12), wherein fluid outlet openings (11) are formed on the surface of the sleeves (7), and the inside of the sleeves (7) is in contact with inner tubes (5), wherein through holes (16) are provided on the outside of the inner tubes (5), and piston rods (9) are movably inserted through one end of the inner tubes (5), wherein at one end of the piston rods (9) a sealing plug (17) is firmly attached,wherein the edge of the sealing plug (17) is in contact with the inner wall of the inner tubes (5), push plates are firmly connected at the other end of the piston rods (9) and limiting components are arranged on the outside of the sleeves (7).

The present invention relates to an implantable device configured to deliver, to an injured bone site, components for revascularisation and bone repair, the device comprising: a first osteoconductive scaffold component adapted to hold and deliver to the injured bone site, growth factors for inducing cellular events that initiate healing; and comprising a second osteoconductive scaffold component adapted to hold and deliver to the injured bone site, viable autologous osteogenic and / or angiogenic cells, and wherein the device also comprises a third scaffold component adapted to promote bone cell proliferation and vascularity, whereby the scaffold components provide a stable mechanical environment for promoting bone cell proliferation and vascularity. The present invention also relates to a method of manufacture of the implantable device.

This application relates to the field of medical implants, specifically to an osteogenic intervertebral fusion device and its preparation method, comprising the following steps: Step 1: 3D printing tantalum metal powder to obtain a base layer; Step 2: 3D printing tantalum metal powder and osteogenic bioceramic onto the base layer to form a transition layer, obtaining a matrix; Step 3: Plasma-spraying osteogenic bioceramic onto the matrix to form a bioceramic layer, obtaining a preliminary product; Step 4: Immersing the preliminary product in a mixed solution of strontium phosphate and β-glycerophosphate magnesium phosphate, drying and curing to obtain the osteogenic intervertebral fusion device. In this application, the base layer can autonomously adjust its porosity, and the transition layer allows for a smooth stress transfer from rigid metal to flexible ceramic, reducing interfacial stress concentration. The bioceramic layer and the transition layer form an interlocking structure, enhancing the interfacial shear strength. Strontium phosphate and β-glycerophosphate magnesium phosphate are enriched on the material surface, which can both rapidly induce osteoblast migration and continuously promote mineralization.

The invention discloses a preparation method of a porous composite material based on hydroxyapatite, magnesium oxide and tannic acid, which comprises the following steps: by taking polyethylene glycol diacrylate as a photo-crosslinking monomer and diphenyl (2, 4, 6-trimethylbenzoyl) phosphine oxide as a photoinitiator, carrying out a hydrothermal reaction to prepare the porous composite material based on hydroxyapatite, magnesium oxide and tannic acid. Nano hydroxyapatite, nano magnesium oxide and tannic acid are uniformly dispersed in a monomer solution to form photocuring slurry, and the photocuring slurry is formed under ultraviolet light through a photocuring 3D printing technology. Wherein the nano hydroxyapatite provides osteoconductivity, the nano magnesium oxide releases magnesium ions to promote osteoblast differentiation, and the tannic acid endows the material with antibacterial and anti-inflammatory properties; according to the composite material bone scaffold, a Gyandroid structure in a three-period extremely small curved surface is adopted as a scaffold structure, and the structure is combined with the functional synergistic effect of all components, so that the prepared composite material bone scaffold has high porosity, excellent compressive strength, remarkable biological activity and active antibacterial and anti-inflammatory capacity at the same time, and repair and regeneration of bone tissues can be effectively promoted.

The invention provides a method for preparing a degradable magnesium metal mechanical enhanced piezoelectric composite bone-guided regeneration membrane. A magnesium foil or a magnesium net (which is cut into a magnesium net with small holes through laser cutting) or a magnesium support (which is cut through laser cutting) is firstly subjected to heat treatment by HF (hydrogen fluoride), and a layer of MgF4 coating is obtained on the surface of the magnesium foil or the magnesium net or the magnesium support. Then preparing an electrostatic spinning membrane with a piezoelectric effect on the surface of the sample; preparing a piezoelectric spinning fiber membrane with fibers arranged in parallel on one side close to the fiber tissue so as to shield the ingrowth of the fiber tissue; a disordered and loose piezoelectric spinning fiber membrane is prepared on the side close to the bone tissue by adopting an electrostatic spinning technology. The electrostatic spinning membrane provided by the invention has piezoelectric property, and when the piezoelectric spinning membrane is subjected to physiological mechanical stress (such as muscle contraction and chewing pressure), weak charges can be generated to actively regulate and control physiological activities of osteoblasts and provide repair power for bone regeneration.

