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97results about How to "Short regeneration period" patented technology

Potato isolated culture one-step seedling culture medium and optimization method and seedling method thereof

The invention discloses a method for optimizing a potato isolated culture one-step seedling culture medium. The culture medium makes leaves of a potato test tube plantlet undergo isolated culture to form a seedling in one step. The one-step seedling culture medium takes an MS culture medium as a basal medium, and 6-benzyladenine, naphthyl acetic acid and 2,4-dichlorphenoxyacetic acid with different concentrations and combinations are supplied in 1L of the MS culture medium; adventitious buds and adventitious roots are directly differentiated through callus induction; and the potato regenerated seedling is obtained in one step. The callus obtained by induction in a primary culture medium is unnecessarily inoculated to a differential medium for regenerating the seedling. Compared with an isolated culture multi-step regenerated seedling method, the method of the invention has the advantages of simplified steps, short culture period, high repeatability, and high seedling survival rate.
Owner:SICHUAN AGRI UNIV

Method for preparing and regenerating rhizopus oryzae protoplast for producing L-lactic acid at high yield

InactiveCN102061266AUniform sizeHigh number of preparationsFungiMicroorganism based processesBiotechnologySpore
The invention relates to a method for preparing and regenerating a rhizopus oryzae protoplast for producing L-lactic acid at high yield, comprising the following steps of: A. preparing a rhizopus oryzae spore suspension; B. preparing a seed culture liquid; C. preparing a hypha suspension; D. enzymolyzing a cell wall; E. separating a protoplast; and F. regenerating the protoplast. The rhizopus oryzae protoplast prepared with the method disclosed by the invention is in an approximately-circular shape and has higher activity; the preparation quantity and the regeneration rate of the rhizopus oryzae protoplast can respectively reach 2.74*10<6> / mL and 6.8 percent to the maximum extent. The method is easy to operate, has low requirements for the equipment, and provides a technical reference for preparing and regenerating the rhizopus oryzae protoplast. By using the method disclosed by the invention, the protoplast can be quickly prepared with short regeneration period and high regeneration speed; and the preparation efficiency of the protoplast can be effectively improved. The protoplast prepared with the method provided by the invention completely meets the requirement for fusing the protoplast. The invention provides an operation method with practical significance for preparing and regenerating the protoplast.
Owner:HEFEI UNIV OF TECH

High-temperature condensed water recycling and purifying system with ceramic membrane filter

The invention provides a high-temperature condensed water recycling and purifying with a ceramic membrane filter. The high-temperature condensed water recycling and purifying with the ceramic membrane filter comprises a concentrating tank, a dual-pump structure, the ceramic membrane filter, a cleaning tank and a water purifying tank; the water outlet of the concentrating tank is connected with the water inlet of the dual-pump structure through a pipeline; the water outlet of the dual-pump structure is connected with the circulating water inlet of the ceramic membrane filter; the circulating water outlet of the ceramic membrane filter is connected with a communicating pipe of the dual-pump structure through a pipeline; the water purifying port of the ceramic membrane filter is connected tothe water purifying tank through a pipeline; the water inlet of the cleaning tank is connected with the water purifying port of the ceramic membrane filter through a three-way pipeline; the cleaning solution outlet of the cleaning tank is connected with the water inlet of the dual-pump structure through a three-way pipe; and the cleaning water returning port of the cleaning tank is connected withthe circulating water inlet of the ceramic membrane filter. The high-temperature condensed water recycling and purifying with the ceramic membrane filter is divided into two processing units so that separation and filtration can be separated; and by continuous concentration of water, a processing device for separating according to the physical characteristics of a medium is provided. The device occupies a small area, has high integration degree and a short construction period, and is easy to install.
Owner:北京中耐科技股份有限公司

Method for preparing low-carbon olefin by taking biomass synthetic gas as raw material with dimethyl ether two-step method

