66 results about "Osteoblastic cell" patented technology

The invention discloses a biological compound material based on fish skin collagen and a preparation method of the biological compound material. The biological compound material consists of collagen, calcium nitrate and diammonium hydrogen phosphate by a certain ratio. The preparation method comprises the following steps: A, extracting the fish skin collagen; B, in-situ compounding the collagen and hydroxyapatite; C crosslinking the biological compound collagen material. The obtained biological compound collagen material has the advantages of good biological compatibility, reasonable formula, convenient operation and reduction in environment pollution. The method is easy to realize and simple to operate, a safe collagen extracting method is adopted, the compound material is synthesized through the proper proportioning of the collagen, the calcium nitrate and the diammonium hydrogen phosphate, and the biological compound collagen material has certain mechanical performance and excellent biological compatibility. A proper growth environment can be provided for the growth of bone cells, and the biological compound collagen material is a potential substitute for clinic bone materials.

The invention discloses a method for preparing a two-stage micron-submicron microstructure on the surface of a titanium alloy dental implant. The titanium alloy implant is activated, and is processed through surface modification such as direct current electrolytic etching and alternating current etching, so that a two-stage micron-submicron microstructure shape can be formed on the surface of the implant. The two-stage micron-submicron microstructure shape is prepared on the surface of the titanium alloy dental implant, so that the method achieves the property of guiding the adhesion of osteoblasts, can simulate natural histomorphology in vivo, can guide the bone matrix formation on the surface rapidly, and can promote the formation of good synosteosis. Therefore, the titanium alloy dental implant can promote the formation of new bones, can short the healing time, and can achieve the ideal effect that the titanium alloy dental implant can take on the recovery function as soon as being implanted further.

A preparation method for forming a calcium-containing nanosheet film layer on the surface of sandblasted and acid-etched titanium includes the following steps that (A) pure titanium is polished, sandblasted, subjected to ultrasonic cleaning and dried; (B) the pure titanium is acid-etched with hydrofluoric acid / nitric acid mixed liquid, subjected to ultrasonic cleaning with double distilled water and dried, and then acid-etched with concentrated hydrochloric acid / concentrated sulfuric acid mixed acid, subjected to ultrasonic cleaning with double distilled water and dried, so that a micron structure is formed on the surface of the titanium; and (C) the sandblasted and acid-etched pure titanium is subjected to a calcium hydroxide and hydrogen peroxide hydrothermal solution reaction for 0.5-24 hours under a water bath at the temperature of 70-90 DEG C, the calcium-containing nanosheet film layer is formed, and the titanium is processed for 1-3 hours at the temperature of 300-500 DEG C. By means of the surface nanosheet film layer and the calcium element contained in the prepared sample, the compatibility and combining capacity of the prepared sample with bones can be enhanced, adhesion, proliferation and differentiation of osteoblasts on the surface of the titanium are facilitated, and thus the osseointegration capacity of the titanium is improved. Meanwhile, the method is simple in process, processing is easy, and the preparation cost is low.

The invention discloses a multi-component bone tissue engineering scaffold and a preparation method thereof. The multi-component bone tissue engineering scaffold is prepared from chitosan, gelatin, pectin and nano hydroxyapatite by weight percent. The preparation method comprises the following steps of: selecting the chitosan and the pectin to prepare a hydroxyapatite / chitosan-pectin compound by virtue of a codeposition technology, wherein the particle diameter of the hydroxyapatite is 10-100nm; and dispersing nHCP into a chitosan-gelatin solution, crosslinking with glutaric dialdehyde and then freeze-drying to obtain the multi-component bone tissue engineering scaffold. In the scaffold prepared by the preparation method disclosed by the invention, nHCP can be uniformly dispersed in the scaffold and is difficult to agglomerate; the pore diameter of the scaffold is 100-300 mu m, and the porosity of the scaffold is greater than 80%; and the scaffold disclosed by the invention can be used for promoting the adhersion and growth of skeletogenous cells, has good biocompatibility and is expected to be used as a novel bone tissue engineering biological material.