A bone screw extending from a distal end portion and a proximal end portion having an optional cannulation is disclosed. The bone screw may include a first threaded section and a second threaded section extending contiguously from the distal end portion toward the proximal end portion defining a dual lead thread pattern. The bone screw may further comprise a third threaded section at distal end portion of the bone screw having a wider thread crest relative to the first and second threaded sections. In some embodiments, the bone screw also comprises a threaded sleeve portion that is configured to receive the shaft of the bone screw. The bone screw also may comprise roughened surface features to provide sufficient osseointegration, cellular attachment, and osteoblast maturation. Methods for manufacturing the bone screw and providing roughened surface features are also disclosed.

A bone collagen scaffold material loaded with multiple growth factors, which includes an acellular matrix for promoting growth of oral osteoblasts and multiple growth factors loaded on the acellular matrix; the acellular matrix is a bone collagen scaffold obtained from natural animal bone containing collagen; the multiple growth factors are FGF, VEGF, TGF-β, and BMP. The bone collagen scaffold material of the invention has a higher collagen content through secondary freeze-drying, making surface of the bone collagen scaffold material have a suitable pore size, guaranteeing mechanical properties and improve bone induction ability, being suitable for loading multiple growth factors and prolonging the degradation time to adapt to the therapeutic effect of periodontitis.

The utility model relates to a turbine-shaped external connection dental implant system, which comprises a dental implant, an external plug-in body, an external connection body, a central screw and a false tooth, the dental implant comprises an implant and a plug-in frame, the implant is used for being implanted in a dental implant cavity, the implant is a turbine-shaped cylinder, the plug-in frame is integrally formed above the implant, and the external connection body is connected with the central screw through the plug-in frame. The plug-in frame is an upper conical cylinder, the outer plug-in body comprises a hollow plug-in body, the hollow plug-in body is a lower conical hollow cylinder, a hollow plug-in part is arranged on the bottom surface of the hollow plug-in body, and the hollow plug-in part is in plug-in locking fit with the plug-in frame; the turbine pieces of the implant are evenly distributed to form a multi-stage bone contact interface, the surface area of the implant and bone tissue is enlarged, three-dimensional climbing of osteoblasts is promoted, the bone regeneration and bone combination process is accelerated, gaps of the turbine pieces and the bone tissue form mechanical interlocking, the overall cylindrical turbine form of the implant is combined, the strong torsional load resistance is provided, and the bone regeneration and bone combination are promoted. And the bone resorption risk caused by micromotion is reduced.

This invention relates to the field of biomaterials technology, specifically to a dual-functional titanate fiber with both antibacterial and osteogenic properties, its preparation method, and its application. A hydrothermal method is used to pre-grow a uniformly distributed nanoscale fiber layer on the surface of a β-titanium alloy. The excellent bioactivity of the fiber surface layer promotes osteoblast growth and increases the rate of bone formation. Furthermore, to impart antibacterial properties to the fiber surface layer, different concentrations of europium (Eu) ions are introduced into the fiber surface layer after hydrothermal treatment using a cation exchange method. The excellent antibacterial properties of Eu ions are utilized to achieve the goal of modifying the surface layer to possess both excellent bioactivity and antibacterial properties.

The invention discloses a bone regeneration and repair titanium alloy with a macrophage aging adjusting effect and a preparation method and application thereof.The bone regeneration and repair titanium alloy comprises a titanium alloy matrix and a porous structure attached to the titanium alloy matrix, and the porous structure is in multi-stage size distribution and comprises long-strip-shaped gullies and large holes (50 [mu] m Cavity); the morphology in the gullies shows a coralline ligament and a nest-shaped pore channel (1 [mu] m) structure. This can provide space and sites for the growth and adsorption of osteoblasts. The invention also provides a novel method for preparing the non-oxidation and non-nitridation coralline-shaped porous structure on the commercial titanium implant through a vapor phase alloying (VPA) and vapor phase dealloying (VPD) combined method. And an effective way is provided for realizing in-vivo osseointegration and reconstruction.

This invention discloses a bioactive bone adhesive, its preparation method, and its applications. The adhesive comprises a bioactive glass component as a functional enhancement, which, through ligand-ion interactions, is composited with tetracalcium phosphate, O-phospho-L-serine, and polyacrylic acid. During the curing process, this invention effectively regulates the local acidic environment and stabilizes the system pH in a slightly alkaline range after 7 days, which is beneficial for osteoblast activity and bone tissue regeneration. After 24 hours of culture in simulated body fluid, the initial adhesion strength to titanium alloys reached 7.53 MPa; after 7 days in a humid environment, the compressive strength remained at 106.1 MPa. This invention also exhibits excellent in vitro mineralization capacity, cell compatibility, and osteogenic activity, with overall performance significantly superior to conventional bone repair materials, showing broad clinical application prospects in fracture fixation and bone defect repair.