The invention provides a method for preparing low-carbon olefin by taking biomass synthetic gas as a raw material with a dimethyl ether two-step method, comprising the following steps of: taking the biomass synthetic gas with a low hydrogen-carbon ratio as the raw material and carrying out methanol synthesis and methanol dehydration reactions on the raw material and a dimethyl ether synthesis catalyst in a one-step fixed bed reactor to prepare an oxygen-containing hydrocarbon with higher dimethyl ether content; after dehydrating a reaction product, raising the temperature to 120 DEG C and introducing the product into a two-step fixed bed reactor to be continuously reacted with a dimethyl ether catalytic conversion catalyst under normal pressure, so as to generate the low-carbon olefin, wherein the dimethyl ether synthetic catalyst is a compound catalyst composed of a Cu / Zn / Al methanol synthesis catalyst and an HZSM-5 (Hydro Zeolite Socony Mobil-5) methanol dehydration catalyst; a carrier of the dimethyl ether catalytic conversion catalyst is an SAPO-34 (Silicoaluminophosphate-34) and HZSM-5 mechanically-mixing molecular sieve; an active component is nickel and a weight loading amount is 0.5-1.5 percent. The CO conversion rate of the method provided by the invention can reach to 39.2-77.4 percent, the selectivity of the low-carbon olefin can reach to 71.5-84.6 percent and the carbon utilization rate of the synthetic gas can reach to 10.2-32.8 percent. The invention provides a new technical route for the production of the low-carbon olefin in our country.
Owner:GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI

Purification process for caprolactam

The invention discloses a purification process for caprolactam. The purification process comprises the following steps: cooling crude caprolactam aqueous liquor obtained in an extracting process to 43-45 DEG C; then, passing the cooled crude caprolactam aqueous liquor through an ion exchange system which is composed of a chromatography resin device and an ion exchange device; and carrying out hydrogenation, evaporation and distillation to prepare a caprolactam finished product. The chromatography resin device is introduced to the ion exchange system, so that the problem that anion exchange resin is easy to pollute and difficult to regenerate is solved. The resin impurity removal and the resin regeneration are realized by continuously moving the bed-layer chromatography resin device and bed ion exchange resin, the regeneration period of the resin is shortened, and the stability of the discharge purity of the caprolactam aqueous liquor in an ion exchange process is guaranteed. Moreover, the number of resin columns is increased and the size of the resin columns is reduced, so that the total filling amount in the continuous ion exchange system is reduced, and the dosage of acid and alkaline in the regenerating process is also correspondingly reduced, and thus, the cost is saved and the secondary pollution is lowered.
Owner:河北美邦工程科技股份有限公司 +1

Catalyst for dehydrogenation of light alkane and preparation method of catalyst

The invention belongs to the field of petroleum refining and chemical engineering, and relates to a catalyst for dehydrogenation of light alkane and a preparation method of the catalyst. The light alkane can be dehydrogenated to produce corresponding light olefin, aluminum oxide is used as a carrier of the catalyst, active components are platinum and tin, and auxiliary active components are potassium, cerium and lanthanum. Platinum accounts for 0.2-0.6wt% of the carrier, tin accounts for 0.3-1wt% of the carrier, potassium accounts for 0.05-0.5wt% of the carrier, cerium accounts for 0.05-1.0wt% of the carrier, and lanthanum accounts for 0.01-1.0wt% of the carrier. When the catalyst is used, the carbon deposit of the catalyst can be reduced, the catalyst selectivity and conversion rate of the catalyst are improved, and the service life and activity cycle of the catalyst are prolonged.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)

Rapid propagation method of Longya lilies

The invention relates to a rapid propagation method of Longya lilies. The method comprises steps as follows: a seed ball of the Longya lilies is taken, a tissue block with an adventitious bud cluster is obtained after inducted growth culture of adventitious buds, and Longya lily seedlings are obtained after induced rooting culture. The rapid propagation method has the benefits as follows: the regeneration efficiency of the method is increased, the bud ratio reaches about 100%, the pollution rate is reduced, the repeated pollution situation cannot occur in the culture process, the induction rate can be increased and the regeneration cycle can be shortened by adjusting culture medium conditions, and the quality and the yield of the Longya lilies are effectively improved.
Owner:韦丽

