96results about How to "Short regeneration period" patented technology

The invention relates to a method for preparing and regenerating a rhizopus oryzae protoplast for producing L-lactic acid at high yield, comprising the following steps of: A. preparing a rhizopus oryzae spore suspension; B. preparing a seed culture liquid; C. preparing a hypha suspension; D. enzymolyzing a cell wall; E. separating a protoplast; and F. regenerating the protoplast. The rhizopus oryzae protoplast prepared with the method disclosed by the invention is in an approximately-circular shape and has higher activity; the preparation quantity and the regeneration rate of the rhizopus oryzae protoplast can respectively reach 2.74*10<6>/mL and 6.8 percent to the maximum extent. The method is easy to operate, has low requirements for the equipment, and provides a technical reference for preparing and regenerating the rhizopus oryzae protoplast. By using the method disclosed by the invention, the protoplast can be quickly prepared with short regeneration period and high regeneration speed; and the preparation efficiency of the protoplast can be effectively improved. The protoplast prepared with the method provided by the invention completely meets the requirement for fusing the protoplast. The invention provides an operation method with practical significance for preparing and regenerating the protoplast.

The invention provides a method for preparing low-carbon olefin by taking biomass synthetic gas as a raw material with a dimethyl ether two-step method, comprising the following steps of: taking the biomass synthetic gas with a low hydrogen-carbon ratio as the raw material and carrying out methanol synthesis and methanol dehydration reactions on the raw material and a dimethyl ether synthesis catalyst in a one-step fixed bed reactor to prepare an oxygen-containing hydrocarbon with higher dimethyl ether content; after dehydrating a reaction product, raising the temperature to 120 DEG C and introducing the product into a two-step fixed bed reactor to be continuously reacted with a dimethyl ether catalytic conversion catalyst under normal pressure, so as to generate the low-carbon olefin, wherein the dimethyl ether synthetic catalyst is a compound catalyst composed of a Cu/Zn/Al methanol synthesis catalyst and an HZSM-5 (Hydro Zeolite Socony Mobil-5) methanol dehydration catalyst; a carrier of the dimethyl ether catalytic conversion catalyst is an SAPO-34 (Silicoaluminophosphate-34) and HZSM-5 mechanically-mixing molecular sieve; an active component is nickel and a weight loading amount is 0.5-1.5 percent. The CO conversion rate of the method provided by the invention can reach to 39.2-77.4 percent, the selectivity of the low-carbon olefin can reach to 71.5-84.6 percent and the carbon utilization rate of the synthetic gas can reach to 10.2-32.8 percent. The invention provides a new technical route for the production of the low-carbon olefin in our country.

The invention belongs to the field of petroleum refining and chemical engineering, and relates to a catalyst for dehydrogenation of light alkane and a preparation method of the catalyst. The light alkane can be dehydrogenated to produce corresponding light olefin, aluminum oxide is used as a carrier of the catalyst, active components are platinum and tin, and auxiliary active components are potassium, cerium and lanthanum. Platinum accounts for 0.2-0.6wt% of the carrier, tin accounts for 0.3-1wt% of the carrier, potassium accounts for 0.05-0.5wt% of the carrier, cerium accounts for 0.05-1.0wt% of the carrier, and lanthanum accounts for 0.01-1.0wt% of the carrier. When the catalyst is used, the carbon deposit of the catalyst can be reduced, the catalyst selectivity and conversion rate of the catalyst are improved, and the service life and activity cycle of the catalyst are prolonged.

The invention relates to a rapid propagation method of Longya lilies. The method comprises steps as follows: a seed ball of the Longya lilies is taken, a tissue block with an adventitious bud cluster is obtained after inducted growth culture of adventitious buds, and Longya lily seedlings are obtained after induced rooting culture. The rapid propagation method has the benefits as follows: the regeneration efficiency of the method is increased, the bud ratio reaches about 100%, the pollution rate is reduced, the repeated pollution situation cannot occur in the culture process, the induction rate can be increased and the regeneration cycle can be shortened by adjusting culture medium conditions, and the quality and the yield of the Longya lilies are effectively improved.

