Method for obtaining regenerated plant by utilizing camellia oleifera hypocotyl

A technology for regenerating plants and Camellia oleifera, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of transgenic Camellia oleifera without finding explants, unable to apply, and unable to transgenic plants, etc.
CN104938335BActive Publication Date: 2017-04-05CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Publication Date
2017-04-05

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Abstract

The invention discloses a method for obtaining regeneration plants by use of camellia oleifera hypocotyl and belongs to the field of plant cell engineering. The method mainly comprises the step that adventive buds are induced by camellia oleifera hypocotyl. Through multiplicative subculture, strong seedling culture, rooting culture, acclimatization and transplant and the like of the adventive buds, not only is a new way provided for quick propagation of favorable camellia oleifera plants, but also a solid foundation is laid for improving the resistivity of camellia oleifera, accelerating the breeding process of camellia oleifera molecules and improving the oil quality and other properties by the gene engineering method, as well as building a camellia oleifera genetic system.
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Description

technical field

[0001] The invention belongs to the field of camellia oleifera cell engineering, and in particular relates to a tissue culture method for inducing adventitious buds by using camellia oleifera hypocotyls as explants, and then obtaining complete test-tube seedlings. Background technique

[0002] Camellia oleifera (Camellia oleifera) is an evergreen shrub or small tree of the genus Camellia in the family Theaceae. The unsaturated fatty acid content of camellia oil is more than 90%. It has a good color and fragrance, unique flavor, rich nutrition, storage resistance, lowers blood pressure, blood lipids and softens blood vessels, and plays an important role in the prevention of cardiovascular diseases. It is a high-quality edible oil One of them is known as "Oriental Olive Oil". At the same time, the by-products of camellia oleifera are also high-quality raw materials for the production of fertilizers, pesticides, and tannins. Camellia oleifera is mainly distrib...

Claims

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