Method and equipment for treating opium poppy

The invention discloses a method for treating opium poppy extract, which comprises the following steps: a, adding a polar solvent into the opium poppy extract, and reacting to obtain a first treating fluid; b, adding a filter aid into the first treating fluid, and filtering to obtain a first extract liquid; c, separating the first extract liquid through a chromatographic column which is filled with spherical silica gel of a bonded stationary phase and a mixture of alcohol and inorganic acid serving as a mobile phase; and d, eluting to obtain a fraction containing alkaloid. Compared with the prior art, the method has the advantages of separating morphine, codeine, thebaine and other monomer alkaloids, and the alkaloid monomer has high purity and large yield.
Owner:李显林

Production method for 1-butylene

The present invention relates to a preparation method for butylene-1; the method is to overcome the shortcoming in the prior technology that: the selectivity of the produced butylene-1 is lower; the benefit ratio is lower; the regeneration period for the catalyzer is much shorter; and the energy consumption is much higher. The steps in turn include: (1) the raw material including butylenes is contacted with a molecule screen catalyzer to produce the first reaction flow with the molar ratio between butylene-2 and butylene-1 closing to the thermodynamics balance value; (b) the first reaction flow enters a separated tower for separating to obtain butylene-1 in the tower top and the second reaction flow including butylene-2 is obtained in the tower bottom; (c) the second reaction flow used as butylene-2 directly or turned back to the difference reaction device to be reacted continually; the method can solve the problem greatly, which can be uses in the industrial production of butylene-1.
Owner:CHINA PETROLEUM & CHEM CORP +1

A diesel soot particulate filter cartridge

The invention relates to diesel soot particulate filter cartridge having a central filter cartridge axis. The filter cartridge has a radial flow. The filter cartridge comprises a porous structure. The porous structure is coiled around the central filter cartridge axis. The porous structure comprises non-sintered metal fibers, said fibers having a roughness being higher than 1.7. The invention further relates to a method of manufacturing a diesel soot particulate filter cartridge. Furthermore the invention relates to a multicartridge diesel soot particulate filter. The flow through the multicartridge diesel soot particulate filter is axial while the flow through the filter cartridges is radial.
Owner:NV BEKAERT SA

Regeneration method of waste activated carbon and application

The invention provides a regeneration method of waste activated carbon and application. The regeneration method of the waste activated carbon comprises the following steps of conducting primary vacuumpyrolysis on the waste activated carbon to obtain first treated activated carbon; mixing the first treated activated carbon with a regeneration solvent, and conducting vibration treatment after solid-liquid separation to obtain second treated activated carbon; conducting insulation treatment on the second treated activated carbon, and conducting secondary vacuum pyrolysis to obtain regenerated activated carbon. According to the application of the regeneration method of the waste activated carbon, the regeneration method is used for regeneration of the waste powdered activated carbon in the decoloring stage of medicine production. According to the regeneration method of the waste activated carbon, the regeneration efficiency is high, and the period is short. In the regeneration process, noacid-base waste liquor is generated, and the regeneration solvent can be recycled multiple times after being distilled and recycled. The adsorption capability and the specific area of the activated carbon are restored, and the index of the regenerated activated carbon reaches or even exceeds that of new activated carbon.
Owner:BEIFANG UNIV OF NATITIES

Chelate resin tower regeneration prolongation process method

The present invention discloses a chelate resin tower regeneration prolongation process method comprising the following steps: step 1, first crude saline is added with high purity phosphoric acid for precipitation processing; step 2, the processed first crude saline is filtered; and step 3, the filtrate after filtering in the step2 is introduced into a chelate resin tower for adsorption. The chelate resin tower regeneration prolongation process method well solves the problem of frequent regeneration of the chelate resin tower, greatly extends the resin tower regeneration cycle, reduces labor intensity, saves material and power required for regeneration, reduces waste water efflux, and reduces the amount of wastewater reused in the saline process to achieve the increase in the amount of brine dosage.
Owner:WUHU RONGHUI CHEM IND