The present invention relates to a preparation method for butylene-1; the method is to overcome the shortcoming in the prior technology that: the selectivity of the produced butylene-1 is lower; the benefit ratio is lower; the regeneration period for the catalyzer is much shorter; and the energy consumption is much higher. The steps in turn include: (1) the raw material including butylenes is contacted with a molecule screen catalyzer to produce the first reaction flow with the molar ratio between butylene-2 and butylene-1 closing to the thermodynamics balance value; (b) the first reaction flow enters a separated tower for separating to obtain butylene-1 in the tower top and the second reaction flow including butylene-2 is obtained in the tower bottom; (c) the second reaction flow used as butylene-2 directly or turned back to the difference reaction device to be reacted continually; the method can solve the problem greatly, which can be uses in the industrial production of butylene-1.

The invention provides a regeneration method of waste activated carbon and application. The regeneration method of the waste activated carbon comprises the following steps of conducting primary vacuumpyrolysis on the waste activated carbon to obtain first treated activated carbon; mixing the first treated activated carbon with a regeneration solvent, and conducting vibration treatment after solid-liquid separation to obtain second treated activated carbon; conducting insulation treatment on the second treated activated carbon, and conducting secondary vacuum pyrolysis to obtain regenerated activated carbon. According to the application of the regeneration method of the waste activated carbon, the regeneration method is used for regeneration of the waste powdered activated carbon in the decoloring stage of medicine production. According to the regeneration method of the waste activated carbon, the regeneration efficiency is high, and the period is short. In the regeneration process, noacid-base waste liquor is generated, and the regeneration solvent can be recycled multiple times after being distilled and recycled. The adsorption capability and the specific area of the activated carbon are restored, and the index of the regenerated activated carbon reaches or even exceeds that of new activated carbon.

An online adsorbent regeneration method for an SF6 purification treatment device includes the following steps: cutting out a nitrogen inlet pipeline, an SF6 inlet pipeline, a first pipeline, a secondpipeline, a third pipeline, a fourth pipeline, a fifth pipeline, a sixth pipeline and a seventh pipeline; connecting all the pipelines, a heating unit and an adsorption tower as required; mounting valves, a pressure reducing valve, a filter and a vacuum pump on the corresponding pipelines; and performing normal purification treatment, and discharging the obtained purified gas to the outside through a lye tank tail gas outlet. When the adsorption capability of an adsorbent decreases or the processing capacity is lost, inert nitrogen goes through the adsorption tower after being heated in orderto uniformly heat the adsorbent in the adsorption tower, and then the adsorption tower is vacuumized to realize online depressurization and heating regeneration, so uniform distribution of the temperature in the cavity of the adsorption tower is ensured, a heating tube and the sorbent is completely isolated, inadvertent operation of workers, untimely protection of a temperature control system andother hidden dangers are avoided, the problem of airtightness of the device due to loading and unloading is completely solved, the regeneration cycle of the adsorbent is greatly improved, and the recycling of the adsorbent is realized.

A method for the tissue culture and transplanting of cucumber includes such steps as taking the aseptic seedling of cucumber, choosing and inoculating explant, inducing regeneration bud, differentiating, growing, rooting culture, pratisizing seedlings and transplanting.

The invention relates to a novel capacitive deionization treatment device. A spraying device is arranged at the top of a device body, and four pieces of organic glass are arranged at the side edge part. A water inlet is formed in the lower left side of the side edge part, and a fresh water outlet is formed in the upper right side of the side edge part. The left organic glass, a sealing washer anda negative plate are fixed through studs. The right organic glass, a sealing washer and a positive plate are fixed through studs. A first channel, a second channel... an nth channel are formed in thedevice body at intervals through stainless steel base plates, and a Z-shaped circulation channel is formed among the first channel, the second channel... the nth channel. The stainless steel base plates and the organic glass on the side edges are fixed through studs, two same-stage electrode plates are arranged on the two sides of the same stainless steel base plate, the two same-stage electrode plates are negative plates or positive plates, the number of the negative plates and the number of the positive plates are both n, and the negative plates and the positive plates are arranged in the device body in parallel in a staggered mode.

