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Method of accelerating decomposition of maize straw retention

A technology of corn stalks and corn stalk powder, which is applied in the field of accelerating the decomposition of corn stalks, which can solve the problems of difficulty in colonization, infecting the inside of straws, and insufficient contact with bacteria agents, so as to accelerate the process of returning to the field and promote decomposition , the effect of broad application prospects

Active Publication Date: 2014-08-27
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also some restrictive factors in the use of straw-degrading bacteria agents, such as the inability of the bacteria agent, water and straw to fully contact, and the difficulty in colonizing the waxy layer of the straw with the bacteria agent and infecting the inside of the straw, etc.
This makes the effect of the degrading bacteria agent not fully exerted, and the decomposition rate of the straw is limited

Method used

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  • Method of accelerating decomposition of maize straw retention
  • Method of accelerating decomposition of maize straw retention
  • Method of accelerating decomposition of maize straw retention

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The degrading bacteria were screened from the soil of rotten leaves in Zijin Mountain, Nanjing. The suspension of the soil samples diluted step by step was applied to the CMC-Congo red plate by the gradient dilution coating method, and cultured at 30°C to screen the cellulose-degrading bacteria. The formula of the above-mentioned CMC-Congo red medium is: sodium carboxymethylcellulose (CMC-Na) 10g, (NH 4 ) 2 SO 4 2g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O0.5g, Congo red 0.2g, agar 20g, water 1000mL, autoclave at 121℃ for 20min.

[0029] Purify and isolate strains that produce larger transparent circles and grow faster on the CMC-Congo red plate, and then inoculate them into a rice straw powder medium with rice straw as the only carbon source (the formula of the rice straw powder medium is: Dry crushed rice straw powder 10g, KH 2 PO 4 3.0g; NaNO 3 3.0g; CaCl 2 0.5g; MgSO 4 ·7H 2 O0.5g, FeSO 4 ·7H 2 O0.0075g, MnSO 4 ·H 2 O0.0025g, ZnSO 4 0.002g, CoCl 2 0.003g, 20g...

Embodiment 2

[0031] Inoculate the strain NJGZ-2 into a conical flask containing 50 mL of spore-forming medium, culture it statically at 30°C for 7 days, then add 30 mL of sterile water and shake it on a shaker for 30 minutes, filter the culture with sterile gauze to get the spore suspension liquid, adjust the spore concentration in the spore suspension to 10 with sterile water 7 cfu / mL, the straw-degrading bacterial agent is obtained.

[0032] The preparation of the sporulation medium: 10.0 g of corn straw powder, 5.0 g of peptone, 2.0 g of yeast powder, 0.3 g of urea, 1000 mL of water, natural pH, autoclaving at 121° C. for 20 min.

[0033] The corn stalk powder is the corn stalk powder obtained by drying the corn stalks until the water content is below 1%, crushing and passing through a 30-mesh sieve.

Embodiment 3

[0035] The effects of the method of the present invention and the method of directly returning straw to the field on the decomposition of corn straw were compared through a pot plant simulation test.

[0036]The auxiliary agent is polyethylene glycol p-isooctyl phenyl ether, take 1mL polyethylene glycol p-isooctyl phenyl ether, add water to prepare an auxiliary agent with a concentration of polyethylene glycol p-isooctyl phenyl ether of 0.1% solution.

[0037] Pot simulation test: cut corn stalks into 1-2cm corn stalks, take 10g of corn stalks and put them into a 100-mesh nylon mesh bag of 10cm×15cm. In a flowerpot filled with soil, 3cm deep from the soil surface, control the soil water content to 60%-80% of the field water holding capacity during the test (approximately add 25mL of water per 100g of soil), and cultivate it statically at 20-30°C for 30 Days later, the degradation rate of corn stalks was measured.

[0038] Determination of the degradation rate of corn stalks:...

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Abstract

The invention belongs to the field of crop straw utilization and discloses a method of accelerating decomposition of maize straw retention. The method comprises the following steps: crushing maize straws; mixing with an assistant; inoculating a straw degrading bacterial agent; burying the straws in soil; controlling the water content of the soil to 60-80% of field water retention; and decomposing at 20-30 DEG C. Pot experiments verify that the method provided by the invention has the best decomposing effect on maize straws and the degradation rate of the straws reaches 51.9% which is improved by 14% compared with that of direct retention of the straws. The TR assistant can remarkably accelerate the straw degrading bacterial agent to generate cellulose, and the filter paper enzyme (FPA), the cellulase activity (CMCase) and the activity of hemicellulase (Xylanase) are greatly enhanced, so that the straws are directly promoted to decompose. The method disclosed by the invention promotes decomposition of maize straws and accelerates the retention process of the maize straws, can be used for conversion and utilization of straw wastes and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of crop straw utilization, and relates to a technical method for resource utilization of straw waste, in particular to a method for accelerating the decomposition of corn straw returning to the field. Background technique [0002] Our country has more than 200 million tons of corn stalks every year, ranking first in the world. Traditionally, straw is mostly used as fuelwood in rural areas or dumped into the environment in the form of accumulation and waste burning. Nowadays, the increasingly severe smog in China is closely related to the wanton burning of straw. At the same time, straw can be used as a kind of high-quality available resources due to its rich organic carbon and rich mineral elements such as nitrogen, phosphorus, and potassium. Therefore, the efficient resource utilization of straw can not only reduce pollution and protect the environment, but also turn waste into treasure and achieve sustainable devel...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 徐阳春樊晓腾韦中沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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