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Biological compound material based on fish skin collagen and preparation method of biological compound material

A fish skin collagen and composite material technology is applied in the field of biocomposite materials and the preparation of biocomposite materials. , the effect of increasing added value

Inactive Publication Date: 2014-04-02
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In existing studies, most materials used for bone replacement are mechanical mixtures of hydroxyapatite and polymers. Does not fuse well and its structure is difficult to resemble natural bone

Method used

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  • Biological compound material based on fish skin collagen and preparation method of biological compound material
  • Biological compound material based on fish skin collagen and preparation method of biological compound material
  • Biological compound material based on fish skin collagen and preparation method of biological compound material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A biocomposite material based on fish skin collagen, which is made of the following raw materials in parts by weight:

[0057] Raw material parts by weight

[0058] Collagen 25

[0059] Calcium nitrate 58.80

[0060] Diammonium hydrogen phosphate 19.73

[0061] A preparation method based on a biocomposite material of fish skin collagen, the steps of which are:

[0062] a, the fish skin is processed to remove fat and miscellaneous proteins in the fish skin;

[0063] b. Stir fish skin and 0.5mol / L acetic acid at a ratio of W / V=1:40 at low speed for 22 or 23 or 24 or 25 or 26 hours at 4°C. After 22 or 23 or 24 or 25 or 26 hours, centrifuge at 5000r / min for 9 or 10 or 11 minutes at 4°C, take the supernatant, and extract the unextracted fish skin with the same amount of reagent again;

[0064] c. Add a certain amount of 5mol / L sodium chloride acetic acid (0.5mol / L) solution to the collagen solution extracted in step b and stir until the final salt concentration is 0.9mo...

Embodiment 5

[0075] A preparation method based on a biocomposite material of fish skin collagen, the steps of which are:

[0076] a) At 4°C, add the acid-soluble collagen obtained by extraction and purification into 0.5% by mass acetic acid solution and stir to prepare a collagen solution.

[0077] b) After the collagen is dissolved, adjust the pH to 5.0. Take an appropriate amount of collagen solution, and add calcium nitrate and diammonium hydrogen phosphate at a concentration of 1 mol / L dropwise, respectively. The mass ratio of collagen to synthetic hydroxyapatite was 50:50.

[0078] c) Adjust the pH of the solution to about 7.5 with 10% by mass ammonia water. Then the mixed solution was placed at 4° C. and stirred for 2 h. Then place it in a constant temperature incubator at 37°C for aging for 24 hours, and wash thoroughly with ultrapure water to neutralize the precipitate. The mixed solution was added dropwise into a 24-well plate, frozen at -70°C for 4 hours, and then freeze-drie...

Embodiment 6

[0081] A preparation method based on a biocomposite material of fish skin collagen, the steps of which are:

[0082] a) Add the extracted and purified acid-soluble collagen to 0.5% (mass ratio) acetic acid solution and stir at 4°C to prepare a collagen solution.

[0083] b) After the collagen is dissolved, adjust the pH to 5.0. Take an appropriate amount of collagen solution, and add calcium nitrate and diammonium hydrogen phosphate at a concentration of 1 mol / L dropwise, respectively. The mass ratio of collagen to synthetic hydroxyapatite is 30:70.

[0084] c) Adjust the pH of the solution to about 7.5 with a 10% mass ratio ammonia solution. Then the mixed solution was placed at 4° C. and stirred for 2 h. Then place it in a constant temperature incubator at 37°C for aging for 24 hours, and wash thoroughly with ultrapure water to neutralize the precipitate. The mixed solution was added dropwise into a 24-well plate, frozen at -70°C for 4 hours, and then freeze-dried.

[0...

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Abstract

The invention discloses a biological compound material based on fish skin collagen and a preparation method of the biological compound material. The biological compound material consists of collagen, calcium nitrate and diammonium hydrogen phosphate by a certain ratio. The preparation method comprises the following steps: A, extracting the fish skin collagen; B, in-situ compounding the collagen and hydroxyapatite; C crosslinking the biological compound collagen material. The obtained biological compound collagen material has the advantages of good biological compatibility, reasonable formula, convenient operation and reduction in environment pollution. The method is easy to realize and simple to operate, a safe collagen extracting method is adopted, the compound material is synthesized through the proper proportioning of the collagen, the calcium nitrate and the diammonium hydrogen phosphate, and the biological compound collagen material has certain mechanical performance and excellent biological compatibility. A proper growth environment can be provided for the growth of bone cells, and the biological compound collagen material is a potential substitute for clinic bone materials.

Description

technical field [0001] The invention relates to a biocomposite material, more specifically to a biocomposite material based on fish skin collagen, and also relates to a preparation method of the biocomposite material based on fish skin collagen, belonging to the field of biomedical materials. In the present invention, hydroxyapatite is embedded in the collagen matrix, and the synthesized collagen composite biological material has the advantages of high biodegradability, large surface energy, good biological activity and good compatibility, and is a potential natural bone repair The material can be applied to bone tissue engineering as a clinical bone material substitute. Background technique [0002] Collagen has its unique natural triple helical structure and properties, and collagen fibers are the main fibers of animal skin tissue. Due to their special three-dimensional network structure, they have a wide range of uses. Collagen is an important protein that constitut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/12A61L27/02
Inventor 喻亚丽周运涛何力甘金华吕磊庞昆
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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