The invention relates to a method for preparing olefins from a synthesis gas, and mainly solves the problems that in the process of producing of the olefins in the existing coal chemical industry technology the catalyst is fast in coking, short in regeneration period and low in olefin yield. The method comprises the following steps: a synthesis gas material flow is reacted in a reaction zone to obtain a product material flow; the product material flow is separated into a material flow I which mainly contain carbon dioxide and a decarburized material flow through a decarbonization zone; and thedecarburized material flow is separated into a material flow ii which mainly contains carbon monoxide and hydrogen and a mixed hydrocarbon material flow through a dehydrogenation zone; the carbon dioxide-containing material flow i is at least partially recycled back to the reaction zone; and the carbon monoxide and hydrogen containing flow ii is at least partially recycled back to the reaction zone; according to the method, the problems are well solved, and the method can be used in the production of low-carbon olefin products, and has the advantage of low catalyst coking, long regeneration period, and high olefin yield.

The invention relates to a methanol-to-gasoline combined production technology, and belongs to an important link of a coal-preparing high-clean energy technology. The technology includes the steps: methanol is heated, then a great part of the methanol enters a dimethyl ether reactor, is subjected to crude dehydration, then enters a gasoline transformation reactor and is further dehydrated, and the product passes through a heat exchanger and then is subjected to oil-water separation; crude gasoline is separated to obtain dry gas through a deethanizer; a part of the dry gas is subjected to heat exchange through the heat exchanger, then enters a circulation compressor and together with a dimethyl ether reactor outlet product enters a conversion reactor, so as to achieve the temperature dropping and control purposes; the gasoline having the dry gas removed enters a debutanizer, and removed liquefied gas and the other part of methanol steam undergo a superimposed etherification reaction; crude gasoline coming out of the debutanizer is separated to obtain the product gasoline and heavy oil by a gasoline separation tower; the heavy oil and the other part of the dry gas coming out of the deethanizer are together modified by a transalkylation catalyst to obtain a gasoline component, dry gas and liquefied gas.

Provided is a method for manufacturing recycled paper by adding loofah sponge fiber. The method comprises the steps that loofah sponge undergoes dry-smashing treatment; degumming treatment is performed; paper products are recovered, and coarse cutting, shake-screening, centrifugal classification and fine cutting treatment is performed; classified recovered paper chippings are soaked, stirred, filtered and stirred to produce pulp; degummed loofah sponge fiber chippings and the recovered paper pulp material are mixed and stirred according to the ratio of 1:9 to 1:1; a dye, a dye-fixing agent and starch are added or visible loofah sponge fiber chippings are supplemented for mixing and stirring treatment; pulp taking for papermaking, press mold water absorption, hot-air constant-temperature drying, paper taking through scrapers and press-polishing and paper rolling are performed to obtain a dyed loofah sponge recycled paper finished product. The fiber content of the recycled paper is supplemented by adding the loofah sponge fiber, and the adsorption and coloring properties of the recycled paper can be improved while the paper is flexible. In addition, in the mode of classified pulping of the recycled paper, the high-quality recycled paper output rate can be greatly improved, added economic benefits can be brought to enterprises by combining with natural texture of the loofah sponge, and meanwhile the method has the special significance on environmental protection.

The invention relates to a method for fast reproducing pinellia ternate. The method comprises the following steps that a pinellia ternate seedball is taken, inducing growing culturing is carried out on an adventitious bud, a tissue block with adventitious bud clusters is obtained, and root induction culturing is carried out to obtain a pinellia ternate seedling. The method is high in regeneration efficiency, the bud ratio is about 100%, the pollution rate is lowered, repeated pollution is avoided in the culturing process, and inductivity can be improved and the regeneration period can be shortened by adjusting culture medium conditions.

The invention provides a method for directly obtaining plant through eggplant anther cultivation as well as a medium thereof, which belong to the technical field of plant tissue culture technique. The method comprises the following steps: 1) selecting fresh buds; 2) disinfecting the surface of bud and acquiring the aseptic anther; 3) inoculating the aseptic anther in the late uninucleate stage in an anther culture solid medium, culturing the aseptic anther for 2-8 days at dark environment at the temperature of 33-37 DEG C, and performing illumination culture for 30-40 days at the temperature of 25-28 DEG C; and 4) transferring the cotyledonous embryoid to a healthy seedling solid medium for continuously culturing the materials, and growing to obtain the plant. The method has the following advantages that the method is simple and easy to carry out, the in-vitro culture of the anther can directly induce the embryoid to obtain the haploid plant, a condition that the callus formed by anther culture induction is transferred to a differentiation medium, and then the plant regeneration is induced can be avoided; and the chromosome ploidy mixing phenomenon due to participation of diploid somatic cells such as the anther wall in callus can be avoided.

