Method for obtaining regenerated plant by utilizing camellia oleifera hypocotyl
A technology for regenerating plants and Camellia oleifera, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of transgenic Camellia oleifera without finding explants, unable to apply, and unable to transgenic plants, etc.
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Examples
Embodiment 1
[0037] Do the following in order:
[0038] 1. Select mature raw camellia oleifera seeds of the same year, remove the seed coat, rinse the seeds with tap water for 3-5 minutes, then soak for 10-15 hours, change the water 2-3 times in the middle, and then use 75% of the water in the ultra-clean workbench Soak in alcohol for 20-30s, rinse with sterile water for 3-5 times, and then use 0.1% HgCl 2 Disinfect for 3-5 minutes, rinse with sterile water 5-6 times, cut off most of the endosperm, inoculate the seed embryo with endosperm in WPM basic medium, culture in dark for 2-3 days, then transfer to light for 20- Sterile seedlings can be obtained in 25 days; the culture temperature is 26±1°C, the light intensity is 2100-2200lx, and the light time is 12-14h / d.
[0039] Table 1 Effect of different disinfection treatment methods on the disinfection effect of Camellia oleifera germ
[0040]
[0041] 2. Select the hypocotyls of aseptic seedlings of camellia oleifera, cut them into 1....
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Description
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Application Information
- IPC
- A01H4/00
- Inventors
- 张琳; 李泽