Method for acquiring and propagating nervilia fordii aseptic rhizomes

Abstract

The invention discloses a method for acquiring and propagating nervilia fordii aseptic rhizomes. The method is characterized in that disinfected explants are arranged in induction culture media, adventitious bud induction is carried out on the disinfected explants, the explants germinate to obtain the aseptic rhizomes, 1/2 MS is used as a basic culture medium for the induction culture media, agarwith the concentration of 5g L<-1>, sucrose with the concentration of 30 g L<-1>, 6-benzyladenin with the concentration of 1.5 mg L<-1> and naphthylacetic acid with the concentration of 0.3 mg L<-1> are added into the basic culture medium, the pH (potential of hydrogen) values of the induction culture media are 6.8, and the culture conditions include the temperatures of 25-27 DEG C, the illumination intensity of 2100 lux, the illumination time of 10-12 h/d and the culture time of 15-20 d. The method for acquiring and propagating the nervilia fordii aseptic rhizomes has the advantages of high adventitious bud induction rate and applicability to factory production.

Description

technical field [0001] The invention relates to a method for obtaining and multiplying aseptic rhizomes of blue skyflower. Background technique [0002] Blue Sky Kwai is the leaf or bulbous leaf of orchid plant Nerviliafordii (Hance) Schitr, also known as unifoliate, uniped lotus, pearl leaf, falling jack, iron hat, samosa, and green lotus. Flat in nature, bitter in the mouth, sweet, cool. Astragalus is a rare and endangered plant in my country. It has the functions of clearing the lung and relieving cough, strengthening the spleen and eliminating stagnation, clearing away heat and detoxification, eliminating stagnation and dispelling pain. Scrofula, sores, swollen toxins, bruises, etc. The blue skyflower is mainly propagated by bulbs, but the reproduction coefficient is extremely low. In addition, the extinction-style excavation by people over the years has led to the depletion of wild blue skyflower resources. However, the demand in domestic and foreign markets is increa...

AI Technical Summary

Problems solved by technology

The blue skyflower mainly reproduces by bulbs, but the reproduction coefficient is extremely low. In addition, people have been digging extinctly for many years, which has led to the depletion of wild blue skyflower resources.

However, the demand in domestic and foreign markets is increasing year by year, and Qingtiankui is far from meeting the demand of the market.