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A kind of northern valerian tissue culture propagation method

A technique of tissue culture and northern valerian, applied in horticultural methods, botany equipment and methods, horticulture, etc., to achieve the effects of high seedling survival rate, stable system, and easy rooting and cultivation

Active Publication Date: 2017-01-11
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the tissue culture of Valeriana plants. There are reports of using cotyledons to induce bud clusters under sterile conditions. At the same time, adventitious roots are only induced from leaves of V. Breeding Research Reports

Method used

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  • A kind of northern valerian tissue culture propagation method
  • A kind of northern valerian tissue culture propagation method
  • A kind of northern valerian tissue culture propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Material:

[0039] The test material, the tissue culture explant, was the young stem segment of the Valeriana plant.

[0040] 2. Experimental steps:

[0041] (1) Explant induction: Rinse the young stems with tap water for 20 minutes, sterilize with 70% ethanol for 1 minute, sterilize with 1% sodium hypochlorite solution for 15 minutes, then take out, rinse with sterile water 4 times, and inoculate at 1.0 mg / L Cultivate on 6-BA, 0.02mg / L IBA MS medium, add 3% sucrose, 8g / L agar to the medium, pH 5.8, culture temperature 25℃, light time 16h·d -1 , 3000lux light;

[0042] (2) Subsequent proliferation culture: the adventitious buds formed in step (1) were cut under aseptic conditions and inoculated into 1.0 mg / L 6-BA, 0.02 mg / L IBA and 8 g / L agar with pH 5.8 MS Proliferation experiments were carried out on the proliferation medium to obtain the cluster buds for subsequent proliferation. The medium was added with 3% sucrose, 8g / L agar, pH 5.8, culture temperature was 25℃, and li...

Embodiment 2

[0056] 1. Material:

[0057] The test material, the tissue culture explant, was the young stem segment of the Valeriana plant.

[0058] 2. Experimental steps:

[0059] (1) Induction of explants: Rinse the young stems with tap water for 15 minutes, sterilize with 60% ethanol for 30 seconds, sterilize with 0.5% sodium hypochlorite solution for 10 minutes, then take out, rinse with sterile water 3 times, and inoculate 0.5mg / L Cultivate on 6-BA, 0.01mg / L IBA MS medium, add 2% sucrose, 8g / L agar to the medium, pH 5.0, culture temperature 20℃, light time 12h·d -1 ,Illumination 2500lux;

[0060] (2) Subsequent proliferation and culture: the adventitious buds formed in step (1) were cut under aseptic conditions and inoculated into 0.5 mg / L 6-BA, 0.01 mg / L IBA and 8 g / L agar with pH 5.8 MS The proliferation experiment was carried out on the proliferation medium to obtain the clumping buds for subsequent proliferation. The medium was added with 2% sucrose, 8g / L agar, pH 5.0, culture temperatur...

Embodiment 3

[0074] 1. Material:

[0075] The test material, the tissue culture explant, was the young stem segment of the Valeriana plant.

[0076] 2. Experimental steps:

[0077] (1) Induction of explants: Rinse the young stems with tap water for 25min, disinfect with 80% ethanol for 90s, disinfect with 1.5% sodium hypochlorite solution for 20min, take it out, rinse with sterile water 5 times and inoculate 0.2mg / L Cultivate on 6-BA, 0.01mg / L IBA MS medium, add 5% sucrose, 8g / L agar to the medium, pH 6, culture temperature 30℃, light time 20h·d -1 ,Illumination 4000lux;

[0078] (2) Subsequent proliferation culture: the adventitious buds formed in step (1) are cut under aseptic conditions, and inoculated into 0.2mg / L6-BA, 0.01mg / L IBA and 8g / L agar MS with a pH of 5.8 The proliferation experiment was carried out on the proliferation medium, and the clumping buds for subsequent proliferation were obtained. The medium was added with 5% sucrose, 8g / L agar, pH 6, culture temperature was 30℃, and lig...

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Abstract

The invention aims to provide a Valeriana Fauriei Briq. tissue culture breeding method. The method is as below: 1, using young stems of Valeriana Fauriei as explants and conducting aseptic treatment; 2, placing sterile Valeriana Fauriei stems on a solid culture medium to induce adventitious buds; 3, inoculating the adventitious buds onto the solid medium for rooting and seedling, induced; and 4, transplanting the tissue culture seedlings to the culture medium for hardening seedling cultivation. The invention is not limited or influenced by natural conditions and, has the characteristics of fastness and easiness for large scale. The invention can ease the problem of shortage of wild Valeriana Fauriei resources and of destruction of ecological balance caused by overexploitation.

Description

Technical field [0001] The present invention belongs to the technical field of plant tissue culture and propagation, and specifically relates to a method for tissue culture and propagation of Valeriana fauriei Briq. Background technique [0002] Valeriana fauriei Briq. (Valeriana fauriei Briq.) is a wild perennial herb of Valerianaceae Valeriana (Valeriana). Valerian has a flat taste and has the effects of calming and tranquilizing, antispasmodic and analgesic. The rhizome has been used as a sedative for thousands of years. Valerian is a representative of the Valerian genus, distributed in Northeast China (Heilongjiang, Jilin, Liaoning) and Eastern China (Jiangsu, Anhui, Zhejiang, Jiangxi, Taiwan), and extends west to Henan, Shaanxi and other places. Valeriana officinalis is born in hillside meadows, wetlands between forests, and roadside forests below 2000m above sea level. At present, the pharmacological effects and chemical constituents of Valeriana have been studied to vary...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张鑫宋经元辛天怡陈士林
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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