Online adsorbent regeneration method for SF6 purification treatment device

An online adsorbent regeneration method for an SF6 purification treatment device includes the following steps: cutting out a nitrogen inlet pipeline, an SF6 inlet pipeline, a first pipeline, a secondpipeline, a third pipeline, a fourth pipeline, a fifth pipeline, a sixth pipeline and a seventh pipeline; connecting all the pipelines, a heating unit and an adsorption tower as required; mounting valves, a pressure reducing valve, a filter and a vacuum pump on the corresponding pipelines; and performing normal purification treatment, and discharging the obtained purified gas to the outside through a lye tank tail gas outlet. When the adsorption capability of an adsorbent decreases or the processing capacity is lost, inert nitrogen goes through the adsorption tower after being heated in orderto uniformly heat the adsorbent in the adsorption tower, and then the adsorption tower is vacuumized to realize online depressurization and heating regeneration, so uniform distribution of the temperature in the cavity of the adsorption tower is ensured, a heating tube and the sorbent is completely isolated, inadvertent operation of workers, untimely protection of a temperature control system andother hidden dangers are avoided, the problem of airtightness of the device due to loading and unloading is completely solved, the regeneration cycle of the adsorbent is greatly improved, and the recycling of the adsorbent is realized.
Owner:ELECTRIC POWER RES INST OF STATE GRID ANHUI ELECTRIC POWER +2

High-efficiency genetic transformation method using soybean Jilin No.35 embryonic tip as explant

The invention relates to a high-efficiency genetic transformation method using a soybean Jilin No.35 embryonic tip as an explant and belongs to the technical field of plant transgenosis. The method comprises the following steps of: selecting Jilin No.35 soybean seeds with integrated seed coat and without diseases; performing ultrasonic treatment on the explant; transferring the infected embryonic tip explant into a co-culture medium; co-cultivating; inoculating into a rooting medium; and transplanting to a nutrition pot after the root grows strong. According to the method, the regeneration cycle is shortened. Through the adoption of a strong and effect infection method, browning degree and necrosis degree during co-cultivation are reduced, and conversion rate is increased.
Owner:JILIN UNIV

Regeneration culture method of whole cotyledonary joint of hyacinth bean

InactiveCN102057872AHigh number of adventitious budsCapped Bud LessPlant tissue cultureHorticulture methodsRadicleGrowth plant
The invention relates to a regeneration culture method of the whole cotyledonary joint of a hyacinth bean, belonging to the field of modern biotechnology. The method is characterized by comprising the following steps of: (1) sterilizing, soaking and inoculating a hyacinth bean seed to 0-0.5 mg / l of MSB5+6-BA as a germination culture medium to be cultured and germinating an aseptic seedling; (2) removing original leaves, an upper hypocotyl and a radicle from the aseptic seedling, keeping two cotyledons and 3cm of lower hypocotyl, making a micro wound at the joint of the two cotyledons and transferring to 0.6-2.0 mg / l of MSB5+6-BA and 0.2 mg / l of IBA as an adventitious bud inducement culture medium to be induced and germinating; (3) transferring an explant obtained in the step (2) to the adventitious bud inducement culture medium to lengthen; and (4) transferring to 0.1-0.2 mg / l of MSB5+IBA as a rooting culture medium to root and then transplanting to obtain a regeneration plant. The invention has the advantages of large generated adventitious bud quantity, less capping buds, high bud growth speed, short regeneration period, vigorous regeneration plant growth, less tissue culture difference among various seeds and good repetitiveness. The invention is suitable for the tissue culture of various hyacinth bean varieties.
Owner:上海市浦东新区农业技术推广中心