The invention discloses a one-step plantlet formation medium and method for the isolated culture of lamiophlomis rotata. By adopting the one-step plantlet formation medium, a lamiophlomis rotata test-tube plantlet leaf forms a plantlet in one step through isolated culture. The one-step plantlet formation culture medium is characterized in that a MS (Murashige and Skoog) medium is used as a basic medium, and 6-benzylaminopurine (6-BA), naphthylacetic acid (NAA) and 2,4-dichlorphenoxyacetic acid (2,4-D) which are different in concentrations and combinations are added to the base medium. According to the one-step plantlet formation method, the test-tube plantlet leaf serving as an explant is inoculated, then is induced by a callus and is directly differentiated into an adventitious bud and an adventitious root, so that the lamiophlomis rotata regenerated plantlet is obtained by one step. By adopting the one-step plantlet formation method, the callus obtained by induction in an initial medium does not need to be transferred into a differential medium for regenerating the plantlet. Compared with an isolated culture multi-step regeneration plantlet formation method, the one-step plantlet formation method has the advantages that the steps are simplified, the culture period is short, the repeatability is good, and the planting percent is high.

The invention discloses an environmental-friendly desulfurization process of smoke. The desulfurization process comprises the steps of (1) dust elimination and cleaning of smoke: making smoke pass through a dedusting channel to eliminate a part of dust, and adding a scrubbing solution into the channel, wherein the scrubbing solution is prepared from, by weight, 40-50 parts of hydrogen peroxide, 5-10 parts of sodium hydroxide particles, 1-5 parts of dodecyl dimethyl betaine, and 1-3 parts of sodium carboxymethylcellulose; (2) primary removal of hydrogen sulfide: adopting iron oxide desulfurizer under a normal temperature in the primary removal of hydrogen sulfide; (3) first-level fine desulfurization: adopting active carbon desulfurizer under a normal temperature in the first-level fine desulfurization; (4) second-level fine desulfurization: adopting molecular sieve desulfurizer in the second-level fine desulfurization. The multiple types of organic sulfur adsorbent adopted in the environmental-friendly desulfurization process of smoke are all reproducible and capable of being recycled, and the environmental-friendly desulfurization process of smoke has the advantages of getting rid of interference conducted on adsorption and desorption of absorbent by inorganic sulfur impurities, improving efficiency of desulfurization, saving energy to protect environment, decreasing consumption, and increasing the purity and cleanliness of smoke after desulfurization.

The invention relates to a one-step seedling emergence and quick propagation method for the tissue culture of sedum aizoon, and belongs to the technical field of plant tissue culture. Through the adoption of the method disclosed by the invention, the problems that the coefficient of a conventional propagation method for the sedum aizoon is low, the growth is slow, and the large-scale production is difficult to form are solved. The method comprises the following steps: (1) performing sterilization and disinfection; (2) performing one-step seedling emergence culture; (3) domesticating and transplanting test-tube plantlets, wherein rooting seedlings are taken out and are put in a seedling exercising room with enough illumination, after 3-5 days, bottle mouths are opened, seedlings are continuously exercised for 2-3 days, then the test-tube plantlets are taken out, root culture mediums are cleaned and are transplanted in substrates consisting of peat soil and vermiculite, the ratio of the weight parts of the peat soil to the weight parts of the vermiculite is 1: 1, and the condition of spraying small seedlings 2-3 times a day for first 7 days is ensured. The method disclosed by the invention is used for the tissue culture of the sedum aizoon.

The invention discloses a melon regeneration in vitro method. The melon regeneration in vitro method provided by the invention comprises the following steps: (1) taking a cotyledon of a melon as an explant, inoculating the explant into a shoot induction medium for culturing to obtain an adventitious bud tussock; (2) placing the adventitious bud tussock obtained in the step (1) to a shoot elongation medium A for culturing to obtain an adventitious bud; (3) placing the adventitious bud obtained in the step (2) into a rooting medium for culturing to obtain a melon regeneration seedling. The melon regeneration in vitro method provided by the invention has the advantages of high adventitious bud inductivity, low vitrification degree, short regeneration period and the like, and by applying the method in the melon genetic transformation, a relatively high transformation rate can be obtained.