Valeriana Fauriei Briq. tissue culture breeding method

A technology of tissue culture and northern valerian, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of easy scale, fast propagation speed, and stable system

Active Publication Date: 2015-01-28
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the tissue culture of Valeriana plants. There are reports of using cotyledons to induce bud clusters under sterile conditions. At the same time, adventitious roots are only induced from leaves of V. Breeding Research Reports

Method used

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  • Valeriana Fauriei Briq. tissue culture breeding method
  • Valeriana Fauriei Briq. tissue culture breeding method
  • Valeriana Fauriei Briq. tissue culture breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Materials:

[0039] The test material, that is, the tissue culture explant is the young stem section of the northern valerian plant.

[0040] 2. Experimental steps:

[0041] (1) Explant induction: Rinse the tender stem section with tap water for 20 minutes, disinfect it with 70% ethanol for 1 minute, take it out after disinfecting it with 1% sodium hypochlorite solution for 15 minutes, rinse it 4 times with sterile water, and inoculate it at 1.0 mg / L 6-BA, 0.02mg / L IBA cultured on MS medium, the medium was added with 3% sucrose, 0.8% agar, pH 5.8, the culture temperature was 25°C, and the light time was 16h·d -1 , light 3000lux;

[0042](2) Subculture propagation culture: cut off the adventitious buds formed in step (1) under aseptic conditions, and inoculate the MS with a pH of 5.8 in 1.0mg / L6-BA, 0.02mg / L IBA and 8g / L agar. Proliferation experiments were carried out on the proliferation medium to obtain clustered shoots for subculture proliferation. The medium w...

Embodiment 2

[0056] 1. Materials:

[0057] The test material, that is, the tissue culture explant is the young stem section of the northern valerian plant.

[0058] 2. Experimental steps:

[0059] (1) Explant induction: Rinse the tender stems with tap water for 15 minutes, sterilize them with 60% ethanol for 30 seconds, sterilize them with 0.5% sodium hypochlorite solution for 10 minutes, take them out, rinse them three times with sterile water, and inoculate them at 0.5 mg / L 6-BA, 0.01mg / L IBA cultured on MS medium, the medium was added with 2% sucrose, 0.5% agar, pH 5.0, the culture temperature was 20°C, and the light time was 12h·d -1 , light 2500lux;

[0060] (2) Subculture proliferation culture: cut off the adventitious buds formed in step (1) under aseptic conditions, and inoculate in 0.5 mg / L 6-BA, 0.01 mg / L IBA and 8 g / L agar with a pH of 5.8 MS Proliferation experiments were carried out on the proliferation medium to obtain clustered shoots for subculture proliferation. The med...

Embodiment 3

[0074] 1. Materials:

[0075] The test material, that is, the tissue culture explant is the young stem section of the northern valerian plant.

[0076] 2. Experimental steps:

[0077] (1) Explant induction: Rinse the tender stems with tap water for 25 minutes, sterilize them with 80% ethanol for 90 seconds, take them out after sterilizing them with 1.5% sodium hypochlorite solution for 20 minutes, rinse them with sterile water 5 times, and inoculate them at 0.2 mg / L 6-BA, 0.01mg / L IBA cultured on MS medium, the medium was added with 5% sucrose, 2% agar, pH 6, the culture temperature was 30°C, and the light time was 20h·d -1 , light 4000lux;

[0078] (2) Subculture proliferation culture: cut off the adventitious buds formed in step (1) under aseptic conditions, and inoculate the MS with a pH of 5.8 in 0.2mg / L6-BA, 0.01mg / L IBA and 8g / L agar. Proliferation experiments were carried out on the proliferation medium to obtain the clustered shoots of subculture proliferation. The ...

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Abstract

The invention aims to provide a Valeriana Fauriei Briq. tissue culture breeding method. The method is as below: 1, using young stems of Valeriana Fauriei as explants and conducting aseptic treatment; 2, placing sterile Valeriana Fauriei stems on a solid culture medium to induce adventitious buds; 3, inoculating the adventitious buds onto the solid medium for rooting and seedling, induced; and 4, transplanting the tissue culture seedlings to the culture medium for hardening seedling cultivation. The invention is not limited or influenced by natural conditions and, has the characteristics of fastness and easiness for large scale. The invention can ease the problem of shortage of wild Valeriana Fauriei resources and of destruction of ecological balance caused by overexploitation.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture propagation, in particular to a tissue culture propagation method of Valeriana fauriei Briq., a medicinal plant of the genus Valeriana. Background technique [0002] Northern valerian (Valeriana fauriei Briq.) is a wild perennial herbaceous plant belonging to the genus Valerianaceae (Valeriana). Valerian root is flat and pungent in nature, has the effects of calming the nerves, relieving spasm and relieving pain, and the rhizome has been used as a sedative for thousands of years. Northern valerian is the representative of the genus Valeriana, distributed in Northeast China (Heilongjiang, Jilin, Liaoning) and East China (Jiangsu, Anhui, Zhejiang, Jiangxi, Taiwan), and extends westward to Henan, Shaanxi and other places. Northern valerian grows in hillside meadows, forest wetlands, and forest edge roadsides below 2000m above sea level. At present, the pharmacological effects and chemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张鑫宋经元辛天怡陈士林
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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