In-vitro rapid propagation method for lycoris radiata

The invention relates to an in-vitro rapid propagation method for lycoris radiata. The method comprises the steps of: taking a bulb of lycoris radiata which is planted in an open field, disinfecting the bulb, and taking down epiphytic cormels near a mother bulb and planting the cormels for 1-2 months; after the bulb is disinfected, removing the outer skin, peeling seed buds, and performing inductive growth culture of adventitious buds to obtain a tissue block with adventitious buds; making the obtained tissue block into a longitudinal tissue block, and performing bud elongation culture on the longitudinal tissue block in a bud elongation culture medium; and when the obtained adventitious buds elongate and generate side buds, cutting the adventitious buds and putting the adventitious buds in a rooting culture medium for inductive rooting culture to obtain lycoris radiata seedlings. The young and tender buds of lycoris radiata are used as explants which directly generate a lot of adventitious buds so as to further obtain regenerated seedlings, so that the regenerating efficiency is increased, the sprouting ratio is not less than 90%, the contamination rate is low, the situation of repeated contamination in a culture process is avoided, and the induction rate can be increased and the regenerating period can be shortened by adjusting the conditions of the culture medium.
Owner:韦丽

High effective tissue culture regeneration of cucumber, and method for transplanting of said seedlings with high viability

InactiveCN101002540AReduce the impact of regenerative abilityHigh regeneration frequencySeed and root treatmentHorticulture methodsBudPlantlet
A method for the tissue culture and transplanting of cucumber includes such steps as taking the aseptic seedling of cucumber, choosing and inoculating explant, inducing regeneration bud, differentiating, growing, rooting culture, pratisizing seedlings and transplanting.
Owner:VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI

Novel capacitive deionization treatment device

The invention relates to a novel capacitive deionization treatment device. A spraying device is arranged at the top of a device body, and four pieces of organic glass are arranged at the side edge part. A water inlet is formed in the lower left side of the side edge part, and a fresh water outlet is formed in the upper right side of the side edge part. The left organic glass, a sealing washer anda negative plate are fixed through studs. The right organic glass, a sealing washer and a positive plate are fixed through studs. A first channel, a second channel... an nth channel are formed in thedevice body at intervals through stainless steel base plates, and a Z-shaped circulation channel is formed among the first channel, the second channel... the nth channel. The stainless steel base plates and the organic glass on the side edges are fixed through studs, two same-stage electrode plates are arranged on the two sides of the same stainless steel base plate, the two same-stage electrode plates are negative plates or positive plates, the number of the negative plates and the number of the positive plates are both n, and the negative plates and the positive plates are arranged in the device body in parallel in a staggered mode.
Owner:JIANGSU JINGYUAN ENVIRONMENTAL PROTECTION

Water treatment control valve

The invention relates to the technical field of water treatment systems, in particular to a water treatment control valve. The water treatment control valve comprises a valve body, an ejector, a water inlet valve spool, a water outlet valve spool and a saline water control valve. The valve body is provided with a water inlet, a water outlet, a water drainage outlet and a salt absorbing branch passageway. The valve body is provided with through holes communicated with an upper part and a lower part of a filter element and an inlet of the ejector. An outlet of the ejector is communicated with the upper part or the lower part of the filter element, the salt absorbing branch passageway is provided with a saline water inlet, and the saline water control valve is arranged at the saline water inlet. A fixed valve block of the water inlet valve spool is provided with three through holes distributed in trisection according to the cross section of the fixed valve block, and a movable valve block is matched with the fixed valve block and provided with a through hole normally communicated with the water inlet. A fixed valve block of the water outlet valve spool is provided with four through holes distributed in quartering according to the cross section of the fixed valve block, and a movable valve block is matched with the fixed valve block and provided with an arc-shaped through blind hole. By different cooperation of the water inlet valve spool, the water outlet valve spool and the saline water control valve, various water treatment functions can be realized, so that a softening valve with water yield exceeding 20 m3 / h can be manufactured.
Owner:WENZHOU RUNXIN MACHINERY MFG

Method for preparing olefins from synthesis gas

The invention relates to a method for preparing olefins from a synthesis gas, and mainly solves the problems that in the process of producing of the olefins in the existing coal chemical industry technology the catalyst is fast in coking, short in regeneration period and low in olefin yield. The method comprises the following steps: a synthesis gas material flow is reacted in a reaction zone to obtain a product material flow; the product material flow is separated into a material flow I which mainly contain carbon dioxide and a decarburized material flow through a decarbonization zone; and thedecarburized material flow is separated into a material flow ii which mainly contains carbon monoxide and hydrogen and a mixed hydrocarbon material flow through a dehydrogenation zone; the carbon dioxide-containing material flow i is at least partially recycled back to the reaction zone; and the carbon monoxide and hydrogen containing flow ii is at least partially recycled back to the reaction zone; according to the method, the problems are well solved, and the method can be used in the production of low-carbon olefin products, and has the advantage of low catalyst coking, long regeneration period, and high olefin yield.
Owner:CHINA PETROLEUM & CHEM CORP +1

Regeneration method of inactivated COS hydrolytic agent

The invention relates to the technical field of regeneration of deactivated catalysts, in particular to a regeneration method of a deactivated COS hydrolytic agent. The regeneration method of the inactivated COS hydrolytic agent comprises the following steps: under the atmosphere of reducing gas, carrying out primary heating and heat preservation on the inactivated COS hydrolytic agent to obtain afirst regenerated COS hydrolytic agent; and maintaining the atmosphere of the reducing gas, carrying out secondary heating on the first regenerated COS hydrolytic agent, introducing carbon dioxide gas containing saturated steam into the atmosphere of the reducing gas, and carrying out heat preservation at a secondary heating temperature to obtain the regenerated COS hydrolytic agent. The regeneration method of the inactivated COS hydrolytic agent provided by the invention is a green regeneration method which does not need to be washed by a solution, does not add an active component additive and can effectively save the regeneration period.
Owner:中琉科技有限公司

Methanol-to-gasoline combined technology

The invention relates to a methanol-to-gasoline combined production technology, and belongs to an important link of a coal-preparing high-clean energy technology. The technology includes the steps: methanol is heated, then a great part of the methanol enters a dimethyl ether reactor, is subjected to crude dehydration, then enters a gasoline transformation reactor and is further dehydrated, and the product passes through a heat exchanger and then is subjected to oil-water separation; crude gasoline is separated to obtain dry gas through a deethanizer; a part of the dry gas is subjected to heat exchange through the heat exchanger, then enters a circulation compressor and together with a dimethyl ether reactor outlet product enters a conversion reactor, so as to achieve the temperature dropping and control purposes; the gasoline having the dry gas removed enters a debutanizer, and removed liquefied gas and the other part of methanol steam undergo a superimposed etherification reaction; crude gasoline coming out of the debutanizer is separated to obtain the product gasoline and heavy oil by a gasoline separation tower; the heavy oil and the other part of the dry gas coming out of the deethanizer are together modified by a transalkylation catalyst to obtain a gasoline component, dry gas and liquefied gas.
Owner:WUHAN KELIN FINE CHEM

Method for in vitro regeneration culture of true leaves of Chinese cabbage

InactiveCN102144561AOptimizing the True Leaf Regeneration System in VitroThe cultivation method is simpleHorticulture methodsPlant tissue cultureBiotechnologyGermplasm
The invention discloses a method for the in vitro regeneration culture of true leaves of Chinese cabbage. In the method, a true leaf is adopted as an explant for the first time on the basis of the traditional tissue culture of the Chinese cabbage, and an in vitro regeneration system of the true leaves of the Chinese cabbage is optimized in terms of appropriate seedling state, cutting mode, inoculation mode and hormone concentration. In the culture method, 6-benzylamino adenine (BA) and naphthylacetic acid (NAA) are only needed to be added into the conventional murashige and skoog (MS) basic culture medium; the method is simple and easy to operate; meanwhile, cultured adventitious buds are large in number, good in quality, robust and not abnormal, and have high regeneration rate and short regeneration period. The invention provides a novel way for obtaining a novel transgenic receptor material of the Chinese cabbage, and has important realistic significance for innovating the germplasm and novel variety of the Chinese cabbage by utilizing a modern genetic engineering technology.
Owner:NORTHWEST A & F UNIV

Method for manufacturing recycled paper by adding loofah sponge fiber

Provided is a method for manufacturing recycled paper by adding loofah sponge fiber. The method comprises the steps that loofah sponge undergoes dry-smashing treatment; degumming treatment is performed; paper products are recovered, and coarse cutting, shake-screening, centrifugal classification and fine cutting treatment is performed; classified recovered paper chippings are soaked, stirred, filtered and stirred to produce pulp; degummed loofah sponge fiber chippings and the recovered paper pulp material are mixed and stirred according to the ratio of 1:9 to 1:1; a dye, a dye-fixing agent and starch are added or visible loofah sponge fiber chippings are supplemented for mixing and stirring treatment; pulp taking for papermaking, press mold water absorption, hot-air constant-temperature drying, paper taking through scrapers and press-polishing and paper rolling are performed to obtain a dyed loofah sponge recycled paper finished product. The fiber content of the recycled paper is supplemented by adding the loofah sponge fiber, and the adsorption and coloring properties of the recycled paper can be improved while the paper is flexible. In addition, in the mode of classified pulping of the recycled paper, the high-quality recycled paper output rate can be greatly improved, added economic benefits can be brought to enterprises by combining with natural texture of the loofah sponge, and meanwhile the method has the special significance on environmental protection.
Owner:湛江市原佳彩印包装材料有限公司

Method for obtaining hedychium coronarium tetraploid plants by slice culture technology

The invention discloses a method for obtaining hedychium coronarium tetraploid plants by a slice culture technology. The method comprises the following steps: cutting a slice from a tube seedling stem section, processing the slice by using colchicines, and then carrying out adventitious bud induction on the processed slice by adopting the slice culture technology to obtain regenerated plants; detecting the regenerated plants; and screening to obtain the tetraploid plants. According to the method, when induced mutation is carried out, the used explants are the slices, only are 0.3-0.5mm in thickness, and are few in cells; when treatment is carried out by using colchicine, the cells of the explants can be fully exposed in a liquid medicine; most of the obtained plants are homozygote; the screening processes of polyploid plants are simplified; and the used chromosome number analysis technology has the advantages of being simple in operation, short in required time, clear in obtained chromosome image and the like. The method disclosed by the invention has the advantages of being simple in operation, high in plant regeneration frequency, short in regeneration cycle, easily available in materials, strong in operability and easy to popularize and promote.
Owner:GUANGDONG UNIV OF EDUCATION

Method for fast reproducing pinellia ternata

The invention relates to a method for fast reproducing pinellia ternate. The method comprises the following steps that a pinellia ternate seedball is taken, inducing growing culturing is carried out on an adventitious bud, a tissue block with adventitious bud clusters is obtained, and root induction culturing is carried out to obtain a pinellia ternate seedling. The method is high in regeneration efficiency, the bud ratio is about 100%, the pollution rate is lowered, repeated pollution is avoided in the culturing process, and inductivity can be improved and the regeneration period can be shortened by adjusting culture medium conditions.
Owner:韦丽

Method for directly obtaining plants through eggplant anther culture and medium

ActiveCN106508673AThere is no differentiation problemFew stepsHorticulture methodsPlant tissue cultureCallus formationPloidy
The invention provides a method for directly obtaining plant through eggplant anther cultivation as well as a medium thereof, which belong to the technical field of plant tissue culture technique. The method comprises the following steps: 1) selecting fresh buds; 2) disinfecting the surface of bud and acquiring the aseptic anther; 3) inoculating the aseptic anther in the late uninucleate stage in an anther culture solid medium, culturing the aseptic anther for 2-8 days at dark environment at the temperature of 33-37 DEG C, and performing illumination culture for 30-40 days at the temperature of 25-28 DEG C; and 4) transferring the cotyledonous embryoid to a healthy seedling solid medium for continuously culturing the materials, and growing to obtain the plant. The method has the following advantages that the method is simple and easy to carry out, the in-vitro culture of the anther can directly induce the embryoid to obtain the haploid plant, a condition that the callus formed by anther culture induction is transferred to a differentiation medium, and then the plant regeneration is induced can be avoided; and the chromosome ploidy mixing phenomenon due to participation of diploid somatic cells such as the anther wall in callus can be avoided.
Owner:北京市海淀区植物组织培养技术实验室 +1

