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A method for inducing adventitious buds by germination of Korean pine accelerated germination seeds

A seed and Korean pine technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of low adventitious bud differentiation rate, Korean pine organ difficulties, etc., and achieve high adventitious bud induction rate, convenient material collection, and easy operation simple effect

Active Publication Date: 2020-01-14
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the current Korean pine organs are difficult to produce and the differentiation rate of adventitious buds is low

Method used

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  • A method for inducing adventitious buds by germination of Korean pine accelerated germination seeds
  • A method for inducing adventitious buds by germination of Korean pine accelerated germination seeds
  • A method for inducing adventitious buds by germination of Korean pine accelerated germination seeds

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specific Embodiment approach 1

[0023] Specific embodiment one: a kind of method of utilizing Korean pine accelerated germination seed to germinate bud body to induce adventitious bud in the present embodiment is specifically realized through the following steps:

[0024] (1) Extraction and disinfection of explants: get the mature Korean pine seeds harvested in the previous year through mixed sand and low-temperature kiln storage for accelerating germination. Wash with washing powder, rinse under running water, remove the seed husk, soak in 10% sodium hypochlorite for 15 minutes, rinse with sterile water for 5 times, soak in 70% alcohol for 1mim, rinse with sterile water for 5 times , and then placed in a 0.1% mercuric chloride solution for disinfection for 10 minutes, rinsed with sterile water for 5 times, to obtain pre-germinated Korean pine seed explants;

[0025] (2) Induction of adventitious buds: the explants obtained by the disinfection treatment in step (1) were cut off the radicle part in the ultra-...

specific Embodiment approach 2

[0030]Specific embodiment two: what this embodiment is different from specific embodiment one is: in the step (1), the mature Korean pine seed is the mature Korean pine seed that gathers through the low-temperature sand storage vernalization process in October of the previous year. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0031] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the explants in the step (1) are drawn and the disinfection treatment method is: get the mature Korean pine seeds that are harvested in the previous year through the low-temperature level sand storage germination process , take the 0.5-1cm explant material that protrudes from the outer seed coat at the radicle portion, first the bud body extracted from the Korean pine is taken out from the seed shell, after removing the endosperm, washing with washing powder water, and rinsing under running water, the mass percentage is Soak in 10% sodium hypochlorite for 20 minutes, rinse 5 times with sterile water, the volume percentage content is 70% alcohol 1mim, rinse 5 times with sterile water, soak in 0.1% mercuric chloride for 15 minutes, rinse 5 times with sterile water, and complete the disinfection treatment .

[0032] Others are the same as in the first embodiment.

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Abstract

The invention discloses a method for inducing adventitious buds by utilizing pinus koraiensis germination-accelerated seed germination buds, and relates to the field of forest seedling propagation. The invention aims to solve the problems that current organogenesis of pinus koraiensis is difficult and a differentiation rate of adventitious buds is low. The method comprises the following steps: (1)taking materials of explants and performing disinfection treatment; (2) performing induction differentiation cultivation on the adventitious buds; (3) performing elongation cultivation on the adventitious buds; and (4) performing rooting induction cultivation on regenerated plants. The method specially comprises the following steps: using germination-accelerated pinus koraiensis seeds as the explants, performing inoculation on a differentiation culture medium by the explants, and performing cultivation for 30 days; and after the adventitious buds are obtained, transferring the adventitious buds to an elongation culture medium, continuing cultivation for 60 days (performing subculture once in the process), when cluster seedlings grow to a length of 2-3 cm, performing cutting, performing separation, transferring the separated seedlings to a rooting medium, and performing domestication. According to the method disclosed by the invention, an induction rate of the adventitious buds reaches92.2%, average quantity of buds per explant is 10-12, and after elongation growth cultivation is performed for 60 days, a height of each bud is 3.5 cm, and the rooting rate reaches 52%.

Description

technical field [0001] The invention relates to the field of propagation of forest tree seedlings, and relates to a method for inducing adventitious buds by utilizing the germination bodies of Korean pine accelerated germination seeds. Background technique [0002] Korean pine (Pinus koraiensis) is a tall tree species of the genus Pinus in the gymnosperm family Pinus. Economic Value. At present, there is a great demand for Korean pine seedlings in the market, but due to the long production cycle of Korean pine, low seed setting rate, and the decline of good traits of offspring, the supply of Korean pine seedlings is in short supply and has become a bottleneck for the development of the Korean pine industry. The in vitro rapid propagation technology of tissue culture can effectively solve the above problems. Carry out research on the in vitro culture of adventitious buds of Korean pine, and explore an effective way to accelerate the propagation speed of Korean pine through t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁艳高美玲陈阳李诗佳赵艳徐洪国梁晓雪孙悦罗枫新赵蕊阳付雨晴
Owner QIQIHAR UNIVERSITY
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