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A cultivation method for rapidly obtaining regenerated seedlings of Lycium barbarum

A technology of black fruit wolfberry and cultivation method, which is applied in the field of tree biology, can solve the problems of low bud formation rate and long cultivation time, and achieve the effects of reducing cultivation cost, fast growth speed and strong root system

Active Publication Date: 2020-02-21
NANJING XIAOZHUANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, the technical problem to be solved in the present invention is to overcome the defects of long cultivation time and low bud formation rate obtained by explants in the prior art to obtain regenerated seedlings of Lycium ruthenicum, thereby providing a method for rapidly obtaining regenerated Lycium ruthenicum. Seedling cultivation method

Method used

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  • A cultivation method for rapidly obtaining regenerated seedlings of Lycium barbarum
  • A cultivation method for rapidly obtaining regenerated seedlings of Lycium barbarum
  • A cultivation method for rapidly obtaining regenerated seedlings of Lycium barbarum

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0047] Experimental example 1: MS medium configuration

[0048] S1: MS medium stock solution configuration

[0049] Contrast Table 1-4, weigh each component respectively.

[0050] Table 1: MS macroelement mother liquor formula

[0051]

[0052] After weighing, dissolve thoroughly with a small amount of distilled water and mix, and finally make up to 1L.

[0053] Table 2: MS trace element mother liquor formula

[0054]

[0055] After weighing, dissolve thoroughly with a small amount of distilled water and mix, and finally make up to 1L.

[0056] Table 3: MS inositol master solution formula

[0057]

[0058] After weighing, dissolve thoroughly with a small amount of distilled water and mix, and finally make up to 1L.

[0059] Table 4: Formula of MS iron salt mother liquor

[0060]

[0061] After weighing, dissolve thoroughly with a small amount of distilled water and mix, and finally make up to 1L.

[0062] S2: MS medium configuration

[0063] Dilute the MS t...

experiment example 2

[0064] Experimental example 2: The configuration of hormone NAA and 6-BA

[0065] Weigh 0.04g of NAA powder, dissolve it with 1ml of absolute ethanol, and then set the volume to 200ml to obtain a 0.2mg / ml NAA solution, and store it in a refrigerator at 4°C for later use.

[0066] Weigh 0.04g of 6-BA powder, dissolve it with 1mol / L dilute sodium hydroxide solution, and then set the volume to 200ml to obtain a 0.2mg / ml 6-BA solution, and then store it in a refrigerator at 4°C for later use.

Embodiment 1

[0068] 1. Disinfection of seeds

[0069] Select black wolfberry seeds, soak them in 70v% alcohol for 5 minutes, then disinfect them with 0.1wt% mercuric chloride solution for 15 minutes, and then rinse them three times with sterile water.

[0070] 2. Obtaining sterile seedlings

[0071] Add 30g sucrose and 8.0g agar to each liter of MS medium, adjust the pH value to 5.8 with 1mol / L NaOH solution, and then sterilize under high pressure at 121°C for 15min to obtain MS-0 medium, at this time the pH of the medium The value is 5.2.

[0072] The seeds in step 1 are placed in MS-0 medium, and sterile seedlings are obtained after the seeds germinate for 8-12 days.

[0073] 3. Induction of Callus

[0074] Add 1.0 mg NAA, 2.0 mg 6-BA, 30 g sucrose and 8.0 g agar to each liter of MS medium, adjust the pH value to 5.8 with 1 mol / L NaOH solution, and then sterilize under high pressure at 121°C for 15 minutes to obtain MS-0 Culture medium, the pH value of the culture medium at this time...

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Abstract

The invention belongs to the technical field of forest creatures, and discloses a cultivation method for quickly obtaining lycium ruthenicum regeneration seedlings. The cultivation method comprises the following steps: disinfection of seeds, obtaining of germfree seedlings, induction of a callus, obtaining of adventitious buds and induction of roots. According to the cultivation method, the callus is directly obtained from the germfree seedlings of lycium ruthenicum, and the lycium ruthenicum regeneration seedlings are cultivated through a tissue culture method; compared with a conventional method for inducing the callus through leaves or stems of the seedlings, the cultivation method has the advantages that the tissue culture time is greatly shortened, and cultivation cost of the lycium ruthenicum is greatly reduced; meanwhile, the cultivation efficiency is effectively improved, and the transplanting survival rate of the regeneration seedlings reaches up to 98 percent. The lycium ruthenicum obtained through the cultivation method has the characteristics of being high in genetic stability, low in cost, short in seedling growing period, high in reproduction coefficient, and easy and convenient to operate.

Description

technical field [0001] The invention belongs to the technical field of forestry biology and relates to a plant cultivation method, in particular to a cultivation method for rapidly obtaining regenerated seedlings of Lycium barbarum. Background technique [0002] Lycium Ruthenicum Murr (Lycium Ruthenicum Murr) is a perennial thorny shrub of Lycium genus Solanaceae (Solanceae). It is one of the important drought-resistant and saline-alkaline desert pioneer tree species. , Northern Xinjiang, Neiling, riverbed beaches and other environments with very harsh conditions are scattered. [0003] Wild black wolfberry is sweet in taste and flat in nature, rich in protein, fat, sugar, free amino acid, organic acid, mineral, trace element, alkaloid, vitamin C, B1, B2, calcium, magnesium, copper, zinc, manganese, Iron, lead, nickel, cadmium, cobalt, chromium, potassium, sodium and other nutrients. According to scientific testing, the content of calcium, iron and nicotinic acid in black ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01C1/00
CPCA01C1/00A01H4/001A01H4/008
Inventor 王立科毛善国陈全战杨平张边江唐宁
Owner NANJING XIAOZHUANG UNIV
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