Method for obtaining regenerated plant by utilizing camellia oleifera hypocotyl

A technology for regenerating plants and Camellia oleifera, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of transgenic Camellia oleifera without finding explants, unable to apply, and unable to transgenic plants, etc.

Active Publication Date: 2017-04-05
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many studies on the tissue culture of Camellia oleifera. Zhang Zhijun et al. used the cotyledons of Camellia oleifera clones to obtain regenerated plants through somatic embryogenesis. Li Ze et al. It is of great significance in cultivating rapid propagation, and cannot be applied to the transformation of Camellia oleifera genetic system to obtain transgenic plants
The most commonly used method for the transformation of plant genetic systems is the Agrobacterium-mediated method to infect plant leaves, hypocotyls, petioles, radicles and other organs, while the induction rate of adventitious buds induced by tea leaves is only 17.86%, and it cannot be applied to camellia oleifera at present. In terms of genetic system transformation, the Camellia oleifera transgene has not yet found a suitable explant

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for obtaining regenerated plant by utilizing camellia oleifera hypocotyl
  • Method for obtaining regenerated plant by utilizing camellia oleifera hypocotyl
  • Method for obtaining regenerated plant by utilizing camellia oleifera hypocotyl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Do the following in order:

[0038] 1. Select mature raw camellia oleifera seeds of the same year, remove the seed coat, rinse the seeds with tap water for 3-5 minutes, then soak for 10-15 hours, change the water 2-3 times in the middle, and then use 75% of the water in the ultra-clean workbench Soak in alcohol for 20-30s, rinse with sterile water for 3-5 times, and then use 0.1% HgCl 2 Disinfect for 3-5 minutes, rinse with sterile water 5-6 times, cut off most of the endosperm, inoculate the seed embryo with endosperm in WPM basic medium, culture in dark for 2-3 days, then transfer to light for 20- Sterile seedlings can be obtained in 25 days; the culture temperature is 26±1°C, the light intensity is 2100-2200lx, and the light time is 12-14h / d.

[0039] Table 1 Effect of different disinfection treatment methods on the disinfection effect of Camellia oleifera germ

[0040]

[0041] 2. Select the hypocotyls of aseptic seedlings of camellia oleifera, cut them into 1....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for obtaining regeneration plants by use of camellia oleifera hypocotyl and belongs to the field of plant cell engineering. The method mainly comprises the step that adventive buds are induced by camellia oleifera hypocotyl. Through multiplicative subculture, strong seedling culture, rooting culture, acclimatization and transplant and the like of the adventive buds, not only is a new way provided for quick propagation of favorable camellia oleifera plants, but also a solid foundation is laid for improving the resistivity of camellia oleifera, accelerating the breeding process of camellia oleifera molecules and improving the oil quality and other properties by the gene engineering method, as well as building a camellia oleifera genetic system.

Description

technical field [0001] The invention belongs to the field of camellia oleifera cell engineering, and in particular relates to a tissue culture method for inducing adventitious buds by using camellia oleifera hypocotyls as explants, and then obtaining complete test-tube seedlings. Background technique [0002] Camellia oleifera (Camellia oleifera) is an evergreen shrub or small tree of the genus Camellia in the family Theaceae. The unsaturated fatty acid content of camellia oil is more than 90%. It has a good color and fragrance, unique flavor, rich nutrition, storage resistance, lowers blood pressure, blood lipids and softens blood vessels, and plays an important role in the prevention of cardiovascular diseases. It is a high-quality edible oil One of them is known as "Oriental Olive Oil". At the same time, the by-products of camellia oleifera are also high-quality raw materials for the production of fertilizers, pesticides, and tannins. Camellia oleifera is mainly distrib...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张琳李泽谭晓风林青邹锋曾艳玲龙洪旭赵广
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap