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In-vitro rapid propagation method for lycoris radiata

A technology of lycoris in vitro and in vitro is applied to the field of rapid in vitro propagation of lycoris, which can solve the problems of increased workload, repeated pollution, and bulb carrying bacteria, and achieves the effects of shortening the regeneration period, increasing the regeneration efficiency, and improving the induction rate.

Inactive Publication Date: 2016-03-09
韦丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is relatively convenient to use bulbs as explants for regeneration, but due to the serious bacterial contamination of bulbs, it often leads to incomplete disinfection, repeated pollution during the cultivation process is very serious, increasing the workload, and the regeneration efficiency of scales as explants is low. Not very ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for rapid propagation of lycoris in vitro, comprising the steps of:

[0022] 1) Take Lycoris bulbs planted in the open field, disinfect them, remove the epiphytic seed bulbs near the cue bulb and plant them for 1 month;

[0023] 2) After disinfecting the bulbs obtained in step 1), remove the outer cortex, peel off the seed buds, place them flat on MS solid medium to induce growth of adventitious buds, and obtain tissue blocks with adventitious bud clusters;

[0024] 3) making the tissue block obtained in step 2) into a longitudinally divided tissue block, and carrying out bud elongation culture on the bud elongation medium;

[0025] 4) when the adventitious buds obtained in step 3) elongate to produce side buds, cut them;

[0026] 5) Putting the single stem cut in step 4) into the rooting medium to induce rooting culture to obtain Lycoris seedlings.

[0027] The MS solid medium is MS+BA1.8mg / L+NAA0.8mg / L, pH5.8, sucrose 28g / L, agar 4g / L.

[0028] The shoot e...

Embodiment 2

[0034] A method for rapid propagation of lycoris in vitro, comprising the steps of:

[0035] 1) Take Lycoris bulbs planted in the open field, disinfect them, remove the epiphytic seed bulbs near the cue bulb and plant them for 1.5 months;

[0036] 2) After disinfecting the bulbs obtained in step 1), remove the outer cortex, peel off the seed buds, place them flat on MS solid medium to induce growth of adventitious buds, and obtain tissue blocks with adventitious bud clusters;

[0037] 3) making the tissue block obtained in step 2) into a longitudinally divided tissue block, and carrying out bud elongation culture on the bud elongation medium;

[0038] 4) when the adventitious buds obtained in step 3) elongate to produce side buds, cut them;

[0039] 5) Putting the single stem cut in step 4) into the rooting medium to induce rooting culture to obtain Lycoris seedlings.

[0040] The MS solid medium is MS+BA2.2mg / L+NAA1.2mg / L, pH5.8, sucrose 32g / L, agar 6g / L.

[0041] The shoo...

Embodiment 3

[0047] A method for rapid propagation of lycoris in vitro, comprising the steps of:

[0048] 1) Take Lycoris bulbs planted in the open field, disinfect them, remove the epiphytic seed bulbs near the cue bulb and plant them for 2 months;

[0049] 2) After disinfecting the bulbs obtained in step 1), remove the outer cortex, peel off the seed buds, place them flat on MS solid medium to induce growth of adventitious buds, and obtain tissue blocks with adventitious bud clusters;

[0050] 3) making the tissue block obtained in step 2) into a longitudinally divided tissue block, and carrying out bud elongation culture on the bud elongation medium;

[0051] 4) when the adventitious buds obtained in step 3) elongate to produce side buds, cut them;

[0052] 5) Putting the single stem cut in step 4) into the rooting medium to induce rooting culture to obtain Lycoris seedlings.

[0053] The MS solid medium is MS+BA 2.0 mg / L+NAA 1.0 mg / L, pH 5.8, sucrose 30 g / L, agar 5 g / L.

[0054] The...

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Abstract

The invention relates to an in-vitro rapid propagation method for lycoris radiata. The method comprises the steps of: taking a bulb of lycoris radiata which is planted in an open field, disinfecting the bulb, and taking down epiphytic cormels near a mother bulb and planting the cormels for 1-2 months; after the bulb is disinfected, removing the outer skin, peeling seed buds, and performing inductive growth culture of adventitious buds to obtain a tissue block with adventitious buds; making the obtained tissue block into a longitudinal tissue block, and performing bud elongation culture on the longitudinal tissue block in a bud elongation culture medium; and when the obtained adventitious buds elongate and generate side buds, cutting the adventitious buds and putting the adventitious buds in a rooting culture medium for inductive rooting culture to obtain lycoris radiata seedlings. The young and tender buds of lycoris radiata are used as explants which directly generate a lot of adventitious buds so as to further obtain regenerated seedlings, so that the regenerating efficiency is increased, the sprouting ratio is not less than 90%, the contamination rate is low, the situation of repeated contamination in a culture process is avoided, and the induction rate can be increased and the regenerating period can be shortened by adjusting the conditions of the culture medium.

Description

technical field [0001] The invention relates to a method for breeding and expanding seeds of a biological medicinal material Lycoris, and the technology is directly applied to the breeding and expanding of biological medicinal materials, in particular to a method for in vitro rapid propagation of Lycoris. Background technique [0002] Amaryllis is a common garden ornamental plant in East Asia. Its leaves are admired in winter and its flowers are admired in autumn. It is an excellent perennial herbaceous flower, often used in gardens as shade greening or understory ground cover flowers, flower border cluster planting or natural planting between mountains and rocks. Because of its leaves when it blooms, it should be matched with other brighter herbaceous plants. It can be used as flower bed or flower path material, and it is also a beautiful cut flower. [0003] Lycoris scales are the most commonly used tissue culture materials for rapid propagation. Studies have shown that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 韦丽
Owner 韦丽
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