The invention discloses an osteogenesis-promoting antibacterial dual-characteristic material and a preparation method thereof, the method comprises the following steps: S1, adding HA into analytically pure absolute ethyl alcohol, and mixing to obtain an HA ethanol dispersion liquid; s2, adding PEEK powder into the HA ethanol dispersion liquid, and mixing, so as to obtain HA-PEEK-ethanol blended liquid; s3, AgNPs powder is added into the HA-PEEK-ethyl alcohol blending liquid, mixing is carried out, and an HA-AgNPs-PEEK-ethyl alcohol blending liquid is obtained; s4, stirring the HA-AgNPs-PEEK-ethanol blending solution until the weight of the HA-AgNPs-PEEK-ethanol blending solution is not reduced any more, so as to obtain HA-AgNPs-PEEK powder; s5, the obtained HA-AgNPs-PEEK powder is dried and extruded, and a HA-AgNPs-PEEK wire rod is obtained; and S6, the HA-AgNPs-PEEK wire is dried, and the material with the osteogenesis promoting and antibacterial dual characteristics is obtained. According to the biological material provided by the invention, the HA and the AgNPs are added into the PEEK material, the mineralization ability of osteoblasts on the surface of the material can be remarkably enhanced by adding the HA and the AgNPs, the surface roughness of an osteogenic differentiation marker and the osteogenic material is up-regulated, meanwhile, the antibacterial property of the surface of the material is improved by the AgNPs, and cell climbing and adhesion during implantation of the osteogenic material in an infection environment are facilitated.

This invention relates to the field of medical material manufacturing technology, and more specifically, to a photothermally controllable antibacterial titanium alloy implant and its preparation method. The implant substrate is titanium or a titanium alloy, and its surface is activated by alkaline heat treatment. Polydopamine and copper ions are polymerized in situ at room temperature to coat the implant substrate surface. The implant obtained by this invention has no significant toxicity to osteoblasts, excellent biocompatibility, and combines multiple functions such as synergistic antibacterial activity, high hydrophilicity, osteopromoting properties, photothermally controllable properties, and safe sustained release. It can be widely applied in medical implantation fields such as orthopedic implants, bone fixation devices, and cardiovascular stents, providing a safe, efficient, and feasible new solution for effectively addressing key issues such as clinical infection and poor osseointegration in medical titanium alloys.

The invention belongs to the field of biomedical polymer material and additive manufacturing, and discloses a fused deposition modeling printed ferroferric oxide functionalized biodegradable polymer nano composite bone scaffold with near-infrared light / magnetic field activated shape memory and thermal therapy functions, PLA is used as a matrix, TBC is used for adjusting the glass transition temperature, Fe3O4 nano particles are doped, and the composite bone scaffold is prepared. And carrying out fused deposition modeling printing preparation. Fe3O4 endows the stent with near-infrared light / magnetic field dual response, wireless control shape memory is achieved, the compressed and implanted stent recovers the form in vivo to adaptively fill complex bone defects, and meanwhile controllable local high temperature is generated through the photo-thermal / magnetothermal effect to inhibit tumor cells. The stent has a communicated porous structure, and the compression strength is matched with cancellous bone; tBC improves hydrophilicity and degradability, Fe3O4 degrades and releases iron ions, and proliferation, differentiation and biomineralization of osteoblasts are promoted synergistically. The stent has minimally invasive, tumor treatment and bone repair capabilities, and provides a new strategy for complex bone defects.

The invention relates to a preparation method of a titanium dental implant surface coating with antibacterial and osteogenesis promoting properties, and belongs to the technical field of dental implant materials. According to the method, the double-layer functional synergistic coating is constructed on the surface of a titanium substrate through a three-step composite process including titanium substrate pretreatment, preparation of an inner-layer hydroxyapatite and chitosan composite coating and preparation of an outer-layer zinc-loaded mesoporous silane coating. An inner layer forms a basic functional layer by utilizing osteogenic activity of hydroxyapatite and natural antibacterial property of chitosan, an outer layer is loaded with zinc ions through a slow-release structure of mesoporous silane, long-acting controllable release of antibacterial components is realized, and meanwhile, the proliferation and differentiation capacity of osteoblasts is synergistically improved by the zinc ions and the mesoporous silane. The technical problems that an existing titanium dental implant coating is single in function, poor in antibacterial durability, insufficient in osteogenesis promoting effect, weak in binding force between the coating and a matrix and complex in preparation process are solved. The prepared coating is firmly combined with the titanium substrate, is high in biological safety, is simple and controllable in process, is suitable for industrial production, and has an important application value in the field of dental implant.