One-step plantlet formation medium and method for isolated culture of lamiophlomis rotata

The invention discloses a one-step plantlet formation medium and method for the isolated culture of lamiophlomis rotata. By adopting the one-step plantlet formation medium, a lamiophlomis rotata test-tube plantlet leaf forms a plantlet in one step through isolated culture. The one-step plantlet formation culture medium is characterized in that a MS (Murashige and Skoog) medium is used as a basic medium, and 6-benzylaminopurine (6-BA), naphthylacetic acid (NAA) and 2,4-dichlorphenoxyacetic acid (2,4-D) which are different in concentrations and combinations are added to the base medium. According to the one-step plantlet formation method, the test-tube plantlet leaf serving as an explant is inoculated, then is induced by a callus and is directly differentiated into an adventitious bud and an adventitious root, so that the lamiophlomis rotata regenerated plantlet is obtained by one step. By adopting the one-step plantlet formation method, the callus obtained by induction in an initial medium does not need to be transferred into a differential medium for regenerating the plantlet. Compared with an isolated culture multi-step regeneration plantlet formation method, the one-step plantlet formation method has the advantages that the steps are simplified, the culture period is short, the repeatability is good, and the planting percent is high.
Owner:SICHUAN AGRI UNIV

Environmental-friendly desulfurization process of smoke

The invention discloses an environmental-friendly desulfurization process of smoke. The desulfurization process comprises the steps of (1) dust elimination and cleaning of smoke: making smoke pass through a dedusting channel to eliminate a part of dust, and adding a scrubbing solution into the channel, wherein the scrubbing solution is prepared from, by weight, 40-50 parts of hydrogen peroxide, 5-10 parts of sodium hydroxide particles, 1-5 parts of dodecyl dimethyl betaine, and 1-3 parts of sodium carboxymethylcellulose; (2) primary removal of hydrogen sulfide: adopting iron oxide desulfurizer under a normal temperature in the primary removal of hydrogen sulfide; (3) first-level fine desulfurization: adopting active carbon desulfurizer under a normal temperature in the first-level fine desulfurization; (4) second-level fine desulfurization: adopting molecular sieve desulfurizer in the second-level fine desulfurization. The multiple types of organic sulfur adsorbent adopted in the environmental-friendly desulfurization process of smoke are all reproducible and capable of being recycled, and the environmental-friendly desulfurization process of smoke has the advantages of getting rid of interference conducted on adsorption and desorption of absorbent by inorganic sulfur impurities, improving efficiency of desulfurization, saving energy to protect environment, decreasing consumption, and increasing the purity and cleanliness of smoke after desulfurization.
Owner:HEFEI DAZHUO ELECTRIC POWER CO LTD

One-step seedling emergence and quick propagation method for tissue culture of sedum aizoon

The invention relates to a one-step seedling emergence and quick propagation method for the tissue culture of sedum aizoon, and belongs to the technical field of plant tissue culture. Through the adoption of the method disclosed by the invention, the problems that the coefficient of a conventional propagation method for the sedum aizoon is low, the growth is slow, and the large-scale production is difficult to form are solved. The method comprises the following steps: (1) performing sterilization and disinfection; (2) performing one-step seedling emergence culture; (3) domesticating and transplanting test-tube plantlets, wherein rooting seedlings are taken out and are put in a seedling exercising room with enough illumination, after 3-5 days, bottle mouths are opened, seedlings are continuously exercised for 2-3 days, then the test-tube plantlets are taken out, root culture mediums are cleaned and are transplanted in substrates consisting of peat soil and vermiculite, the ratio of the weight parts of the peat soil to the weight parts of the vermiculite is 1: 1, and the condition of spraying small seedlings 2-3 times a day for first 7 days is ensured. The method disclosed by the invention is used for the tissue culture of the sedum aizoon.
Owner:RIZHAO POLYTECHNIC
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