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Method for preparing antibacterial titanium material having good biocompatibility and containing silver on surface

A biocompatible, surface-containing technology, applied in the field of materials, can solve problems such as cytotoxicity, and achieve the effects of simple operation, strong controllability, and good biocompatibility

Inactive Publication Date: 2018-08-24
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the release of large amounts of Ag in a short period of time can cause cytotoxicity

Method used

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  • Method for preparing antibacterial titanium material having good biocompatibility and containing silver on surface
  • Method for preparing antibacterial titanium material having good biocompatibility and containing silver on surface
  • Method for preparing antibacterial titanium material having good biocompatibility and containing silver on surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] A method for preparing an antibacterial titanium material containing silver on the surface with good biocompatibility, characterized in that it comprises the following steps:

[0070] 1) A titanium dioxide nanotube array (TNT) with a diameter of 70nm was constructed on the surface of the titanium material by anodic oxidation, immersed in a silver nitrate solution, and silver nanoparticles (TNT) were loaded into the titanium dioxide nanotubes by ultraviolet reduction. -Ag).

[0071] 1.1) After drying the cleaned titanium foil (10mm×10mm), the electrochemical corrosion process is carried out;

[0072] The cleaning process is as follows: the titanium foil is sequentially cleaned with ethanol, acetone, ethanol and distilled water for 15 minutes respectively.

[0073] In the drying process: the temperature is 60°C, and the time is 4 hours;

[0074] In the electrochemical corrosion process: the platinum foil is used as the cathode, and the dried titanium foil is used as the...

experiment example 1

[0117] Release of silver ions, evaluation of biocompatibility of materials

[0118] In this experimental example, two different samples, TNT-Ag and LBL, were immersed in phosphorylation buffer (PBS) to study the release of silver ions in the material; and to evaluate the LDH level of osteoblasts on the surface of different materials, the Activity and cell adhesion, specifically including the following steps:

[0119] 1) In order to verify the effect of the multilayer film on the release of silver ions, 5 samples of each group (TNT-Ag and LBL) were soaked in 5mL PBS;

[0120] After incubation (37° C.) for different times (1, 4, 7, 14, 28 days), 5 mL of leachate was taken from each group of samples to detect the release rate of silver ions.

[0121] The release of silver ions in this experiment was measured by ICP-AES;

[0122] from Figure 4 It can be seen that no matter at any time point, the silver ions released by the LBL sample are lower than that of the TNT-Ag sample, s...

experiment example 2

[0134] Bacterial adhesion, antibacterial rate and bacterial activity detection on material surface

[0135] Dilute Staphylococcus aureus and Escherichia coli in 1×10 6 The density of each well was inoculated on the surface of different materials. After culturing at 37°C for 6 hours, the nucleoids were stained with Hochest33258 for 5 minutes. In addition, the antibacterial effect of different material surfaces relative to pure titanium surfaces after 6 hours of culturing was also tested. Rate.

[0136] 1) In this test, in order to prepare the CLSM observation sample, the bacteria were first fixed with 4% paraformaldehyde solution at 4°C for 30 minutes, and then Hochest33258 was used to stain the bacteria at 4°C for 5 minutes. , and then CLSM observes the bacterial adhesion on the surface of different materials;

[0137] 2) In order to test the anti-bacterial adhesion efficiency on the surface of different materials, different titanium materials were taken out, gently rinsed w...

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Abstract

The invention discloses a method for preparing an antibacterial titanium material having a good biocompatibility and containing silver on the surface. The method comprises the following steps: constructing uniform titanium dioxide nanotubes on the surface of a titanium material by an anodization technology, used as a silver nanoparticle store; and oxidizing sodium alginate by sodium periodate to obtain dialdehyde sodium alginate denoted as ADA and used as an anionic polyelectrolyte, and performing layer-by-layer self-assembling on the anionic polyelectrolyte and cationic polyelectrolyte CHI toform a polyelectrolyte multilayer film. The driving force for forming the multilayer film is derived from the electrostatic interaction between the ADA and the CHI and the covalent interaction between the aldehyde group on the ADA and the amino group on the CHI, so the constructed stable multilayer film can effectively control the release of silver ions, reduce the toxicity of the silver ions toosteoblasts and achieve good biocompatibility, and also can achieve excellent antibacterial properties in order to make a titanium-based implant have good antibacterial and osteogenic properties.

Description

technical field [0001] The invention relates to the field of materials, in particular to a method for preparing an antibacterial titanium material containing silver on the surface and having good biocompatibility. Background technique [0002] Titanium and titanium alloys have excellent mechanical strength, biocompatibility and corrosion resistance, and are the most commonly used biomaterials in dental and orthopedic treatments. However, postoperative infection associated with biomaterials remains an important challenge for clinical application. Infections after implantation can lead to delayed wound healing and even require repeated surgeries, which cause pain to the patient as well as excessive costs. What's more, once the bacteria attach to the implanted titanium surface, they will form a biofilm to resist the lethal effect from the immune system, and antibiotic treatment is very ineffective in killing the bacteria that have formed the biofilm. Inhibition of early bacte...

Claims

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Application Information

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IPC IPC(8): A61L27/30A61L27/06A61L27/34A61L27/54A61L27/50C25F3/08
CPCA61L27/06A61L27/306A61L27/34A61L27/50A61L27/54A61L2300/104A61L2300/404A61L2300/602A61L2400/12A61L2400/18A61L2420/02A61L2420/06A61L2420/08C25F3/08C08L5/04C08L5/08
Inventor 蔡开勇袁璋刘鹏
Owner CHONGQING UNIV
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