The invention relates to a preparation method of a wood-based bionic bone scaffold material, and belongs to the field of bionic bone scaffolds. The method comprises the following steps: soaking wood fibers in an alkali solution, and performing mechanical grinding to obtain fiber pulp; mixing the fiber pulp with a polysaccharide polymer solution, and performing uniform stirring to obtain a mixed solution; adding hydroxyapatite into the mixed solution, and performing magnetic stirring in a water bath to obtain a scaffold material precursor mixed solution; freezing the scaffold material precursormixed solution, and performing freeze-drying to obtain an aerogel scaffold; performing cross-linking treatment on the aerogel scaffold with a glutaraldehyde solution, and performing rinsing with ethanol and deionized water; and freezing the rinsed scaffold material, and then performing freeze-drying to obtain the wood-based bionic bone scaffold material. According to the invention, the wood-basedbionic bone scaffold material with a three-dimensional porous structure is prepared by a circulating freeze drying technology. The scaffold material has no cytotoxicity and has high compressive strength; and mouse osteoblasts can adhere and grow on the scaffold material, so that requirements of a bone scaffold material are met.

Follistatin-like protein (FSTL-1) is a secreted glycoprotein of unknown function, first isolated from mouse osteoblastic cells as a transforming growth factor-β1-inducible gene. The inventors have discovered that FSTL-1 is a proinflammatory mediator. As such, the invention provides for composition and methods of using agents that bind to FSTL-1 to modulate various types of inflammation (e.g., autoimmune diseases). Inhibitors and antagonists of FSTL-1, particularly antibodies or antibody fragments, may be used to treat conditions related to inflammation, such as arthritis. In addition, the inventors have discovered that FSTL-1 has a role in the Th17 pathway. Accordingly, the invention provides for compositions and methods of using agents which bind to FSTL-1 to modulate the generation of Th17 cells. Such agents are useful for delaying development of and treating diseases associated with undesired production of Th17 cells, such as autoimmune diseases. Furthermore, since FSTL-1 is a proinflammatory mediator with a role in cancer, the invention provides for compositions and methods of using a pharmaceutical composition of FSTL-1 to delay development of or treat cancer.

A PHSRN-RGD-containing oligopeptide and a composition for promoting bone formation, which contains such oligopeptide as an effective ingredient. The oligopeptide promotes osteoblastic cell adhesion and differentiation and enhances bone regenerative ability, so that the inventive oligopeptide can be effectively used in regenerative treatment of bone tissue and periodontal tissue.

The invention discloses a method for preparing a bionic skeletal muscle composite tissue through multi-channel extrusion 3D biological printing. The preparation method comprises the following steps: preparing bone scaffold bionic bio-ink, periosteum bionic bio-ink, sarcolemma bionic bio-ink and muscle bionic bio-ink; respectively mixing MSCs and C2C12 with the corresponding bionic bio-inks; and printing and forming a bionic bone, bionic periosteum, bionic sarcolemma and bionic muscle four-layer composite tissue engineering scaffold by using a multi-channel extrusion 3D biological printer. Themethod for preparing the bionic skeletal muscle composite tissue through multi-channel extrusion 3D biological printing can minimize fibrosis during traumatic skeletal muscle injury recovery; the bionic skeletal muscle composite tissue prepared through multi-channel extrusion 3D biological printing can replace structures and functions of bones and skeletal muscles at the same time, and supports proliferation and differentiation of myoblasts and osteoblasts; and an implant is easy to customize by utilizing a 3D biological printing technology, so that the implant is suitable for any defect shape.

The invention discloses a method for preparing a collagen substrate material for an oral cavity by using biological bone. The method comprises the steps of cutting young bovine bone or swine bone, then, carrying out treatments such as virus removing, defatting, deproteinization, decalcification, cell removing and endotoxin removing, then, carrying out freeze-drying, and then, shearing the bone into required specifications. The whole process is simple, the production cycle is short, and thus, large-scale mass production is facilitated. According to the method, the young bovine bone or swine bone is selected as a raw material and is rich in source, degradation-induced inflammatory reactions are reduced, and clearing is easy, so that immunological rejections caused after human body implantation are avoided. Moreover, the young bovine bone and swine bone materials have good porosity and pore size channels and are similar to human bones in porosity, a human body microenvironment is simulated, crawling passing of seed cells such as osteoblasts is facilitated, healing of soft tissue can be excellently promoted, and the osteogenesis capability is relatively good. Furthermore, chemical reagents of all treatment steps are spin-dried in a long running water state and washed off, the speed is high, and the washing is thorough and residual-free, so that the safety of use of the product is further guaranteed.

The present invention provides methods for obtaining mesenchymal stem cell (MSC)-derived cells of chondro-osteoblastic lineage from MSC. The invention also relates to a population of MSC- derived cells of chondro-osteoblastic lineage obtained by the methods, a pharmaceutical formulation comprising the population of MSC-derived cells of chondro-osteoblastic lineage, and their use in the treatment of a subject in need of transplantation of cells of chondro-osteoblastic lineage.