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Method for obtaining regenerated plants by inducing radicle adventitious buds of Camellia oleifera

A technology for plant regeneration and bud induction, which is applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., and can solve the problems such as the lack of explants for transgenic Camellia oleifera, the inability to use the Camellia oleifera genetic system for forwarding, and the inability to transgenic plants.

Active Publication Date: 2016-09-28
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many studies on the tissue culture of Camellia oleifera. Zhang Zhijun et al. used the cotyledons of Camellia oleifera clones to obtain regenerated plants through somatic embryogenesis. Li Ze et al. It is of great significance in cultivating rapid propagation, and cannot be applied to the transformation of Camellia oleifera genetic system to obtain transgenic plants
The most commonly used method for plant genetic system transformation is the Agrobacterium-mediated method to infect plant leaves, hypocotyls, petioles, radicles and other organs, while the induction rate of adventitious buds induced by tea leaves is only 17.86%, which cannot be applied to oil tea at present. In terms of genetic system forwarding, the Camellia oleifera transgene has not yet found a suitable explant

Method used

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  • Method for obtaining regenerated plants by inducing radicle adventitious buds of Camellia oleifera
  • Method for obtaining regenerated plants by inducing radicle adventitious buds of Camellia oleifera
  • Method for obtaining regenerated plants by inducing radicle adventitious buds of Camellia oleifera

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Experimental program
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Effect test

Embodiment 1

[0036] Do the following in order:

[0037] 1. Select mature raw camellia oleifera seeds of the same year, remove the seed coat, rinse the seeds with tap water for 3-5 minutes, then soak for 10-15 hours, change the water 2-3 times in the middle, and then use 75% of the water in the ultra-clean workbench Soak in alcohol for 20-30s, rinse with sterile water for 3-5 times, and then use 0.1% HgCl 2 Disinfect for 3-5 minutes, rinse with sterile water 5-6 times, cut off most of the endosperm, inoculate the seed embryo with endosperm in WPM basic medium, culture in dark for 2-3 days, then transfer to light for 20- Sterile vaccines can be obtained in 25 days; the culture temperature is 26±1°C, the light intensity is 2100-2200lx, and the light time is 12-14h / d.

[0038] Table 1 Effect of different disinfection treatment methods on the disinfection effect of Camellia oleifera germ

[0039] 75% alcohol 0.1% mercury liter pollution rate browning rate 0 2 83.23 0 ...

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Abstract

The invention belongs to the field of plant cell engineering and discloses a method for acquiring regeneration plants by adventitious bud inducement of camellia oleifera radicles. The method mainly includes steps of direct inducement of adventitious buds through camellia oleifera radicles , subculture multiplication, best seedling cultivation, rooting cultivation, acclimatization, transplantation and the like. Not only is an approach for rapid propagation of excellent camellia oleifera varieties provided but also a technical support is provided for shortening camellia oleifera breeding process and improving resistibility of camellia oleifera by means of gene engineering.

Description

technical field [0001] The invention belongs to the field of camellia oleifera cell engineering, and in particular relates to a method for inducing adventitious buds by using the camellia oleifera radicle as an explant, and then obtaining a complete test-tube seedling plant. Background technique [0002] Camellia oleifera (Camellia oleifera) is an evergreen shrub or small tree of the genus Camellia in the family Theaceae. The unsaturated fatty acid content of camellia oil is more than 90%. It has a good color and fragrance, unique flavor, rich nutrition, storage resistance, lowers blood pressure, blood lipids and softens blood vessels, and plays an important role in the prevention of cardiovascular diseases. It is a high-quality edible oil One of them is known as "Oriental Olive Oil". At the same time, the by-products of camellia oleifera are also high-quality raw materials for the production of fertilizers, pesticides, and tannins. Camellia oleifera is mainly distributed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李泽谭晓风卢锟张琳吴玲利林青袁军吕佳斌石凯
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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